本文選題：腦性癱瘓 + 腫瘤壞死因子-α　； 參考：《新鄉(xiāng)醫(yī)學(xué)院》2017年碩士論文

【摘要】：背景腦性癱瘓(cerebral palsy,CP)是一組持續(xù)存在的中樞性運(yùn)動(dòng)和姿勢(shì)發(fā)育障礙,活動(dòng)受限癥侯群,這種癥候群是由于發(fā)育中的胎兒或嬰幼兒腦部非進(jìn)行性腦損傷所致,腦性癱瘓的運(yùn)動(dòng)障礙常伴有感覺(jué),知覺(jué),認(rèn)知,交流和行為障礙,以及癲癇和繼發(fā)性肌肉骨骼問(wèn)題。隨著年齡的增長(zhǎng),腦癱患兒機(jī)體運(yùn)動(dòng)、舉止和智力等相關(guān)障礙異常加劇,并將伴隨患兒一生,因此患兒生活品質(zhì)遠(yuǎn)低于正常兒童,對(duì)患兒家庭來(lái)說(shuō)也將是一個(gè)沉重的負(fù)擔(dān)。自腦性癱瘓這一概念形成之日起,醫(yī)務(wù)工作者以及相關(guān)科學(xué)家們一直棄而不舍對(duì)其病因?qū)W進(jìn)行深入地研究,但是,由于該疾病的病因及病理機(jī)制復(fù)雜多樣,并且臨床特征多種多樣,具體的病因和病理學(xué)機(jī)制目前尚不清楚。目前認(rèn)為除了大腦原發(fā)性損傷外,腦組織損傷的過(guò)程可持續(xù)數(shù)月至數(shù)年,即腦損傷的三級(jí)機(jī)制。三級(jí)腦損傷是持續(xù)慢性炎癥反應(yīng)和表觀遺傳基因的改變。為深入探討腦性癱瘓患兒的腦損傷的發(fā)病機(jī)制,該課題通過(guò)測(cè)定兩組不同年齡組腦癱患兒及相應(yīng)正常兒童血清中腫瘤壞死因子-α(TNF-α)、白介素-6(IL-6)、白介素-10(IL-10)的含量,探討腦性癱瘓患兒血清內(nèi)炎癥細(xì)胞因子TNF-α、IL-6、IL-10含量的變化。目的通過(guò)比較不同年齡組腦癱患兒及正常健康兒童血清中的TNF-α、IL-6、IL-10的表達(dá)水平,探討IL-6、IL-10和TNF-α參與腦性癱瘓患兒腦損傷的相關(guān)性研究。方法采用前瞻性研究的方法,選取新鄉(xiāng)醫(yī)學(xué)院第一附屬醫(yī)院小兒康復(fù)科腦癱患兒50例,其中嬰兒組患兒[6~12(8.4±1.5)月]25例、幼兒組患兒[13~36(22.8±6.1)月]25例為觀察組,正常兒童組30例為對(duì)照組,其中嬰兒組[6~12(8.2±1.3)月]15例、幼兒組患兒[13~36(23.2±6.2)月]15例。采用ELISA法檢測(cè)血清TNF-α、IL-6、IL-10的水平。結(jié)果1.觀察組全組腦癱患兒血清TNF-α、IL-6、IL-10水平較對(duì)照組全組正常兒童血清TNF-α、IL-6、IL-10水平明顯增高(P0.0001)。2.觀察組中嬰兒組、幼兒組TNF-α、IL-10水平較相應(yīng)年齡對(duì)照組明顯升高(P0.0001);觀察組中嬰兒組IL-6水平較對(duì)照組明顯升高(P=0.0011);觀察組中幼兒組IL-6水平較對(duì)照組明顯升高(P=0.0002)。3.觀察組中嬰兒組與幼兒組TNF-α水平比較無(wú)明顯差異(P=0.624);觀察組中嬰兒組與幼兒組IL-6水平比較無(wú)明顯差異(P=0.571);觀察組中嬰兒組與幼兒組IL-10水平比較無(wú)明顯差異(P=0.572)。結(jié)論腦性癱瘓患兒血清中炎癥因子TNF-α、IL-6、IL-10的水平明顯高于對(duì)照組,提示這三種細(xì)胞因子可能參與了腦癱患兒腦損傷的發(fā)生;腦癱組中嬰兒組與幼兒組血清中TNF-α、IL-6、IL-10水平無(wú)明顯變化,提示這三種細(xì)胞因子在腦性癱瘓患兒體內(nèi)持續(xù)表達(dá),說(shuō)明炎癥反應(yīng)在腦性癱瘓的發(fā)病中可能持續(xù)存在。
[Abstract]:Background Cerebral palsy is a group of persistent central motor and postural dysplasia, a group of dyskinesia, which is caused by non-progressive brain damage in developing fetuses or infants. Motor disorders of cerebral palsy are often associated with sensory, perceptual, cognitive, communication, and behavioral disorders, as well as epilepsy and secondary musculoskeletal problems. With the increase of age, the children with cerebral palsy and other related disorders such as motor, behavior and intelligence will be aggravated, and will accompany the children for life, so the quality of life of the children is far lower than the normal children, and it will be a heavy burden to the children's families. Since the formation of the concept of cerebral palsy, medical workers and related scientists have been reluctant to study the etiology of cerebral palsy. However, the etiology and pathological mechanisms of the disease are complicated and varied. And the clinical characteristics are varied, the specific etiology and pathological mechanism is not clear. It is believed that in addition to primary brain injury, the process of brain tissue injury can last several months to several years, that is, the tertiary mechanism of brain injury. Third-level brain injury is a persistent chronic inflammatory response and epigenetic changes. In order to investigate the pathogenesis of cerebral injury in children with cerebral palsy, the contents of tumor necrosis factor- 偽 (TNF- 偽), interleukin-6 (IL-6) and interleukin-10 (IL-10) in the serum of children with cerebral palsy of different age groups and corresponding normal children were measured. To investigate the changes of serum inflammatory cytokines TNF- 偽 and IL-6 + IL-10 in children with cerebral palsy. Objective to study the relationship between the expression of TNF- 偽 and IL-6 and IL-10 in cerebral palsy children with cerebral palsy and the correlation between TNF- 偽 and TNF- 偽 in cerebral injury in children with cerebral palsy by comparing the levels of TNF- 偽 and IL-10 in the serum of children with cerebral palsy. Methods A prospective study was conducted in 50 children with cerebral palsy in the Department of Pediatric Rehabilitation in the first affiliated Hospital of Xinxiang Medical College, including 25 infants in the infant group [6128.4 鹵1.5 months], 25 children in the infant group [1336362.8 鹵6.1 months] and 30 normal children as the control group. There were 15 cases in the infant group [6 ~ 12 鹵8.2 鹵1.3 months] and 15 cases in the infant group [13 ~ 3623.2 鹵6.2) months]. Serum TNF- 偽 IL-6 and IL-10 levels were detected by ELISA method. Result 1. The serum levels of TNF- 偽 and IL-6 + IL-10 in children with cerebral palsy in the observation group were significantly higher than those in the control group (P = 0.0001 / 0. 2). The levels of serum TNF- 偽 and IL-6 / IL-10 in children with cerebral palsy were significantly higher than those in the control group (P < 0.05). The levels of TNF- 偽 and IL-10 in infants in the observation group were significantly higher than those in the control group (P 0.0001), the level of IL-6 in the observation group was significantly higher than that in the control group (0.0011), and the level of IL-6 in the observation group was significantly higher than that in the control group. There was no significant difference in the levels of TNF- 偽 between the infant group and the infant group, there was no significant difference in the level of IL-6 between the infant group and the infant group in the observation group, and there was no significant difference in the level of IL-10 between the infant group and the infant group. There was no significant difference in the level of IL-10 between the observation group and the infant group. Conclusion the level of TNF- 偽 IL-6 IL-10 in children with cerebral palsy is significantly higher than that in the control group, suggesting that these three cytokines may be involved in the development of cerebral injury in children with cerebral palsy, while the levels of TNF- 偽 IL-6IL-10 in serum of infants and infants with cerebral palsy have no significant change. These three cytokines were continuously expressed in children with cerebral palsy, indicating that inflammatory reaction may persist in the pathogenesis of cerebral palsy.
【學(xué)位授予單位】：新鄉(xiāng)醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R742.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 桑鑫泉;程遠(yuǎn);李多多;柳曉燕;朱鳳蓮;;EGFR抑制劑C225在宮內(nèi)感染仔鼠腦組織炎癥中的實(shí)驗(yàn)研究[J];中國(guó)婦幼保健;2015年27期

2 鄧濤;何善輝;錢(qián)涪屏;劉啟潔;唐建軍;;血清IL-10水平與腦損傷及預(yù)后的關(guān)系以及NDT對(duì)其的影響[J];海南醫(yī)學(xué)院學(xué)報(bào);2015年10期

3 李曉捷;唐久來(lái);馬丙祥;秦炯;鄒麗萍;王家勤;;腦性癱瘓的定義、診斷標(biāo)準(zhǔn)及臨床分型[J];中華實(shí)用兒科臨床雜志;2014年19期

4 張鴻;文芳;陶維元;吳擎;;在腦癱發(fā)生過(guò)程中血清IL-10水平的變化及臨床意義[J];卒中與神經(jīng)疾病;2010年03期

相關(guān)博士學(xué)位論文 前2條

1 李宏;腦性癱瘓病因及其修復(fù)機(jī)制的系列研究[D];南方醫(yī)科大學(xué);2013年

2 程秀永;小兒腦性癱瘓的臨床與分子遺傳學(xué)研究[D];復(fù)旦大學(xué);2010年

，

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