本文選題：急性心肌梗死 + 急性腦梗塞�。� 參考：《中國醫(yī)科大學學報》2017年08期

【摘要】：目的確定一種可以作為急性心肌梗死(AMI)或急性腦梗死(ACI)疾病診斷標志物的新的mi RNA組合。方法根據(jù)文獻與公開的互聯(lián)網(wǎng)數(shù)據(jù)庫,選擇高頻出現(xiàn)的mi RNA做為候選基因,收集AMI患者外周血樣本13例、ACI患者外周血樣本11例和健康志愿者樣本20例。利用試劑盒提取這些樣本中的游離RNA,進行反轉(zhuǎn)錄及實時定量PCR測定,檢測所有樣本中候選mi RNA的表達情況。對2個疾病組和健康對照組中候選mi RNA的表達差異進行統(tǒng)計學分析。結(jié)果經(jīng)過篩查,AMI組中出現(xiàn)頻率最高的mi RNA有mi RNA1、mi RNA499a和mi RNA133a,ACI組中出現(xiàn)頻率最高的mi RNA有l(wèi)et7i、mi RNA16和mi RNA223。2個疾病組中6種mi RNA的表達量均顯著高于健康對照組(P0.01)。AMI組mi RNA1和mi RNA499a的表達量分別為ACI組的4.82倍和3.50倍(P0.01),而mi RNA133a的表達量無統(tǒng)計學差異(P0.05)。ACI組與AMI組比較,mi RNA16和mi RNA233的表達量無統(tǒng)計學差異(P0.05),ACI組let7i的表達量為AMI組的2.61倍(P0.05)。結(jié)論人外周血游離RNA中6種mi RNA均異常升高可以作為AMI或ACI的初步診斷標志物,在此基礎(chǔ)上,mi RNA1和mi RNA499a的表達量達到11~20倍以上時,對AMI的發(fā)病具有輔助診斷及預警作用;let7i的表達量達到6倍以上時,對ACI的發(fā)病具有輔助診斷及預警作用。
[Abstract]:Objective to identify a new mi RNA combination for diagnosis of acute myocardial infarction (AMI) or acute cerebral infarction (ACI). Methods according to the literature and the open Internet database, the high frequency RNA was selected as candidate gene. The peripheral blood samples of 13 patients with AMI and 20 healthy volunteers were collected. Free RNAs from these samples were extracted by kit, reverse transcription and real-time quantitative PCR were performed to detect the expression of candidate mi RNA in all samples. The difference of the expression of candidate mi RNA between the two disease groups and the healthy control group was analyzed statistically. Results after screening, the highest frequency of mi RNA was found in the group of mi RNA1, mi RNA499a and mi RNA133aACI, and the expression of the six kinds of RNA in the group with the highest frequency of miRNA1 RNA16 and RNA223.2 was significantly higher than that in the healthy control group (P 0.01). The expression of RNA1 in the group of AMI was significantly higher than that in the group of healthy controls (P 0.01), and the expression of RNA1 in the group of RNA223.2 was significantly higher than that in the group of healthy controls (P 0.01). The expression of mi RNA499a was 4.82 times and 3.50 times as much as that of ACI group (P 0.01), but there was no significant difference in the expression of mi RNA133a between the two groups. There was no significant difference in the expression of RNA16 and mi RNA233 between P0.05ACI group and AMI group. The let7i expression in P0.05ACI group was 2.61 times higher than that in AMI group. Conclusion the abnormal elevation of six kinds of mi RNA in human peripheral blood free RNA can be used as a preliminary diagnostic marker for AMI or ACI. On this basis, the expression of mi RNA1 and mi RNA499a is more than 1120 times. When the expression of let7i is more than 6 times of the expression level of AMI, it has auxiliary diagnosis and early warning effect on the pathogenesis of ACI.
【作者單位】： 大連醫(yī)科大學附屬第二醫(yī)院創(chuàng)傷急癥中心;大連醫(yī)科大學附屬第一醫(yī)院神經(jīng)內(nèi)科;
【基金】：國家自然科學基金(81672968)
【分類號】：R542.22;R743.3
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本文編號：1857633