本文選題：白藜蘆醇 + SOD-GA小鼠　； 參考：《中藥藥理與臨床》2015年06期

【摘要】：目的:研究白藜蘆醇對(duì)SOD1-G93A小鼠腰髓和運(yùn)動(dòng)皮層LC3B的影響。方法:36只SOD1-G93A小鼠隨機(jī)分為3組,對(duì)照組、溶劑組、白藜蘆醇組,每組12只,至癥狀早期(給藥20~40天)或者終末期(給藥60~80天)取材,利用激光共聚焦、免疫組織化學(xué)、Western-blot技術(shù)檢測(cè)小鼠腰髓和運(yùn)動(dòng)皮層中LC3B的表達(dá)及LC3BⅠ/Ⅱ比值變化。結(jié)果:激光共聚焦技術(shù)及免疫組織化學(xué)技術(shù)檢測(cè)發(fā)現(xiàn)癥狀早期白藜蘆醇組(30mg/kg)和溶劑組小鼠腰髓運(yùn)動(dòng)神經(jīng)元及運(yùn)動(dòng)皮層神經(jīng)元與對(duì)照組相比LC3B表達(dá)增多;Western blot技術(shù)檢測(cè)發(fā)現(xiàn)癥狀早期白藜蘆醇組(30mg/kg/d)小鼠腰髓和運(yùn)動(dòng)皮層神經(jīng)元中LC3BⅠ/Ⅱ比值分別為(1.20±0.29,1.27±0.30),與對(duì)照組相比(1.29±0.34,1.42±0.27)降低,但無統(tǒng)計(jì)學(xué)差異(P0.05)。終末期三組間小鼠腰髓和運(yùn)動(dòng)皮層LC3B表達(dá)及LC3BⅠ/Ⅱ比值無明顯差異。結(jié)論:白藜蘆醇對(duì)SOD1-G93A小鼠腰髓和運(yùn)動(dòng)皮層LC3B的影響微弱。
[Abstract]:Objective: to study the effect of resveratrol on LC3B in lumbar spinal cord and motor cortex of SOD1-G93A mice. Methods Thirty six SOD1-G93A mice were randomly divided into three groups: control group, solvent group, resveratrol group, 12 rats in each group, and were collected from the early stage of symptoms (2040 days) or the end stage (6080 days). The expression of LC3B and the ratio of LC3B 鈪，

本文編號(hào)：1855500