發(fā)布時間：2018-05-01 02:22

  本文選題：肌衛(wèi)星細胞 + 可塑性��； 參考：《生物化學與生物物理進展》2016年06期

【摘要】：肌衛(wèi)星細胞在骨骼肌生長發(fā)育和出生后骨骼肌損傷修復中起著重要的作用,但是有關(guān)肌萎縮中肌衛(wèi)星細胞的可塑性變化、作用及其機制尚不清楚.本研究采用小鼠尾懸吊模擬失重效應誘導失重肌萎縮,動態(tài)分析了失重肌萎縮發(fā)生過程中不同類型肌纖維的肌衛(wèi)星細胞數(shù)量和增殖、分化潛能可塑性的改變,發(fā)現(xiàn)在失重肌萎縮過程中,處于安靜狀態(tài)的肌衛(wèi)星細胞顯著增多、激活增殖的肌衛(wèi)星細胞顯著減少,而具有成肌分化潛能的肌衛(wèi)星細胞有持續(xù)減少趨勢.此外,在失重肌萎縮比目魚肌單根肌纖維移出的體外培養(yǎng)中,證明了失重肌萎縮肌纖維肌衛(wèi)星細胞可塑性降低的特征性變化.進一步,通過對比分析Smad3基因敲除及其同窩野生型小鼠,在失重肌萎縮中肌衛(wèi)星細胞可塑性的差異性變化,揭示了Smad3在調(diào)控失重肌萎縮肌衛(wèi)星細胞可塑性變化中的關(guān)鍵作用.
[Abstract]:Muscle satellite cells play an important role in the growth and development of skeletal muscle and the repair of skeletal muscle injury after birth. However, the plasticity of muscle satellite cells in muscle atrophy and its mechanism are not clear. In this study, weightlessness muscle atrophy was induced by tail suspension simulated weightlessness in mice. The number and proliferation of muscle satellite cells of different types of muscle fibers and the plasticity of differentiation potential were dynamically analyzed during the occurrence of weightlessness muscle atrophy. It was found that during the course of weightlessness muscle atrophy, the number of mysatellite cells in quiet state increased significantly, the number of activated and proliferated mysatellite cells decreased significantly, while the number of mysatellite cells with myogenic differentiation potential continued to decrease. In addition, in vitro culture of muscle fibers removed from soleus muscle of weightlessness muscle atrophy, the characteristic change of plasticity reduction of muscle satellite cells of muscle fiber of weightlessness muscle atrophy was proved. Furthermore, by comparing and analyzing the difference of plasticity of muscle satellite cells in weightlessness muscle atrophy in Smad3 knockout and its same litter wild-type mice, the key role of Smad3 in regulating the plasticity of weightlessness muscle atrophy muscle satellite cells was revealed.
【作者單位】： 陜西師范大學生命科學學院;中國航天員科研訓練中心航天醫(yī)學基礎(chǔ)與應用國家重點實驗室;中國航天員科研訓練中心人因工程重點實驗室;
【基金】：國家自然基金(31171144,81272177) 航天醫(yī)學基礎(chǔ)與應用國家重點實驗室基金(SMFA13A01) 人因工程重點實驗室基金(SYFD140051801)資助項目~~
【分類號】：R746.4

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