本文選題：肌衛(wèi)星細(xì)胞 + 可塑性　； 參考：《生物化學(xué)與生物物理進(jìn)展》2016年06期

【摘要】：肌衛(wèi)星細(xì)胞在骨骼肌生長(zhǎng)發(fā)育和出生后骨骼肌損傷修復(fù)中起著重要的作用,但是有關(guān)肌萎縮中肌衛(wèi)星細(xì)胞的可塑性變化、作用及其機(jī)制尚不清楚.本研究采用小鼠尾懸吊模擬失重效應(yīng)誘導(dǎo)失重肌萎縮,動(dòng)態(tài)分析了失重肌萎縮發(fā)生過(guò)程中不同類(lèi)型肌纖維的肌衛(wèi)星細(xì)胞數(shù)量和增殖、分化潛能可塑性的改變,發(fā)現(xiàn)在失重肌萎縮過(guò)程中,處于安靜狀態(tài)的肌衛(wèi)星細(xì)胞顯著增多、激活增殖的肌衛(wèi)星細(xì)胞顯著減少,而具有成肌分化潛能的肌衛(wèi)星細(xì)胞有持續(xù)減少趨勢(shì).此外,在失重肌萎縮比目魚(yú)肌單根肌纖維移出的體外培養(yǎng)中,證明了失重肌萎縮肌纖維肌衛(wèi)星細(xì)胞可塑性降低的特征性變化.進(jìn)一步,通過(guò)對(duì)比分析Smad3基因敲除及其同窩野生型小鼠,在失重肌萎縮中肌衛(wèi)星細(xì)胞可塑性的差異性變化,揭示了Smad3在調(diào)控失重肌萎縮肌衛(wèi)星細(xì)胞可塑性變化中的關(guān)鍵作用.
[Abstract]:Muscle satellite cells play an important role in the growth and development of skeletal muscle and the repair of skeletal muscle injury after birth. However, the plasticity of muscle satellite cells in muscle atrophy and its mechanism are not clear. In this study, weightlessness muscle atrophy was induced by tail suspension simulated weightlessness in mice. The number and proliferation of muscle satellite cells of different types of muscle fibers and the plasticity of differentiation potential were dynamically analyzed during the occurrence of weightlessness muscle atrophy. It was found that during the course of weightlessness muscle atrophy, the number of mysatellite cells in quiet state increased significantly, the number of activated and proliferated mysatellite cells decreased significantly, while the number of mysatellite cells with myogenic differentiation potential continued to decrease. In addition, in vitro culture of muscle fibers removed from soleus muscle of weightlessness muscle atrophy, the characteristic change of plasticity reduction of muscle satellite cells of muscle fiber of weightlessness muscle atrophy was proved. Furthermore, by comparing and analyzing the difference of plasticity of muscle satellite cells in weightlessness muscle atrophy in Smad3 knockout and its same litter wild-type mice, the key role of Smad3 in regulating the plasticity of weightlessness muscle atrophy muscle satellite cells was revealed.
【作者單位】： 陜西師范大學(xué)生命科學(xué)學(xué)院;中國(guó)航天員科研訓(xùn)練中心航天醫(yī)學(xué)基礎(chǔ)與應(yīng)用國(guó)家重點(diǎn)實(shí)驗(yàn)室;中國(guó)航天員科研訓(xùn)練中心人因工程重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然基金(31171144,81272177) 航天醫(yī)學(xué)基礎(chǔ)與應(yīng)用國(guó)家重點(diǎn)實(shí)驗(yàn)室基金(SMFA13A01) 人因工程重點(diǎn)實(shí)驗(yàn)室基金(SYFD140051801)資助項(xiàng)目~~
【分類(lèi)號(hào)】：R746.4

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