本文選題：博爾納病病毒 + 抗體�。� 參考：《遵義醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的：了解博爾納病病毒（BDV）在貴州省部分地區(qū)人群中的的感染情況，探討博爾納病病毒與病毒性腦炎（VE）、多發(fā)性硬化（MS）、吉蘭-巴雷綜合征（GBS）以及帕金森病（PD）等神經(jīng)系統(tǒng)疾病的相關(guān)性。 方法：在2012年10月-2013年6月期間，收集遵義醫(yī)學(xué)院附屬醫(yī)院、貴州省人民醫(yī)院、貴陽醫(yī)學(xué)院附屬醫(yī)院、貴陽市第二人民醫(yī)院、貴陽中醫(yī)學(xué)院第二附屬醫(yī)院等大型綜合醫(yī)院神經(jīng)內(nèi)科住院病人中，診斷為不明原因的病毒性腦炎（65例）、多發(fā)性硬化（7例）、吉蘭-巴雷綜合征（9例）、帕金森�。�7例）外周血液樣本，共計88例；另外收集遵義醫(yī)學(xué)院附屬醫(yī)院體檢科健康體檢患者外周血液樣本93例。并運用“三明治夾心”ELISA法，檢測兩組樣本的BDV-CIC，并對陽性樣本進行BDV抗體檢測。 結(jié)果：88例神經(jīng)疾病組中有11例血漿中BDV-CIC為陽性，陽性率為12.6%（11/88）；93例對照組中BDV-CIC陽性7例，陽性率為7.5（7/93）；神經(jīng)系統(tǒng)疾病組中病毒性腦炎BDV-CIC檢出率為9.2%（6/65），與對照組差異無明顯統(tǒng)計學(xué)意義（P0.05）；多發(fā)性硬化BDV-CIC檢出率為28.6%（2/7），與對照組差異無明顯統(tǒng)計學(xué)意義（P0.05）；吉蘭-巴雷綜合征BDV-CIC檢出率為33.3%（3/9），與對照組差異有統(tǒng)計學(xué)意義（P0.05）；帕金森病BDV-CIC檢測率為0%（0/7），，與對照組差異無明顯統(tǒng)計學(xué)意義（P0.05）；神經(jīng)系統(tǒng)疾病組BDV-CIC總體檢出率高于健康對照組，但是差異無明顯統(tǒng)計學(xué)意義（P0.05）；神經(jīng)系統(tǒng)疾病組11例BDV-CIC陽性樣本中BDV抗體陽性6例，雙陽性率為6/11（54.5%），健康對照組7例BDV-CIC陽性樣本中BDV抗體陽性5例，雙陽性率為71.4%（5/7）。 結(jié)論：貴州省部分地區(qū)人群中存在著BDV的感染；本次研究中病毒性腦炎、多發(fā)性硬化、吉蘭-巴雷綜合征患者中存在BDV的感染，BDV感染與吉蘭-巴雷綜合征的發(fā)生可能相關(guān)；與病毒性腦炎、多發(fā)性硬化、帕金森病的發(fā)生不一定相關(guān)。
[Abstract]:Objective: to investigate the infection of Borna disease virus (DVV) in some areas of Guizhou province. To investigate the correlation between Borna disease virus and viral encephalitis (VEC), multiple sclerosis (MS), Guillain-Barre syndrome (GBS) and Parkinson's disease (PD). Methods: from October 2012 to June 2013, affiliated hospitals of Zunyi Medical College, Guizhou Provincial people's Hospital, Guiyang Medical College affiliated Hospital, Guiyang second people's Hospital were collected. Among the inpatients in the Department of Neurology, the second affiliated Hospital of Guiyang College of traditional Chinese Medicine, and other large general hospitals, The peripheral blood samples of 65 cases of viral encephalitis diagnosed as unknown cause, 7 cases of multiple sclerosis, 9 cases of Guillain-Barre syndrome and 7 cases of Parkinson's disease) were collected. In addition, 93 samples of peripheral blood fluid were collected from healthy patients in Department of physical examination of affiliated Hospital of Zunyi Medical College. Using sandwich sandwich ELISA method, the BDV-CICs of two groups of samples were detected, and the positive samples were tested for BDV antibody. Results among 88 cases of neuropathy, 11 cases were positive for BDV-CIC in plasma, and the positive rate was 12. 6%. 7 cases were positive for BDV-CIC in the control group of 11% 88%. The positive rate of viral encephalitis in patients with nervous system diseases was 9.5% / 93%, the positive rate of viral encephalitis was 9.2 / 65%, which had no significant difference from the control group (P 0.05), the detection rate of multiple sclerosis BDV-CIC was 28.610 / 7% (P 0.05), and there was no significant difference between the two groups (P 0.05). The detection rate of BDV-CIC in syndrome was 33. 3 / 9. The difference was statistically significant compared with the control group (P 0. 05). The detection rate of BDV-CIC in Parkinson's disease was 0 / 7. There was no significant difference between the two groups (P 0. 05). The overall detection rate of BDV-CIC in patients with nervous system diseases was higher than that in healthy controls. But there was no significant difference in BDV antibody in 11 BDV-CIC positive samples, 6 cases were positive for BDV antibody, and the double positive rate was 6 / 1154.5%. In the healthy control group, there were 5 cases of BDV antibody positive and 71.4% / 7% positive rate of BDV antibody in 7 BDV-CIC positive samples. Conclusion: BDV infection may be associated with BDV infection in patients with viral encephalitis, multiple sclerosis and Guillain-Barre syndrome. It is not necessarily associated with viral encephalitis, multiple sclerosis, and Parkinson's disease.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R741

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