本文選題：MTHFR + C677T基因��； 參考：《吉林大學(xué)》2014年碩士論文

【摘要】：目的： 采用Meta分析的方法對(duì)Hcy代謝關(guān)鍵酶MTHFR、CBS基因與缺血性腦卒中的相關(guān)文獻(xiàn)進(jìn)行綜合分析，判定MTHFR、CBS基因突變與缺血性腦卒中發(fā)病的相關(guān)性及其關(guān)聯(lián)強(qiáng)度，為缺血性腦卒中高危人群的篩選及臨床預(yù)防決策提供理論依據(jù)。 方法： 計(jì)算機(jī)檢索Cochrane圖書館、EMbase、Pubmed三個(gè)英文數(shù)據(jù)庫，檢索中國(guó)生物醫(yī)學(xué)文獻(xiàn)數(shù)據(jù)庫（CBM)、相關(guān)期刊論文（CNKI）、萬方數(shù)據(jù)庫三個(gè)中文數(shù)據(jù)庫，并進(jìn)行手動(dòng)補(bǔ)充檢索其他文獻(xiàn)，檢索時(shí)間為建庫時(shí)間至2013年12月。建立嚴(yán)格統(tǒng)一的文獻(xiàn)排除與納入標(biāo)準(zhǔn)來篩選文獻(xiàn)，對(duì)文獻(xiàn)進(jìn)行質(zhì)量評(píng)價(jià)，提取數(shù)據(jù)，最后利用Revman5.2.0統(tǒng)計(jì)軟件進(jìn)行meta分析，并繪制森林圖和漏斗圖（用以評(píng)估發(fā)表偏倚）。 結(jié)果： 1、MTHFR C677T等位基因分析結(jié)果 初步篩查文章題目及摘要后得到文獻(xiàn)22篇，其中英文7篇、中文15篇，根據(jù)納入和排除標(biāo)準(zhǔn)最后得到英文3篇，中文11篇。 經(jīng)過meta分析可以得到I2=72%，說明存在實(shí)際的異質(zhì)性，故采用隨機(jī)性效應(yīng)模型，在MTHFR C677T等位基因中，基因型(TT+TC)/CC的OR值為1.37，95%CI為0.98到1.92，p＜0.0001。2、CBS T833C等位基因分析結(jié)果 根據(jù)納入和排除標(biāo)準(zhǔn)初步篩查文章題目及摘要后得到文獻(xiàn)19篇，其中英文文獻(xiàn)7篇、中文文獻(xiàn)12篇，最后得到英文文獻(xiàn)2篇，中文文獻(xiàn)7篇。 進(jìn)行Meta分析后可以得到I2=60%，說明存在實(shí)際的異質(zhì)性，故采用隨機(jī)性效應(yīng)模型，在CBS T833C等位基因中，基因型（CC+CT）/TT的OR值為1.83，，95%CI為1.20到2.80，P=0.01。 結(jié)論： 1、MTHFR C677T基因 在MTHFR C677T等位基因中，與CC型基因?qū)Ρ�，攜帶TC、TT基因型的人發(fā)生缺血性腦血管病風(fēng)險(xiǎn)明顯增高。 2、CBS T833C基因 在CBS T833C等位基因中，與TT型基因?qū)Ρ龋瑪y帶CT、CC基因型的人發(fā)生缺血性腦血管病風(fēng)險(xiǎn)明顯增高。
[Abstract]:Objective: Meta analysis was used to analyze the correlation between MTHFRN CBS gene and ischemic stroke, and to determine the correlation and intensity between MTHFR gene mutation and ischemic stroke. To provide theoretical basis for screening and clinical prevention decision of high risk population of ischemic stroke. Methods: Three English databases were searched by computer in Cochrane Library: Embase / Pubmed, Chinese Biomedical Literature Database (CBM), China Journal Full-text Database (CNKI), Wanfang Database (three Chinese databases), and other documents were retrieved manually. The retrieval time is from the time of building the database to December 2013. A strict and unified criterion of literature exclusion and inclusion was established to screen the literature, evaluate the quality of the literature, extract the data. Finally, the meta analysis was carried out by using Revman5.2.0 statistical software, and the forest map and funnel map were drawn (to evaluate the publication bias). Results: 1 result of allele analysis of MTHFR C677T After the title and summary of the preliminary screening articles, 22 papers were obtained, of which 7 were in English and 7 in English, 15 in Chinese, and 3 in English and 11 in Chinese according to the inclusion and exclusion criteria. After meta analysis, I _ 2 / 72 can be obtained, indicating the existence of actual heterogeneity. Therefore, the random effect model is used. In the MTHFR C677T allele, the OR value of genotype TTT TC)/CC is 1.37% 95 CI 0.98 to 1.92% P < 0.0001.2% CBS T833C allele analysis results. According to the criteria of inclusion and exclusion, 19 articles were selected, including 7 articles in Chinese and English, 12 in Chinese, 2 in English and 7 in Chinese. After Meta analysis, I _ 2 ~ (60) can be obtained, indicating the existence of actual heterogeneity. Therefore, the random effect model was used. In the CBS T833C allele, the OR value of CC CT)/TT was 1.83% 95 CI 1.20 to 2.80 P0. 01. Conclusion: 1. MTHFR C677T gene In the allele of MTHFR C677T, compared with CC genotype, the risk of ischemic cerebrovascular disease was significantly increased in people with TCT-TT genotype. CS-T833C gene In the allele of CBS T833C, compared with TT genotype, the risk of ischemic cerebrovascular disease was significantly increased in those with CTT833C genotype.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R743.3

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本文編號(hào)：1794547