本文選題：煙霧病 + 血管內(nèi)皮生長因子��； 參考：《泰山醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的 煙霧病是一種原因不明的慢性進(jìn)行性閉塞性腦血管疾病，主要表現(xiàn)為頸內(nèi)動脈遠(yuǎn)端和大腦前或中動脈近端狹窄或閉塞、伴腦底部異常血管網(wǎng)形成，其病因尚不明確。機(jī)體內(nèi)血管生成受多種細(xì)胞因子調(diào)控，根據(jù)其不同調(diào)控作用，可分為兩大類：促血管生成因子及血管生成抑制因子。促血管生成因子指的是具有刺激血管生長的蛋白質(zhì)分子或化合物。通過比較促血管生成因子中的血管內(nèi)皮生長因子(VEGF)和肝細(xì)胞生長因子(HGF)在煙霧病患者血清及腦脊液中同非煙霧病患者之間含量的差異，探索VEGF及HGF在煙霧病血管改變機(jī)制中的起到的作用。 方法 選取2013年1月至2013年12月在我院神經(jīng)外科住院的煙霧病患者24例為實驗組，所有患者均經(jīng)DSA檢查確診為煙霧病。同期選取因外傷導(dǎo)致蛛網(wǎng)膜下腔出血患者20例為對照組，該組患者均行顱腦MRI及經(jīng)顱多普勒超聲TCD檢查排除煙霧病可能。所有入組患者均行腰椎穿刺術(shù)留取2ml腦脊液標(biāo)本，均在清晨空腹時抽取肘靜脈血5ml于抗凝管內(nèi)，離心后留取血清標(biāo)本，采用ELISA方法檢測腦脊液及血清中VEGF及HGF的水平。 結(jié)果 1、在腦脊液組中，VEGF及HGF含量的表達(dá)在煙霧病組合對照組間有顯著性差異（p＜0.05），且在煙霧病組中的表達(dá)明顯高于對照組（p＜0.05）； 2、在血清組中，VEGF及HGF含量的表達(dá)在煙霧病組合對照組間有顯著性差異（p＜0.05），且在煙霧病組中的表達(dá)明顯高于對照組（p＜0.05）。 結(jié)論 煙霧病患者血清及腦脊液中VEGF及HGF含量均增高，，提示VEGF及HGF在促進(jìn)新生血管形成及保護(hù)營養(yǎng)神經(jīng)方面具有重要作用，參與了煙霧病的病理生理過程。
[Abstract]:Purpose Moyamoya disease is a chronic progressive occlusive cerebrovascular disease with unknown causes. The main manifestations of moyamoya disease are stenosis or occlusion of the distal end of the internal carotid artery and anterior or proximal cerebral artery, accompanied by abnormal vascular network at the base of the brain. The etiology of moyamoya disease is still unclear. Angiogenesis is regulated by many cytokines and can be divided into two categories: angiogenic factors and angiogenic inhibitory factors. An angiogenic factor is a protein or compound that stimulates the growth of blood vessels. By comparing the levels of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in serum and cerebrospinal fluid of moyamoya disease patients with moyamoya disease and those without moyamoya disease, the difference between VEGF and HGF in serum and cerebrospinal fluid of moyamoya disease patients was compared. To explore the role of VEGF and HGF in the pathogenesis of moyamoya disease. Method Twenty-four patients with moyamoya disease from January 2013 to December 2013 were selected as experimental group. All patients were diagnosed as moyamoya disease by DSA. Twenty patients with subarachnoid hemorrhage caused by trauma were selected as control group. All the patients were examined with craniocerebral MRI and transcranial Doppler TCD to eliminate the possibility of moyamoya disease. All the patients underwent lumbar puncture and 2ml cerebrospinal fluid (CSF) samples were collected. 5ml was collected from the elbow vein blood in the anticoagulant tube on an empty stomach in the morning. Serum samples were collected after centrifugation. The levels of VEGF and HGF in CSF and serum were detected by ELISA method. Result 1. The expression of HGF and HGF in CSF group was significantly higher than that in the control group (p < 0.05), and was significantly higher in the moyamoya disease group than in the control group (p < 0.05). 2. The expression of HGF and HGF in the serum group was significantly higher than that in the control group (p < 0.05), and the expression in the moyamoya disease group was significantly higher than that in the control group (p < 0.05). Conclusion The contents of VEGF and HGF in serum and cerebrospinal fluid of patients with moyamoya disease were increased, suggesting that VEGF and HGF play an important role in promoting neovascularization and protecting nutritional nerve, and participate in the pathophysiological process of moyamoya disease.
【學(xué)位授予單位】：泰山醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R743.3

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1 尤志s

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