本文選題：腦出血 + Caspase-7　； 參考：《右江民族醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:通過觀察在瑞舒伐他汀干預(yù)下大鼠實驗性腦出血(Intracerebral hemorrhage,ICH)血腫周圍腦組織中半胱氨酸蛋白酶3(cysteinylaspartate specific proteinases 3,Caspase-3)、半胱氨酸蛋白酶7(cysteinylaspartate specific proteinases 7,Caspase-7)和凋亡抑制蛋白1(cellular inhibitor of apoptosis proteins 1,c-IAP-1)的表達變化,探討瑞舒伐他汀在腦出血發(fā)生后可能出現(xiàn)的神經(jīng)保護作用及其機制,對臨床用藥起到一定指導(dǎo)作用。方法:(1)將60只SD大鼠按照隨機分組的方法分為假手術(shù)組(A組,20只),腦出血組(B組,20只),瑞舒伐他汀干預(yù)組(C組,20只)。每組分成6h、24h、72h、5d四個時間點,每個時間點使用5只大鼠進行造模并實驗。采用自體凝固血注入右側(cè)尾狀核法建立腦出血模型。(2)采用Garcia法對三組大鼠術(shù)后4個時間點分別進行神經(jīng)缺損評分。(3)免疫組化法對三組大鼠血腫周圍腦組織切片染色并在顯微鏡下觀察分析。(4)RT-qPCR技術(shù)測定三組大鼠腦出血術(shù)后4個時間點血腫周圍腦組織Caspase-3,-7及c-IAP-1表達狀況。結(jié)果:(1)Garcia評分評估發(fā)現(xiàn)6小時時間點腦出血組和瑞舒伐他汀干預(yù)組相比差異無統(tǒng)計學(xué)意義,其余三個時間點瑞舒伐他汀干預(yù)組(C組)神經(jīng)缺損情況較腦出血組(B組)減輕,P0.05,有統(tǒng)計學(xué)意義。兩組均在72h神經(jīng)缺損癥狀達到峰值,之后癥狀緩解。假手術(shù)組(A組)與其余兩組在術(shù)后4個時間點相比發(fā)現(xiàn)假手術(shù)組(A組)神經(jīng)缺損較輕,神經(jīng)功能遠高于其余兩組,差異均有統(tǒng)計學(xué)意義。(2)免疫組化法染色后顯微鏡下可觀察到假手術(shù)組(A組)4個時間點腦出血后c-IAP-1和Caspase-3,-7陽性細(xì)胞少量表達,腦出血組(B組)和瑞舒伐他汀干預(yù)組(C組)較假手術(shù)組(A組)每個時間點陽性細(xì)胞均出現(xiàn)顯著的增高。除腦出血后6小時這一時間點外,其余各個時間點的瑞舒伐他汀干預(yù)組(C組)要比腦出血模型組(B組)c-IAP-1陽性細(xì)胞表達增多,Caspase-3,-7陽性細(xì)胞表達減少。瑞舒伐他汀能降低Caspase-3,-7及提高c-IAP-1水平(3)RT-qPCR法顯示,假手術(shù)組組與腦出血模型組和瑞舒伐他汀組組術(shù)后各個時間點相比c-IAP-1及Caspase-3,-7表達水平低。6小時時間點腦出血組和瑞舒伐他汀干預(yù)組相比差異無統(tǒng)計學(xué)意義,其余時間點之間腦出血組和瑞舒伐他汀干預(yù)組相比,c-IAP-1表達降低,Caspase-3,-7表達增高,差異有統(tǒng)計學(xué)意義。結(jié)論:(1)瑞舒伐他汀可以降低腦出血后神經(jīng)功能缺損癥狀。(2)瑞舒伐他汀能夠抑制Caspase-3和Caspase-7活化,進而提高c-IAP-1的表達,c-IAP-1反作用于Caspase-3和Caspase-7,從而進一步抑制Caspase-3和Caspase-7表達。提示瑞舒伐他汀能夠降低由細(xì)胞凋亡影響引起的繼發(fā)性的腦損傷,對神經(jīng)功能起到一定的保護作用。
[Abstract]:Objective: to observe the changes of the expression of Caspase-3, 7(cysteinylaspartate specific proteinases 7caspase-7 and 1(cellular inhibitor of apoptosis proteins 1c-IAP-1 in the perihematoma tissue of rat experimental intracerebral hemorrhage (ICH) induced by recuvastatin, and to observe the expression of 3(cysteinylaspartate specific proteinases 3 Caspase-3, 7(cysteinylaspartate specific proteinases 7caspase-7 and 1(cellular inhibitor of apoptosis proteins 1c-IAP-1.To explore the neuroprotective effect and mechanism of resuvastatin in patients with cerebral hemorrhage.Methods 60 Sprague-Dawley rats were randomly divided into sham-operated group (n = 20), intracerebral hemorrhage group (n = 20) and control group (n = 20).The rats in each group were divided into four time points (6 h, 24 h, 72 h and 5 d), and 5 rats were used at each time point to model and experiment.Establishment of intracerebral hemorrhage model by injecting autologous coagulation blood into right caudate nucleus. (2) Garcia method was used to evaluate the neurological defect of rats at 4 time points after operation.) Immunohistochemical method was used to stain the brain tissue sections around hematoma in three groups of rats.The expression of Caspase-3 + -7 and c-IAP-1 in brain tissue around intracerebral hemorrhage in three groups were detected by RT-PCR at four time points after intracerebral hemorrhage.Results there was no significant difference between the 6 hour cerebral hemorrhage group and the recuvastatin intervention group.At the other three time points, the nerve defect in the control group was significantly lower than that in the cerebral hemorrhage group (P 0.05).The symptoms of nerve defect reached the peak at 72 hours in both groups, and then relieved.Compared with the other two groups at 4 time points after operation, the sham operation group (group A) showed that the nerve defect in group A was lighter and the nerve function was much higher than that in the other two groups.The differences were statistically significant. (2) the expression of c-IAP-1 and Caspase-3 + -7 were observed under microscope in sham-operation group (group A) after intracerebral hemorrhage at four time points after immunohistochemical staining.The positive cells in group B (group B) and group C (group C) were significantly higher than those in group A (sham operation group) at each time point.Except for the time point of 6 hours after intracerebral hemorrhage, the expression of c-IAP-1 positive cells increased and the expression of Caspase-3 + -7 was decreased in the other time points of Risuvastatin intervention group (group C) than that in the model group of intracerebral hemorrhage (group B).Rosuvastatin could decrease Caspase-3 -7 and increase the level of c-IAP-1 by 3RT-qPCR.There was no significant difference in the expression of c-IAP-1 and Caspase-3 ~ (-7) between the sham operation group and the cerebral hemorrhage model group and the rosuvastatin group at different time points after operation compared with those of the cerebral hemorrhage group and the rosuvastatin intervention group.The expression of c-IAP-1 in ICH group and Risuvastatin group was significantly lower than that in control group at other time points.Conclusion Risuvastatin can reduce the neurological deficit symptoms after intracerebral hemorrhage. Resuvastatin can inhibit the activation of Caspase-3 and Caspase-7, and then increase the expression of c-IAP-1. C-IAP-1 counteracts Caspase-3 and Caspase-7, thus further inhibiting the expression of Caspase-3 and Caspase-7.These results suggest that rosuvastatin can reduce the secondary brain injury induced by apoptosis and play a protective role in neurologic function.
【學(xué)位授予單位】：右江民族醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R743.34

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