本文選題：代謝組學(xué)　切入點：超高效液相色譜-四極桿飛行時間質(zhì)譜　出處：《高等學(xué)�；瘜W(xué)學(xué)報》2017年10期

【摘要】：采用基于超高效液相色譜-四極桿飛行時間質(zhì)譜的代謝組學(xué)方法,研究缺血性腦卒中患者和健康人群的血漿,分析了缺血性腦卒中的生物標志物.實驗收集30例缺血性腦卒中患者和17例健康志愿者的血漿樣品,采用主成分分析和正交偏最小二乘判別分析,研究了缺血性腦卒中患者組和健康對照組血漿中的代謝物差異,并進行了代謝通路分析.實驗結(jié)果表明,患者組血漿中的二氫神經(jīng)鞘氨醇、植物鞘氨醇等物質(zhì)含量升高,谷氨酰胺、焦谷氨酸和2-酮丁酸等物質(zhì)含量降低.結(jié)果表明,腦卒中不僅影響了鞘脂類和氨基酸的代謝,還對能量代謝產(chǎn)生了顯著的影響.
[Abstract]:The plasma levels of ischemic stroke patients and healthy people were studied by using the method of metabonomics based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The biomarkers of ischemic stroke were analyzed.Plasma samples were collected from 30 patients with ischemic stroke and 17 healthy volunteers. The differences of plasma metabolites between ischemic stroke patients and healthy controls were studied by principal component analysis and orthogonal partial least squares discriminant analysis.The metabolic pathway was analyzed.The results showed that the contents of dihydroneurinolamine, phytosanine and other substances in plasma were increased, and the contents of glutamine, pyroglutamic acid and 2-ketobutyric acid were decreased in the patients group.The results showed that stroke not only affected the metabolism of sphingolipids and amino acids, but also had a significant effect on energy metabolism.
【作者單位】： 天津醫(yī)科大學(xué)第二醫(yī)院神經(jīng)內(nèi)科;天津中醫(yī)藥大學(xué)中藥學(xué)院;天津大學(xué)精密測試技術(shù)及儀器國家重點實驗室;
【基金】：國家自然科學(xué)基金(批準號:51105274) 天津市自然科學(xué)基金(批準號:16JCYBJC25500) 天津市科學(xué)技術(shù)委員會支撐項目(批準號:12ZCZDSY03100)資助~~
【分類號】：O657.63;R743.3
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本文編號：1704459