本文選題：膠質(zhì)瘤　切入點：microRNA　出處：《山西醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的： 比較原發(fā)性多發(fā)膠質(zhì)瘤和單發(fā)性膠質(zhì)瘤福爾馬林固定石蠟包埋(formalin-fixed and paraffin-embedded, FFPE)組織microRNA表達譜的差異,初步探討顯著差異的microRNA及其靶基因在膠質(zhì)瘤侵襲遷移中可能發(fā)揮的作用。 方法： 收集原發(fā)性多發(fā)膠質(zhì)瘤石蠟包埋組織標(biāo)本6例,同時嚴(yán)格按照匹配條件選取單發(fā)膠質(zhì)瘤6例,常規(guī)石蠟組織切片,提取總RNA并進行質(zhì)量檢測；采用Agilent公司的人類microRNA芯片進行miRNA表達譜的檢測,利用基因芯片分析軟件對芯片結(jié)果進行數(shù)據(jù)分析,篩選出多發(fā)膠質(zhì)瘤和單發(fā)膠質(zhì)瘤具有差異表達的microRNA,再利用生物信息學(xué)分析軟件預(yù)測差異表達InicroRNA的靶基因。 結(jié)果： 所有FFPE組織標(biāo)本中提取的總RNA質(zhì)量檢測均符合質(zhì)控標(biāo)準(zhǔn)。與單發(fā)膠質(zhì)瘤相比,多發(fā)膠質(zhì)瘤中共有128個差異基因有統(tǒng)計學(xué)意義(倍數(shù)2或者0.5),其中有10個microRNAs顯著上調(diào)(倍數(shù)50)：miR-21、miR-455、miR-199、 miR-410、miR-148a、miR-335、miR-34b、miR-664a. miR-542、miR-4453;還有5個表達明顯下調(diào)(倍數(shù)0.05)的InicroRNAs:miR-200a、miR-45、miR-29a、 miR-101、miR-96。基于部分表達差異顯著的microRNAs靶基因預(yù)測結(jié)果,預(yù)測到的靶基因涉及到核酸結(jié)合、蛋白質(zhì)結(jié)合、轉(zhuǎn)移酶活性、上皮間轉(zhuǎn)化等與腫瘤侵襲遷移相關(guān)的多個方面的生物學(xué)功能。 結(jié)論： 1.原發(fā)性多發(fā)膠質(zhì)瘤相比原發(fā)性單發(fā)膠質(zhì)瘤存在]microRNA的差異表達,其中miR-21和miR-200a可能與膠質(zhì)瘤的侵襲和遷移密切相關(guān),有望通過擴大樣本進一步深入研究從而尋找到膠質(zhì)瘤基因診斷和治療的新靶點； 2. FFPE組織可以滿足后續(xù)microRNA芯片等實驗研究,從而為microRNA生物學(xué)功能的研究和探索提供了豐富的資源。
[Abstract]:Objective:. To compare the expression profiles of formalin-fixed formalin-fixed and paraffin-embedded (FFP) in primary multiple gliomas and single gliomas, and to explore the role of microRNA and its target genes in the invasion and migration of gliomas. Methods:. Paraffin-embedded tissue specimens of primary multiple gliomas were collected in 6 cases, and 6 cases of single gliomas were selected strictly according to matching conditions. Routine paraffin sections were used to extract total RNA and detect the quality. The human microRNA chip of Agilent company was used to detect the miRNA expression profile, and the gene chip analysis software was used to analyze the data of the chip. The differentially expressed microRNAs of multiple gliomas and single gliomas were screened and the target genes of differentially expressed InicroRNA were predicted by bioinformatics software. Results:. The quality of total RNA extracted from all FFPE tissue samples was in accordance with the quality control standard. There were 128 differentially expressed genes in multiple gliomas (multiple 2 or 0.5), of which 10 microRNAs were significantly up-regulated (multiple 50: miR-21 miR-455miR-199,miR-410miR-148amiR-338a.miR-542miR-664a. miR-542miR-4453.The other 5 microNASVF miR-200amiR-45miR-29a, miR-101miR-96. MicroRNAs target gene prediction results, The predicted target genes are involved in many biological functions related to tumor invasion and migration, such as nucleic acid binding, protein binding, transferase activity and interepithelial transformation. Conclusion:. 1. The differential expression of microRNA was found in primary multiple gliomas as compared with primary single gliomas, in which miR-21 and miR-200a may be closely related to the invasion and migration of gliomas. It is expected to find a new target for gene diagnosis and treatment of glioma by expanding the sample to further study. 2. FFPE tissue can satisfy the following experimental research such as microRNA chip, which provides abundant resources for the study and exploration of microRNA biological function.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R739.41

【參考文獻】

相關(guān)期刊論文 前2條

1 ;Multiple Gliomas[J];Chinese Journal of Clinical Oncology;2007年06期

2 金晶;周義成;;腦多發(fā)膠質(zhì)瘤影像與病理對照研究[J];中國醫(yī)學(xué)影像學(xué)雜志;2012年02期

，

本文編號：1628002