本文選題：miR-　切入點(diǎn)：膠質(zhì)母細(xì)胞瘤　出處：《華中科技大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年06期 　論文類型：期刊論文

【摘要】：目的研究轉(zhuǎn)染miR-186對(duì)膠質(zhì)母細(xì)胞瘤細(xì)胞增殖能力的影響并探討作用機(jī)制。方法采用熒光定量PCR檢測miR-186在膠質(zhì)母細(xì)胞瘤細(xì)胞系U251、U87-MG及正常星形膠質(zhì)細(xì)胞系HA1800中的表達(dá)水平,將U251細(xì)胞分為miR-186模擬物組和對(duì)照組,分別轉(zhuǎn)染miR-186模擬物和陰性隨機(jī)對(duì)照序列,并用熒光定量PCR檢測miR-186在兩組中的表達(dá)量。采用CKK-8法檢測兩組細(xì)胞細(xì)胞增殖能力,流式細(xì)胞儀測定兩組細(xì)胞凋亡率,Western blot分析Caspase-3、Caspase-8、Caspase-9及Cyclin D1蛋白的表達(dá)。結(jié)果 miR-186低表達(dá)于膠質(zhì)母細(xì)胞瘤細(xì)胞系U251及U87-MG,高表達(dá)于正常星形膠質(zhì)細(xì)胞系HA1800。轉(zhuǎn)染24、48、72及96h后,miR-186模擬物組細(xì)胞增殖(A450nm值)低于對(duì)照組(均P0.05)。轉(zhuǎn)染48h后,miR-186模擬物組凋亡率高于對(duì)照組;miR-186模擬物組Caspase-3、9的表達(dá)量高于對(duì)照組(均P0.01),Caspase-8與對(duì)照組相比差異無統(tǒng)計(jì)學(xué)意義(P0.05)。miR-186模擬物組Cyclin D1表達(dá)水平較對(duì)照組明顯降低(P0.01)。結(jié)論miR-186抑制膠質(zhì)母細(xì)胞瘤細(xì)胞增殖能力,并誘導(dǎo)凋亡,其機(jī)制可能與上調(diào)Caspase-3、9,下調(diào)Cyclin D1有關(guān)。
[Abstract]:Objective to investigate the effect of miR-186 transfection on the proliferation of glioblastoma cells and its mechanism. Methods the expression of miR-186 in glioblastoma cell line U251 U87-MG and normal astrocytoma cell line HA1800 was detected by fluorescence quantitative PCR. U251 cells were divided into miR-186 mimics group and control group. MiR-186 mimics and negative random control sequences were transfected into U251 cells respectively. The expression of miR-186 in the two groups was detected by fluorescence quantitative PCR. The proliferation ability of the two groups was detected by CKK-8 assay. The expression of Caspase-3, Caspase-8, Caspase-9 and Cyclin D1 protein was detected by flow cytometry. Results the expression of miR-186 was low in glioblastoma cell lines U251 and U87-MG, and highly expressed in normal astrocytoma cell line HA1800.After transfection, the miR-186 model was obtained. After 48 hours of transfection, the apoptotic rate of the mimetic group was higher than that of the control group. The expression of Caspase-3O9 in the control group was higher than that in the control group (P 0.01). There was no significant difference in the expression of Caspase-8 between the control group and the control group (P0.05.miR-186). The expression of Cyclin D1 in the control group was significantly lower than that in the control group. Conclusion miR-186 can inhibit the proliferation of glioblastoma cells. The mechanism of apoptosis may be related to the up-regulation of Caspase-3 and down-regulation of Cyclin D1.
【作者單位】： 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬武漢中心醫(yī)院麻醉科;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬武漢中心醫(yī)院急診創(chuàng)傷外科;
【分類號(hào)】：R739.41
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本文編號(hào)：1627995