本文選題：TLR4基因　切入點(diǎn)：大動(dòng)脈粥樣硬化型腦梗死　出處：《青島大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:探討中國(guó)青島地區(qū)漢族人群中TLR4基因rs1927911、rs1927914及rs2149356三個(gè)單核苷酸多態(tài)性位點(diǎn)(single nucleotide polymorphism,SNP)與大動(dòng)脈粥樣硬化型(large-artery atherosclerosis,LAA)腦梗死的相關(guān)性。方法:研究對(duì)象為在青島大學(xué)醫(yī)學(xué)院第二附屬醫(yī)院神經(jīng)內(nèi)科就診的缺血性腦梗死患者和同期在我院體檢中心查體健康者。按照TOAST分型選出105例LAA型腦梗死患者作為L(zhǎng)AA組,以及128例查體健康者作為對(duì)照組。所有受試者入院后第二天清晨空腹抽取前臂靜脈血3ml,用于相關(guān)生化指標(biāo)的檢測(cè)及白細(xì)胞DNA的提取。采集所有受試者的性別、年齡、吸煙史、飲酒史、高血壓病史、糖尿病病史及冠心病病史等基本信息。收集并記錄數(shù)據(jù)。用聚合酶鏈?zhǔn)椒磻?yīng)-限制性片段長(zhǎng)度多態(tài)性(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,PCR-RFLP)檢測(cè)TLR4基因中rs1927911和rs1927914兩個(gè)位點(diǎn)的多態(tài)性,用直接測(cè)序法檢測(cè)TLR4基因中rs2149356位點(diǎn)的多態(tài)性。應(yīng)用SPSS17.0軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。兩組間計(jì)量資料比較采用t檢驗(yàn),符合正態(tài)分布的計(jì)量資料用均數(shù)±標(biāo)準(zhǔn)差(?)表示;計(jì)數(shù)資料應(yīng)用X~2檢驗(yàn);各影響因素與腦梗死相關(guān)性采用多元Logistic回歸分析,p0.05差異有統(tǒng)計(jì)學(xué)意義;用Hardy-Weinberg平衡檢驗(yàn)樣本群體代表性,p0.05具有群體代表性。結(jié)果:rs1927911位點(diǎn)基因型頻率在LAA組與對(duì)照組間的差異有統(tǒng)計(jì)學(xué)意義(p=0.041),rs1927911位點(diǎn)等位基因頻率在LAA組與對(duì)照組間的差異沒(méi)有統(tǒng)計(jì)學(xué)意義(p=0.754),rs2149356位點(diǎn)基因型頻率及等位基因頻率在LAA組與對(duì)照組間的差異有統(tǒng)計(jì)學(xué)意義(p=0.002,p=0.006),rs1927914位點(diǎn)基因型頻率及等位基因頻率在兩組間的差異無(wú)統(tǒng)計(jì)學(xué)意義(p=0.472,p=0.956)。結(jié)論:TLR4基因rs1927911與rs2149356位點(diǎn)多態(tài)性與中國(guó)青島地區(qū)漢族人群大動(dòng)脈粥樣硬化型腦梗死具有相關(guān)性,而rs1927914位點(diǎn)多態(tài)性與LAA型腦梗死未發(fā)現(xiàn)有相關(guān)性。rs2149356位點(diǎn)的等位基因A可能是LAA型腦梗死的易感基因。
[Abstract]:Objective: To investigate the TLR4 gene in Qingdao Han population China rs1927911, rs1927914 and rs2149356 three single nucleotide polymorphisms (single nucleotide, polymorphism, SNP) and large artery atherosclerosis (large-artery atherosclerosis, LAA) cerebral infarction correlation. Methods: the research object in the neurology clinic of the Second Affiliated Hospital of Qiingdao University School of medicine in ischemic cerebral infarction patients and the same period in our hospital health examination center. According to the classification of TOAST type LAA in 105 cases of patients with cerebral infarction were selected as LAA group, and 128 healthy subjects as control group. All subjects second days after admission, fasting forearm venous blood 3ml, for detecting the biochemical indexes of WBC and DNA collect all subjects of sex, age, smoking history, drinking history, history of hypertension, diabetes and coronary heart disease history. Information. Collect and record data. Using polymerase chain reaction restriction fragment length polymorphism (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, PCR-RFLP) to detect the polymorphism of rs1927911 and rs1927914 of TLR4 gene in two loci, the polymorphism of TLR4 gene was detected in rs2149356 locus by direct sequencing. SPSS17.0 software was used for statistical analysis. Two measurement data between groups were compared by t test, with normal distribution measurement data with standard deviation (?); count data using X~2 test; correlation between influence factors and cerebral infarction by using multivariate Logistic regression analysis, there were significant differences in P0.05; with Hardy-Weinberg balance test sample representative group, P0.05 group has representative. Results: there were statistically significant differences of genotype rs1927911 in LAA between the two groups (p= 0.041), rs1927911 The differences in allele frequency between LAA group and control group had no statistical significance (p=0.754), there was a significant difference in rs2149356 genotype and allele frequencies in the LAA group and the control groups (p=0.002, p=0.006), there were no significant differences in rs1927914 genotype and allele frequencies in the between the two groups (p=0.472, p=0.956). Conclusion: there is correlation between type TLR4 atherosclerosis gene rs1927911 and rs2149356 polymorphism and cerebral infarction in Chinese Han population in Qingdao Chinese, and rs1927914 polymorphism and cerebral infarction in type LAA was found between.Rs2149356 allele A may be a susceptible gene of type LAA of cerebral infarction.

【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R743.33

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