本文關鍵詞： 擴散磁共振成像 腦缺血 褪黑素　出處：《中國醫(yī)學影像技術》2017年09期 　論文類型：期刊論文

【摘要】：目的基于大腦中動脈栓塞(MCAO)模型,探討褪黑素(MT)對局灶性腦缺血大鼠腦DWI及腦組織中Fas、FasL、活性Caspase-3蛋白表達的影響。方法將80只SD大鼠隨機分為Sham組(n=16)、MCAO組(n=32)及MT組(n=32)。MCAO組及MT組建立大鼠MCAO模型后,于腦缺血再灌注(IR)前后30min,分別腹腔注射生理鹽水與MT;Sham組進行假手術操作,腹腔注射生理鹽水。對各組大鼠進行行為學評分,于MCAO組及MT組中選取評分為1~3分者納入研究。分別于假手術或IR后6h、24h、72h、7天,行大鼠腦DWI掃描,計算相對DWI信號強度(rDWI-SI);并于每一時間點各組隨機選取大鼠進行Fas、FasL及活性Caspase-3免疫組化染色觀察,并比較各組Fas、FasL、活性Caspase-3陽性細胞數(shù)(個/mm2)。結果最終Sham組16只、MCAO組29只、MT組30只大鼠納入研究。3組間大鼠行為學評分差異具有統(tǒng)計學意義(χ2=50.125,P0.01),MT組及MCAO組行為學評分均高于Sham組(P均0.01),MT組行為學評分低于MCAO組(P0.01)。IR后6h、24h、72h、7天,MCAO組及MT組rDWI-SI值均高于Sham組假手術后相應時間rDWI-SI值(P均0.01);IR后6h、24h、72h,MCAO組rDWI-SI值均高于MT組(P均0.05);IR后7天MT組與MCAO組間rDWI-SI值差異無統(tǒng)計學意義(P0.05)。IR后6h、24h、72h,MT組及MCAO組Fas、FasL染色及活性Caspase-3染色陽性細胞數(shù)均較Sham組增多(P均0.01),MT組較MCAO組減少(P0.05);IR后7天,3組間陽性細胞數(shù)差異無統(tǒng)計學意義(P0.05)。結論在大鼠局灶性腦缺血損傷模型中,MT可有效減低rDWI-SI值,并可抑制Fas、FasL、活性Caspase-3蛋白表達。
[Abstract]:Objective to study the MCAO model of middle cerebral artery embolization (MCAO). To investigate the effects of melatonin (MT) on the expression of Fas-FasLand active Caspase-3 protein in DWI and brain tissue of rats with focal cerebral ischemia, 80 SD rats were randomly divided into Sham group (n = 16), MT group (n = 32), MT group (n = 32) and MT group (n = 10). Thirty minutes before and after cerebral ischemia-reperfusion (IRI), rats in each group were treated with sham operation and intraperitoneal injection of normal saline, respectively. In the MCAO group and MT group, the patients with a score of 1 ~ 3 were selected to be included in the study. The rats were scanned with DWI at 24 h or 72 h after sham-operation or IR for 7 days, respectively. The relative DWI signal intensity of rDWI-SII was calculated, and the Fas-FasL and active Caspase-3 immunohistochemical staining were observed in each group of rats at each time point. The number of Fas-FasL and active Caspase-3 positive cells in each group were compared. Results the behavioral scores of Sham group and MCAO group were significantly higher than those of control group (蠂 ~ 2 = 50.125) and MCAO group (n = 30). The ethology scores in the Sham group were lower than those in the MCAO group (P 0.01) and the rDWI-SI values in the MT group were significantly higher than those in the Sham group at the corresponding time after sham-operation (P < 0.01) and the rDWI-SI value in the MCAO group was significantly higher than that in the Sham group at 7 days after 7 days after IR (P < 0.01) and the rDWI-SI value in the MCAO group was significantly higher than that in the control group (P < 0.05) and the rDWI-SI value difference between the MT group and the MCAO group at 7 days after IR. There was no significant difference in the number of FasL staining and active Caspase-3 staining between the MT group and the MCAO group at 6 hours and 24 hours after IR compared with the Sham group (P < 0.01). There was no significant difference in the number of positive cells between the three groups on the 7th day after IR. Conclusion there is no significant difference in the number of positive cells between the three groups. In the model of focal cerebral ischemia injury, MMT can effectively reduce the rDWI-SI value. It also inhibited the expression of FasL and Caspase-3 protein.
【作者單位】： 濰坊醫(yī)學院醫(yī)學影像學系;濰坊醫(yī)學院附屬醫(yī)院影像中心;濰坊醫(yī)學院臨床醫(yī)學院血液科;濰坊醫(yī)學院附屬醫(yī)院眼科;
【基金】：山東省自然科學基金(ZR2014HQ077、ZR2014JL049) 國家自然科學基金(81000268)
【分類號】：R445.2;R743

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