本文關鍵詞：新型Rho激酶抑制劑FSD-C11對EAE的免疫調(diào)節(jié)作用　出處：《免疫學雜志》2016年08期 　論文類型：期刊論文

  更多相關文章： Fasudil衍生物FSD-C EAE 免疫調(diào)節(jié)

【摘要】：目的探討Rho激酶抑制劑Fasudil衍生物FSD-C11治療實驗性自身免疫性腦脊髓炎(EAE)的免疫調(diào)節(jié)機制。方法采用MOG35-55多肽建立C57BL/6小鼠EAE模型,隨機分為FSD-C11組和Saline組,于免疫后第3天起腹腔注射FSD-C11化合物和Saline。免疫后28 d處死小鼠,FACS法檢測脾組織CD4+T細胞亞群,ELISA法檢測外周免疫系統(tǒng)中細胞因子的分泌情況,Western blot法測定脊髓ROCKⅡ、i NOS、Arg-1、TLR-2和TLR-4的蛋白表達。結果 FSD-C11干預EAE能夠抑制脊髓中ROCKⅡ表達,減少外周CD4+IFN-γ+T細胞,增加CD4+IL-10+和CD4+CD25+T細胞(P0.05),減少外周免疫系統(tǒng)炎性細胞因子IFN-γ、IL-17、IL-6、IL-1β和TNF-α的含量(P0.05),而增加IL-10的含量(P0.05),抑制脊髓組織中巨噬細胞標志蛋白i NOS表達、增加Arg-1的表達(P0.05)。抑制脊髓組織中TLR-2和TLR-4蛋白表達(P0.05)。結論 FSD-C11可調(diào)節(jié)外周免疫細胞活化和增殖,抑制外周免疫系統(tǒng)分泌炎性因子,增加保護性的細胞因子,改善炎性微環(huán)境,促進M1型巨噬細胞向M2型轉(zhuǎn)化,控制CNS的炎性細胞侵潤,從而達到減輕或改善EAE的臨床癥狀。
[Abstract]:Objective to investigate the effect of Rho kinase inhibitor Fasudil derivative FSD-C11 on experimental autoimmune encephalomyelitis. Methods C57BL / 6 mouse EAE model was established by using MOG35-55 peptide. The mice were randomly divided into FSD-C11 group and Saline group. The mice were killed 28 days after immunization by intraperitoneal injection of FSD-C11 compound and Saline.28 days after immunization. The secretion of cytokines in peripheral immune system was detected by Elisa and ROCK 鈪，

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