本文關(guān)鍵詞：氯喹通過抑制自噬促進(jìn)TRAIL誘導(dǎo)的膠質(zhì)瘤細(xì)胞凋亡　出處：《中國臨床神經(jīng)外科雜志》2016年04期 　論文類型：期刊論文

  更多相關(guān)文章： 膠質(zhì)瘤 U細(xì)胞 腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體 氯喹 細(xì)胞凋亡 細(xì)胞自噬

【摘要】：目的探討氯喹對腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(TRAIL)誘導(dǎo)的膠質(zhì)瘤細(xì)胞凋亡的影響。方法體外培養(yǎng)人腦膠質(zhì)瘤細(xì)胞U251和Hela細(xì)胞,分為對照組、氯喹組、重組人TRAIL蛋白(rhTRAIL)組和聯(lián)用組(氯喹與rhTRAIL聯(lián)合應(yīng)用);MTT法檢測細(xì)胞活力,Annexin V-FITC/PI凋亡檢測試劑盒檢測細(xì)胞凋亡,共聚焦顯微鏡檢測GFP-LC3的分布判斷細(xì)胞自噬水平,Western Blot檢測cleaved caspase-8的表達(dá)水平。結(jié)果氯喹和rhTRAIL均顯著增加U251細(xì)胞和Hela細(xì)胞自噬水平,而且聯(lián)用較單獨(dú)應(yīng)用自噬水平更高,Hela細(xì)胞自噬水平明顯高于U251細(xì)胞。U251細(xì)胞和Hela細(xì)胞增殖抑制率和細(xì)胞凋亡率:氯喹組與對照組均無統(tǒng)計學(xué)差異(P0.05),rhTRAIL和聯(lián)用組均明顯高于氯喹組(P0.05),聯(lián)用組明顯高于rhTRAIL組(P0.05)。氯喹組和對照組Cleaved caspases-8水平無統(tǒng)計學(xué)差異(P0.05),rhTRAIL和聯(lián)用組均明顯增高(P0.05),聯(lián)用組明顯高于rhTRAIL組(P0.05)。結(jié)論氯喹能通過抑制細(xì)胞自噬,增強(qiáng)TRAIL誘導(dǎo)的U251細(xì)胞凋亡。
[Abstract]:Objective to investigate the effect of chloroquine on the apoptosis of glioma cells induced by tumor necrosis factor related apoptosis inducing ligand (TRAIL). Cultured human glioma cells U251 and Hela cells in vitro, divided into control group, chloroquine group, recombinant human TRAIL protein (rhTRAIL) group and combination group (combination of chloroquine and rhTRAIL); MTT assay for cell viability, apoptosis detection Annexin V-FITC/PI apoptosis detection kit, total distribution of detection of GFP-LC3 confocal microscopy to determine the level of autophagy and the expression level of Western Blot detection of cleaved caspase-8. Results chloroquine and rhTRAIL significantly increased the autophagy level of U251 cells and Hela cells, and the level of autophagy was higher than that of autophagy alone. The autophagy level of Hela cells was significantly higher than that of U251 cells. U251 cell and Hela cell proliferation inhibition rate and apoptosis rate: there was no significant difference between chloroquine group and control group (P0.05), rhTRAIL and combination group were significantly higher than those in chloroquine group (P0.05), and the combination group was significantly higher than that in rhTRAIL group (P0.05). There was no significant difference in Cleaved caspases-8 level between chloroquine group and control group (P0.05), rhTRAIL and combined group were significantly higher (P0.05), and the combined group was significantly higher than that of rhTRAIL group (P0.05). Conclusion chloroquine can enhance the apoptosis of U251 cells induced by TRAIL by inhibiting autophagy.
【作者單位】： 武漢大學(xué)中南醫(yī)院神經(jīng)外科;十堰市太和醫(yī)院神經(jīng)外科;
【分類號】：R739.4
【正文快照】： 腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(tumor necro-sis factor-related apoptosis-inducing ligand,TRAIL)可誘導(dǎo)細(xì)胞凋亡[1]。但是,隨著研究的深入,TRAIL抵抗的出現(xiàn)成為TRAIL臨床應(yīng)用的障礙。研究發(fā)現(xiàn)TRAIL抵抗的腫瘤細(xì)胞株自噬水平明顯高于TRAIL敏感的腫瘤細(xì)胞株[2]。凋亡和自噬密

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