發(fā)布時(shí)間：2018-08-03 18:25

【摘要】：【目的】肥胖癥是一種慢性代謝類疾病,具有較高的發(fā)病率和高危后果。研究表明,n-3多不飽和脂肪酸(n-3 Polyunsaturated fatty acids,n-3 PUFAs),特別是二十二碳六烯酸(Docosahexaenoic acid,DHA)對(duì)與肥胖癥相關(guān)疾病有較好的防治效果,對(duì)體內(nèi)脂質(zhì)代謝有重要的調(diào)節(jié)作用。探討裂殖壺藻(Schizochytrium sp.)藻油DHA對(duì)高脂飲食誘導(dǎo)肥胖小鼠體重、脂肪組織重量、血脂、肝和脂肪組織病理形態(tài)和脂質(zhì)代謝相關(guān)基因表達(dá)的影響。【方法】通過高脂飲食建立小鼠肥胖模型,以體重增幅15%為標(biāo)準(zhǔn)分出肥胖小鼠。試驗(yàn)共分五組:(1)低脂對(duì)照組;(2)高脂模型組;(3)高脂+低劑量藻油組(50 mg DHA/kg);(4)高脂+中劑量藻油組(100 mg DHA/kg);(5)高脂+高劑量藻油組(200 mg DHA/kg)。其中,藻油處理組灌服相應(yīng)劑量藻油,低脂對(duì)照組和高脂模型組灌胃同等體積玉米油。處理9周后,腹腔麻醉,摘眼球取血并分離血清,測血清中甘油三酯、膽固醇和高密度脂蛋白含量;之后處死小鼠,分離附睪、腎周和腸系膜脂肪組織及肝臟,稱濕重;附睪脂肪和肝組織切片進(jìn)行HE染色,觀察病理變化情況;利用RT-PCR檢測附睪脂肪組織中激素敏感脂酶(Hormone sensitive lipase,HSL)基因的m RNA表達(dá)情況�！窘Y(jié)果】藻油處理組小鼠體重沒有顯著下降,但是腹部脂肪重量顯著降低、脂肪細(xì)胞體積明顯小于高脂模型組;同時(shí)血清中甘油三酯、膽固醇含量顯著降低,肝組織異位脂肪堆積明顯減少;脂肪組織中HSL基因的表達(dá)水平顯著提高�！窘Y(jié)論】裂殖壺藻藻油DHA處理能顯著降低高脂飲食導(dǎo)致的小鼠腹部脂肪積累并改善血脂,可能有利于肥胖癥的防治。
[Abstract]:[Objective] obesity is a chronic metabolic disease with high incidence and high risk consequences. Studies have shown that n-3 polyunsaturated fatty acids (n-3 Polyunsaturated fatty acids, n-3 PUFAs), especially twenty-two carbon six enoic acid (Docosahexaenoic acid, DHA), have good control effect on obesity related diseases and liposomes in vivo. The effects of Schizochytrium sp. on the body weight, fat tissue weight, lipid, liver and fatty tissue, and the expression of lipid metabolism related genes in obese mice induced by high fat diet were investigated. [Methods] the obesity model of mice was established by high fat diet, with 15% weight gain as the standard score. The obese mice were divided into five groups: (1) low fat control group; (2) high fat model group; (3) high fat + low dose algal oil group (50 mg DHA/kg); (4) high fat + medium dose algal oil group (100 mg DHA/kg); (5) high fat + high dose algal oil group (200 mg DHA/kg). After 9 weeks of treatment, the corn oil was treated with intraperitoneal anesthesia, picked up the blood and separated the serum, and measured the content of triglyceride, cholesterol and high density lipoprotein in the serum, then killed the mice, separated epididymis, perirenal and mesenteric adipose tissue and liver, and called wet weight; epididymal fat and liver tissue section were stained with HE to observe the pathological changes; use RT-PCR The expression of M RNA in the Hormone sensitive lipase (HSL) gene in the epididymal adipose tissue was detected. [results] the weight of the mice in the algal oil treatment group was not significantly decreased, but the weight of the abdominal fat decreased significantly and the fat cell volume was significantly smaller than the high fat model group, and the serum triglyceride and cholesterol content decreased significantly. The accumulation of ectopic fat in liver tissue was significantly reduced, and the expression level of HSL gene in adipose tissue was significantly increased. [Conclusion] DHA treatment of alga alga oil can significantly reduce fat accumulation in abdominal fat induced by high fat diet and improve blood lipid, which may be beneficial to the prevention and treatment of obesity.
【作者單位】： 山東省農(nóng)業(yè)科學(xué)院山東省農(nóng)作物種質(zhì)資源中心;山東省農(nóng)業(yè)科學(xué)院生物技術(shù)研究中心;山東大學(xué)生命科學(xué)學(xué)院;
【基金】：山東省農(nóng)業(yè)科學(xué)院青年科研基金(No.2015YQN32) 山東省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)資金(No.SDAIT-26-09) 國家國際科技合作專項(xiàng)項(xiàng)目(No.2012DFA30450)~~
【分類號(hào)】：R589.2

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