發(fā)布時間：2019-06-17 17:10

【摘要】：目的:為了解重慶地區(qū)弓形蟲感染的流行情況及其危險因素,并從基因水平探討人類組織相容性抗原系統(tǒng) HLA-DQ 編碼基因和弓形蟲易感性之間的關(guān)聯(lián),為闡明弓形蟲病的發(fā)病機(jī)制和弓形蟲感染的預(yù)防控制措施提供理論基礎(chǔ)。 方法:采用斷面研究方法,對重慶市主城區(qū)某兩個社區(qū)的一般人群和寵物(貓狗)飼養(yǎng)者、某兩個農(nóng)貿(mào)市場的肉類加工、銷售人員以及近郊某中、小學(xué)家養(yǎng)寵物(貓狗)和未養(yǎng)寵物的初中生和小學(xué)生進(jìn)行了流行病學(xué)調(diào)查(包括問卷調(diào)查,弓形蟲抗體 IgG、IgM、乙肝表面抗原的檢測等)。在篩選出的弓形蟲感染者中,以年齡、性別、民族、職業(yè)人群的工齡、飼養(yǎng)動物的種類和年限為配對條件選取對照組,采用序列特異性引物聚合酶聯(lián)反應(yīng)(PCR/SSP)方法,檢測感染組和對照組 HLA-DQ1 和 DQ3 基因的分布頻率,分析其與弓形蟲感染的相關(guān)性。 結(jié)果:(1)調(diào)查的重慶市某兩個社區(qū),某中、小學(xué)和某兩個農(nóng)貿(mào)市場的人群,弓形蟲總的感染率為 18.30% ,未發(fā)現(xiàn) IgM 陽性者,一般人群的感染率為 11.11%,為全國平均感染率(5.2%)的 2.14 倍,處于國內(nèi)較高水平,男女感染率分別為 15.45%和 20.80%。(2)寵物飼養(yǎng)者的弓形蟲感染率為 23.89%,顯著高于一般人群的感染率,尤其是成人的感染率達(dá)到了34.15%,與一般人群的感染率有顯著差異。(3)寵物飲食以生食為主的飼
[Abstract]:Objective: To study the prevalence and risk factors of Toxoplasma gondii infection in Chongqing, and to study the association between the HLA-DQ coding gene and the susceptibility of Toxoplasma gondii from the gene level. In order to clarify the pathogenesis of toxoplasmosis and the prevention and control measures of toxoplasmosis, a theoretical basis is provided. Methods: The general population and pet (cat dog) breeders in one of the two communities in the main urban area of Chongqing and the meat processing and sales of a certain two farmers' market were adopted. Epidemiological investigation (including questionnaire, Toxoplasma antibody IgG, IgM, and hepatitis B table) was conducted among the personnel and middle school students and primary school students in some middle school, primary school and non-raised pets (including the questionnaire, Toxoplasma antibody IgG, IgM, and hepatitis B). the detection of the surface antigen, etc.), in the screened toxoplasmosis infected persons, the control group is selected according to the age, the sex, the nationality, the working length of the occupational group, the type and the service life of the feeding animal, and the sequence-specific primer polymerase chain reaction (PC) is adopted R/ SSP method for detecting the distribution frequency of HLA-DQ1 and DQ3 genes in the infection group and the control group, and analyzing the distribution frequency of the HLA-DQ1 and DQ3 genes in the infection group and the control group, Results: (1) The total infection rate of Toxoplasma gondii was 18.30% in one of the two communities, one middle school, one primary school and one of the two agricultural markets. The infection rate of the general population was 11.11%, which was the whole country. 2.14-fold of the average infection rate (5.2%), at a higher level in the country, with both male and female infections (2) The infection rate of Toxoplasma gondii was 23.89%, which was significantly higher than that of the general population, especially the infection rate of adults reached 34.1. 5%, the rate of infection with the general population is significant
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R181.3

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