【摘要】： 血吸蟲(chóng)病是世界上僅次于瘧疾的第二大寄生蟲(chóng)病。釘螺是血吸蟲(chóng)的唯一中間宿主,消滅釘螺能有效控制血吸蟲(chóng)病的傳播。Nic是常用的化學(xué)滅螺藥物,但顯效慢、價(jià)格高、對(duì)魚(yú)毒性大。植物滅螺劑因有低毒、易生物降解、價(jià)廉等技術(shù)經(jīng)濟(jì)優(yōu)勢(shì)而受到關(guān)注。本文研究了新型植物滅螺劑(產(chǎn)品)活性成分的定量及作用機(jī)理,主要結(jié)果如下: 1.建立了HPLC法定量分析產(chǎn)品有效成分SX-Ⅰ的方法。方法:色譜柱,C185μm 4.6×150 mm;流動(dòng)相,MeOH- ACN-NH_4AC(V/V/V,30:60:10);流速,1.0mL/min;檢測(cè),UV210 nm。結(jié)果:SX-Ⅰ線性范圍為100～500μg/mL,平均回收率108.8%,變異系數(shù)(RSD)0.14%。結(jié)論:該方法簡(jiǎn)捷、準(zhǔn)確,可用于該產(chǎn)品有效成分SX-Ⅰ的質(zhì)量控制。 2.研究了NM和SX-Ⅰ滅螺藥物對(duì)釘螺酶活性及蛋白的誘導(dǎo)作用。方法:HPLC檢測(cè)及PAGE和SDS-PAGE電泳分析。結(jié)果:釘螺AchE、GOT的活性隨浸泡時(shí)間延長(zhǎng),先增強(qiáng)后減弱;而釘螺的總的酶帶數(shù)也出現(xiàn)先增加后減少的現(xiàn)象。結(jié)論:NM和SX-Ⅰ溶液滅殺釘螺,使其正常的物質(zhì)代謝平衡被破壞,蛋白合成受阻,導(dǎo)致釘螺中毒死亡。 3.測(cè)定了不同濃度SX果實(shí)粉、AC種粉及CHSO_4復(fù)配對(duì)釘螺AchE活性的影響。方法:改進(jìn)的Ellman法測(cè)定AchE對(duì)藥物的靈敏度,采用L_(25)(5~3)正交試驗(yàn)和極差分析等方法,試驗(yàn)確定3因素及其5水平,測(cè)定酶活性抑制率最高的條件組合。結(jié)果:滅螺藥物復(fù)配對(duì)釘螺AchE活性抑制率的最佳組合為:SX果實(shí)粉25mg/L、AC種粉16 mg/L、CUSO_42 mg/L。結(jié)論:該方法簡(jiǎn)單、有效,可用于研發(fā)對(duì)釘螺高毒、魚(yú)低毒的新型滅螺劑提供參考。
[Abstract]:Schistosomiasis is the second largest parasitic disease in the world after malaria. Oncomelania hupensis is the only intermediate host of Schistosoma japonicum. The elimination of Oncomelania hupensis can effectively control the spread of schistosomiasis. Nic is a common chemical snail killer, but it has a slow effect, high price and high toxicity to fish. Plant molluscicides have attracted much attention because of their low toxicity, biodegradability, low price and other technical and economic advantages. In this paper, the quantitative analysis and action mechanism of new plant snail repellent were studied. The main results are as follows: 1. A HPLC method for quantitative analysis of SX- 鈪，

本文編號(hào)：2164479