【摘要】： [目的] 探討醫(yī)院感染頻發(fā)的潛在因素之一細菌對消毒劑的抗性變化情況；對消毒劑抗性檢測方法的試驗過程進行規(guī)范,找出適合對消毒劑抗性進行長期監(jiān)測的試驗方法：通過完善的試驗方法對多耐藥銅綠假單胞菌的抗性進行檢測；通過抗性誘導的方法觀察多耐藥銅綠假單胞菌對消毒劑抗性的變化情況。 [方法] 使用營養(yǎng)肉湯稀釋法進行過氧乙酸、戊二醛、84消毒液、碘伏、新潔爾滅對多耐藥銅綠假單胞菌最小抑菌濃度(MIC)的測定；使用MIC加中和劑法對多耐藥銅綠假單胞菌進行最小殺菌濃度(MBC)的測定；通過遞增濃度誘導法對戊二醛、碘伏、新潔爾滅進行抗性誘導試驗。 [結果] 23株多耐藥銅綠假單胞菌中發(fā)現(xiàn)了對戊二醛(1株)、新潔爾滅(7株)抗性大于標準菌株(比較MIC)的菌株,多耐藥銅綠假單胞菌對不同消毒劑的抗性情況不同。未發(fā)現(xiàn)在常規(guī)使用的消毒劑濃度下不能殺滅的菌株。通過對戊二醛、碘伏、新潔爾滅抗性的誘導,均出現(xiàn)了抗性增強的現(xiàn)象。 [結論] 多耐藥銅綠假單胞菌對常用消毒劑的抗性存在超過標準菌株的情況,由其引起的醫(yī)院感染后果較嚴重,應引起足夠的重視。研究中所選擇的五種常用消毒劑在常規(guī)推薦使用濃度下均可殺滅試驗菌株,提示正確使用消毒劑可以達到預防醫(yī)院感染的目的。長時間接觸亞致死劑量的消毒劑可以誘導對該種消毒劑的抗性增強,提示在日常消毒工作中應注意足量使用消毒劑,不能隨意降低濃度長期使用。
[Abstract]:[objective] to investigate the change of bacterial resistance to disinfectants, one of the potential factors of frequent nosocomial infection, and to standardize the test process of the methods for detecting the resistance of disinfectants. To find out the suitable long-term monitoring method for the resistance of disinfectant: to detect the resistance of multidrug resistant Pseudomonas aeruginosa by perfect test method; The resistance of multidrug resistant Pseudomonas aeruginosa to disinfectant was observed by resistance induction. [methods] the minimal inhibitory concentration (MIC) of peracetic acid, glutaraldehyde (Glutaraldehyde) 84 disinfectant, iodophor and neugeramine against multidrug resistant Pseudomonas aeruginosa was determined by nutrient broth dilution method. The minimal bactericidal concentration (MBC) of multidrug resistant Pseudomonas aeruginosa was determined by MIC plus neutralization method, and the resistance induction test of glutaraldehyde, iodophor and neugericides was carried out by increasing concentration induction method. [results] among 23 strains of multidrug resistant Pseudomonas aeruginosa, glutaraldehyde (1 strain) and ninjelme (7 strains) were found to be more resistant than standard strains (MIC). The resistance of multidrug resistant Pseudomonas aeruginosa to different disinfectants was different. No strains were found that could not be killed at the concentration of conventional disinfectants. The resistance to glutaraldehyde, iodophor and nebulidene were all increased. [conclusion] the resistance of multidrug resistant Pseudomonas aeruginosa to common disinfectants is higher than that of standard strains, and the nosocomial infection caused by it is serious, which should be paid more attention to. The five commonly used disinfectants selected in the study could kill the tested strains at the recommended concentration, indicating that the correct use of disinfectants could prevent nosocomial infection. Long exposure to sublethal dose of disinfectant could induce the resistance of the disinfectant to the disinfectant. It suggested that sufficient amount of disinfectant should be paid attention to in the daily disinfection work, and the concentration of disinfectant should not be reduced at will for a long time.
【學位授予單位】：山東大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R187

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