發(fā)布時(shí)間：2018-05-04 01:21

  本文選題：醫(yī)用臭氧 + 殺菌作用��； 參考：《南方醫(yī)科大學(xué)》2009年碩士論文

【摘要】： 臭氧(O_3)是由三個(gè)氧原子組成的分子,臭氧氣體是一種淡藍(lán)色帶有刺激性酸味的氣體,在空氣中極不穩(wěn)定,在常溫常壓空氣中易還原為氧氣(半衰期40min)。醫(yī)用臭氧(Medical ozone)為醫(yī)用純氧通過臭氧發(fā)生器產(chǎn)生的臭氧與醫(yī)用純氧的混合氣體,臭氧濃度在0.05%～5%之間。與日常生活用和工業(yè)用臭氧不同,醫(yī)用臭氧由醫(yī)用純氧而非空氣生成,故不含氮氧化合物(NO_2)等有毒氣體分子,且要求其濃度能夠被精確調(diào)控,另外由于其極不穩(wěn)定性故必須即時(shí)制備即時(shí)使用(為敘述方便,如無特殊說明下文中的“臭氧”均代指“醫(yī)用臭氧”)。 臭氧具有極強(qiáng)的氧化性,其氧化性僅次于氟和過硫酸鹽。已有大量研究表明,體外在生物耐受劑量濃度下(20～80μg/ml)的臭氧就能有效殺滅或滅活水體中多種微生物,包括G~+或G~-菌、HAV、HIV等。 我國有慢性肝炎病毒包括乙肝病毒(HBV)和丙肝病毒(HCV)感染者約1.5億,主要傳播途徑為血液傳播。由于檢測技術(shù)的限制及病毒感染窗口期的存在,血液制品的生物安全性尚未得到有效解決。降低血液肝炎病毒載量對(duì)移植肝再感染也有利。對(duì)于慢性乙型肝炎病毒感染者進(jìn)行肝移植,目前主要預(yù)防方法為口服核苷類似物聯(lián)合靜脈用乙肝免疫球蛋白(HBIG),費(fèi)用高昂,且HBIG國內(nèi)暫無上市;對(duì)于HCV感染者的移植肝再感染目前尚無有效的預(yù)防方法。自體輸血作為目前最為安全有效的輸血方式雖具有節(jié)約血源、減少不必要的輸血反應(yīng)和感染性疾病傳播等諸多優(yōu)勢,但可能發(fā)生細(xì)菌污染,輸用后將造成嚴(yán)重甚至致命的后果。以上問題的解決均依賴于安全、有效的血液凈化方式。 腹水是肝硬化最常見的并發(fā)癥之一。自發(fā)性腹膜炎(Spontaneous bacterialperitonitis,SBP)在肝硬化病例中的發(fā)生率為10%-25%,在腹水病例中的平均發(fā)生率約30%,是肝病嚴(yán)重的標(biāo)志,病死率很高。SBP主要發(fā)生機(jī)制為宿主免疫防御功能減弱、腸道細(xì)菌移位(BT),感染的病原菌半數(shù)是大腸埃希菌,其次是金黃色葡萄球菌、腸球菌等。如能對(duì)此類患者腹水進(jìn)行有效滅菌處理,其腹水濃縮后亦可回輸體內(nèi),避免蛋白丟失或可提高治療效果。 本研究目的在于利用臭氧體外水介質(zhì)中能有效殺滅多種微生物的特性,試驗(yàn)臭氧在體外對(duì)體液(血漿、腹水等)的殺菌效果,為利用臭氧在體外對(duì)體液進(jìn)行生物凈化的可行性進(jìn)行評(píng)價(jià)并提供實(shí)驗(yàn)參數(shù)。 第一部分醫(yī)用臭氧對(duì)血漿中細(xì)菌殺滅作用的研究 1.1研究方法 向5份10ml含等量(0.5麥?zhǔn)蠞舛?細(xì)菌的生理鹽水中分別通入濃度為5μg/ml、10μg/ml、15μg/ml、20μg/ml、90μg/ml的過濾臭氧作用5min。對(duì)照組為10ml含等量細(xì)菌的生理鹽水,以同樣方法通入等量過濾醫(yī)用純氧。取兩組稀釋液各1ml接種于細(xì)菌培養(yǎng)皿,傾注普通營養(yǎng)瓊脂15～20ml并充分混勻,每組做3個(gè)培養(yǎng)皿重復(fù),37℃培養(yǎng)24h后計(jì)數(shù)兩組菌落數(shù),計(jì)算殺菌率。殺菌率=(對(duì)照組平均菌落數(shù)-試驗(yàn)組平均菌落數(shù))/對(duì)照組平均菌落數(shù)×100%。每個(gè)實(shí)驗(yàn)重復(fù)5次。驗(yàn)證臭氧對(duì)生理鹽水中細(xì)菌的殺滅效果。 取等濃度的大腸埃希菌和金黃色葡萄球菌菌懸液0.5ml加入10ml血漿中。探索高濃度(90μg/ml)的過濾醫(yī)用臭氧分別作用5min、10min和20min對(duì)其殺菌效果。 向無菌生理鹽水中添加人新鮮冰凍血漿,按生理鹽水與血漿體積比29:1、19:1、9:1、4:1、1:1、0:10混合,得到血漿終濃度分別為3.3%、5.0%、10.0%、20.0%、50.0%、100.0%的系列混合物。實(shí)驗(yàn)組于血漿濃度為分別為3.3%、5.0%、10.0%、20.0%、50.0%、100.0%生理鹽水-血漿混合物各10ml中加入含菌量為0.5麥?zhǔn)蠞舛鹊木鷳乙?.5ml,通入80μg/ml過濾臭氧作用不同時(shí)間(5min、10min、20min);對(duì)照組為加入等量細(xì)菌的同稀釋度稀釋血漿,以同樣流速通入等量過濾醫(yī)用純氧。探索血漿對(duì)臭氧殺菌的干擾作用。 1.2研究結(jié)果 20μg/ml以上臭氧作用5min對(duì)10ml生理鹽水中大腸埃希菌、金黃色葡萄球菌殺滅率均可達(dá)100%,如減小臭氧濃度至5μg/ml對(duì)兩種細(xì)菌的殺菌率分別降至46.72%和38.50%。臭氧對(duì)血漿中大腸埃希菌、金黃色葡萄球菌殺滅效果較生理鹽水中差,90μg/ml臭氧作用5min對(duì)10ml血漿中大腸埃希菌、金黃色葡萄球菌的殺菌率分別只有18.14%和17.86%。延長作用時(shí)間可提高殺菌效果,臭氧作用20min對(duì)兩種細(xì)菌的殺菌率可分別提高至50.35%和43.58%。向生理鹽水中添加血漿,隨血漿濃度的升高,不同血漿濃度(3.3%、5.0%、10.0%、20.0%、50.0%、100.0%)經(jīng)濃度為80μg/ml的臭氧作用20min對(duì)大腸埃希菌及金黃色葡萄球菌的殺菌率從100%降低至50%以下。不同血漿濃度(3.3%、5.0%、10.0%、20.0%、50.0%、100.0%)組間對(duì)大腸埃希菌和金黃色葡萄球菌兩種細(xì)菌的殺菌率均存在顯著性差異(F=51420.241,P=0.000和F=75088.996,P=0.000),當(dāng)血漿濃度在10.0%以上時(shí)以80μg/ml臭氧作用20min(相當(dāng)于臭氧總量19.2mg)即可有細(xì)菌存活。不同作用時(shí)間組間對(duì)大腸埃希菌的殺菌率存在顯著性差異(F=30885.690,P=0.000),對(duì)金黃色葡萄球菌亦然(F=27441.387,P=0.000),作用時(shí)間長者殺菌效果較好。 1.3研究結(jié)論 1、臭氧對(duì)生理鹽水中大腸埃希菌和金黃色葡萄球菌有明顯的殺菌效果,20μg/ml以上臭氧作用5min對(duì)10ml生理鹽水中兩種細(xì)菌殺滅率即可達(dá)100%。 2、臭氧對(duì)大腸埃希菌的殺菌效果優(yōu)于金黃色葡萄球菌。 3、向生理鹽水中添加血漿后,臭氧對(duì)其殺菌效率迅速降低。血漿濃度在10.0%以上時(shí)以80μg/ml臭氧作用20min(相當(dāng)于臭氧總量19.2mg)即可有細(xì)菌存活。使用90μg/ml臭氧作用20min對(duì)血漿中的大腸埃希菌和金黃色葡萄球菌的殺滅率僅為50.35%和43.58%。說明血漿中存在抗氧化物質(zhì),能干擾臭氧的殺菌作用。進(jìn)一步延長作用時(shí)間或可獲得更高殺菌率。 第二部分醫(yī)用臭氧對(duì)肝硬化所致腹水中細(xì)菌的殺滅作用研究 2.1研究方法 收集4例肝硬化頑固性腹水患者腹水標(biāo)本共6份(其中一名患者于不同時(shí)間點(diǎn)取腹水3次)。4名患者的腹水按腹水TP含量由小到大的順序編號(hào)1～4同一患者的不同腹水按腹水TP含量由小到大的順序編號(hào)A～C(4號(hào)標(biāo)本與C標(biāo)本為同一份標(biāo)本)。腹水采集前1周內(nèi)未全身或局部使用抗生素。經(jīng)檢驗(yàn)腹水無菌,加入等量的大腸埃希菌和金黃色葡萄球菌,37℃培養(yǎng)24h后觀察不同腹水中培養(yǎng)的菌落數(shù)。每份標(biāo)本做7個(gè)培養(yǎng)皿重復(fù),取其平均值。 取每份腹水標(biāo)本各10ml中加入等濃度菌懸液0.5ml,均通入80μg/ml臭氧分別作用5min、10min、20min,含等量細(xì)菌的同來源腹水作為對(duì)照組以同樣方法通入等量過濾醫(yī)用純氧。取兩組的稀釋液接種細(xì)菌培養(yǎng)皿,傾注普通營養(yǎng)瓊脂后于37℃培養(yǎng)24h,進(jìn)行活菌菌落計(jì)數(shù),計(jì)算殺菌率,觀察80μg/ml臭氧作用不同時(shí)間對(duì)4份不同來源腹水的殺菌效果以及同一病人不同腹水的殺菌效果。 實(shí)驗(yàn)當(dāng)天同時(shí)檢測腹水總蛋白(TP)等腹水常規(guī)及生化指標(biāo),觀察臭氧對(duì)不同腹水的殺菌效果與該腹水TP含量的關(guān)系。 2.2研究結(jié)果 1～4號(hào)標(biāo)本的腹水TP值分別為0.6、2.2、16.6、25.7 g/L。等量細(xì)菌加入4份腹水培養(yǎng)24小時(shí)后菌落數(shù)存在差異,1號(hào)標(biāo)本形成菌落數(shù)最多,2號(hào)大于3號(hào),4號(hào)菌落數(shù)最少。80μg/ml臭氧對(duì)不同來源腹水中大腸埃希菌的殺菌效果存在顯著性差異(F=40499.603,P=0.000),對(duì)不同腹水中金黃色葡萄球菌殺菌率亦存在顯著性差異(F=92525.40,P=0.000),其中80μg/ml臭氧對(duì)1號(hào)腹水標(biāo)本(腹水TP含量為0.6g/L)和2號(hào)標(biāo)本(腹水TP含量為2.0g/L)作用10 min(相當(dāng)于臭氧總量9.6mg)后對(duì)兩種細(xì)菌的殺菌率即可達(dá)100%,而對(duì)3號(hào)和4號(hào)標(biāo)本(腹水TP含量分別為16.6g/L和25.7 g/L)作用10min后對(duì)兩種細(xì)菌殺菌率仍均低于70%。臭氧不同作用時(shí)間(5min、10min、20min)間對(duì)大腸埃希菌的殺菌率存在顯著性差異(F=28514.907,P=0.000),對(duì)金黃色葡萄球菌的殺菌率亦存在顯著性差異(F=13066.314,P=0.000),作用時(shí)間長者殺菌效果較好,作用20min時(shí)對(duì)4份腹水中兩種細(xì)菌的殺菌率均達(dá)到70%以上。不同腹水及不同臭氧作用時(shí)間對(duì)臭氧殺滅大腸埃希菌效率的影響存在交互效應(yīng)(F=9775.527,P=0.000),對(duì)金黃色葡萄球菌的殺菌效果的影響亦存在交互作用(F=3242.915,P=0.00)(表2-3、表2-4)。臭氧作用5 min、10min、20min均顯示80μg/ml的臭氧對(duì)4份腹水的殺菌效率1號(hào)優(yōu)于2號(hào),其次為3號(hào),對(duì)4號(hào)標(biāo)本殺菌效果最差(圖2-3),即不同病人腹水其TP含量高者臭氧對(duì)其殺滅效果較差。 A～C號(hào)標(biāo)本的腹水TP值分別為14.6、22.1、25.7 g/L。80μg/ml臭氧對(duì)同一病人3份不同腹水中大腸埃希菌的殺菌效果存在顯著性差異(F=395.821,P=0.000),對(duì)不同腹水中金黃色葡萄球菌殺菌率亦存在顯著性差異(F=520.915,P=0.000)。不同作用時(shí)間組間對(duì)大腸埃希菌的殺菌率存在顯著性差異(F=38534.299,P=0.000),對(duì)金黃色葡萄球菌的殺菌率亦存在顯著性差異(F=10800.111,P=0.000),作用時(shí)間長者殺菌效果較好。同一病人的不同腹水及不同臭氧作用時(shí)間對(duì)臭氧殺滅大腸埃希菌效率的影響存在交互效應(yīng)(F=227.996,P=0.000),對(duì)金黃色葡萄球菌的殺菌效果的影響亦存在交互作用(F=140.123,P=0.00)(表2-6、表2-7)。臭氧作用5 min、10min、20min均顯示80μg/ml的臭氧對(duì)3份腹水的殺菌效率A標(biāo)本優(yōu)于B標(biāo)本,C標(biāo)本最差(圖2-5)。對(duì)同一病人的腹水來說,腹水中TP含量高者臭氧對(duì)其殺滅效果較差。 2.3研究結(jié)論 1、不同腹水環(huán)境對(duì)細(xì)菌生長有影響,腹水TP較高的標(biāo)本細(xì)菌存活數(shù)量較少。 2、臭氧對(duì)腹水的殺菌效果優(yōu)于血漿,但對(duì)不同腹水殺菌效果存在差異。驗(yàn)證了在蛋白含量較血漿低下的體液如腹水中生物安全劑量范圍(不超過80μg/ml)內(nèi)的臭氧即可發(fā)揮有效殺菌效果(即使對(duì)TP含量達(dá)16.6g/L的腹水以80μg/ml臭氧作用20min其殺菌率亦可達(dá)90%以上),腹水TP含量低者臭氧對(duì)其殺菌效率較高。 3、延長作用時(shí)間可提高臭氧殺菌效率。
[Abstract]:Ozone (O_3) is a molecule consisting of three oxygen atoms. The ozone gas is a light blue and irritating sour gas. It is extremely unstable in the air and is easily reduced to oxygen (half life 40min) in the atmospheric pressure air. Medical ozone (Medical ozone) is a mixture of ozone and medical pure oxygen produced by the ozone generator for medical pure oxygen. The concentration of ozone is between 0.05% and 5%. Unlike daily and industrial ozone, medical ozone is produced from medical pure oxygen instead of air, so it does not contain noxious gas molecules such as nitrogen and oxygen compounds (NO_2) and requires its concentration to be regulated accurately. If there is no special description, ozone is referred to as "medical ozone".
Ozone is highly oxidizable, and its oxidizability is second only to fluorine and persulfate. A large number of studies have shown that in vitro, ozone can effectively kill or inactivate a variety of microorganisms in the water body, including G~+ or G~- bacteria, HAV, HIV, and so on, under the biological tolerance dose concentration (20~80 Mu g/ml).
There are about 150 million of chronic hepatitis viruses, including hepatitis B virus (HBV) and hepatitis C virus (HCV) infection in our country. The main route of transmission is blood transmission. The biological safety of blood products has not been effectively solved because of the restriction of detection technology and the presence of the virus infection window period. The lower blood hepatitis virus load is also beneficial to the reinfection of the transplanted liver. For patients with chronic hepatitis B virus infection, the main prevention method is the combination of oral nucleoside analogues and intravenous hepatitis B immunoglobulin (HBIG). The cost is high, and HBIG is not available at home. There is no effective prevention method for reinfection of liver in HCV infected people. Autologous blood transfusion is the most safe and safe at present. Although effective blood transfusion has many advantages such as saving blood source, reducing unnecessary transfusion reaction and infectious disease transmission, bacterial contamination may result in serious or even fatal consequences. The solutions of the above problems are all dependent on safety and effective blood purification.
Ascites is one of the most common complications of liver cirrhosis. The incidence of Spontaneous bacterialperitonitis (SBP) in the cases of liver cirrhosis is 10%-25%, the average incidence in the cases of ascites is about 30%. It is a sign of serious liver disease, and the high mortality rate of.SBP is that the host immune defense function is weakened and the intestinal bacteria are weakened. BT, half of the infected pathogens are Escherichia coli, followed by Staphylococcus aureus, Enterococcus and so on. If it can effectively sterilize the ascites of such patients, the ascites can also be returned to the body after concentration, to avoid protein loss or to improve the therapeutic effect.
The purpose of this study is to test the bactericidal effect of ozone in body fluid (plasma, ascites, etc.) in vitro by using ozone in water medium, and to evaluate the feasibility of using ozone to purify the body fluid in vitro and provide experimental parameters.
The first part is about the killing effect of medical ozone on bacteria in plasma.
1.1 research methods
The concentration of 5 10ml containing equal (0.5 MF) bacteria was injected into the physiological saline of 5 mu g/ml, 10 mu g/ml, 15 mu g/ml, 20 mu g/ml, 90 u g/ml, 5min. control group was the normal saline containing the equal amount of bacteria in 10ml, and the same method was used to filter the medical pure oxygen in the same amount. The two groups of diluents were inoculated in the bacterial culture dish and poured out. Common nutrient agar was 15 ~ 20ml and well mixed, 3 cultures were repeated in each group. Two groups of colonies were counted and bactericidal rate was counted at 37 C for 24h. Bactericidal rate = (average colony number of the control group - average colony number in test group) / the average colony number of control group was repeated 5 times per experiment. The effect of ozone on bacteria in normal saline was verified.
The concentration of Escherichia coli and Staphylococcus aureus suspension 0.5ml were added to the 10ml plasma. The bactericidal effect of 5min, 10min and 20min on the high concentration (90 u g/ml) of filtered medical ozone was investigated respectively.
A mixture of fresh frozen plasma was added to aseptic saline, and a series of 3.3%, 5%, 10%, 20%, 50%, 100% mixture of 3.3%, 5%, 20%, 50%, 100%, respectively. The plasma concentration of the experimental group was 3.3%, 5%, 10%, 20%, 50%, 100% saline plasma mixture, respectively. Each 10ml was added to the bacterial suspension containing 0.5 malt concentration of bacteria 0.5ml, and the ozone action was filtered through 80 g/ml (5min, 10min, 20min). The control group was diluted with the same dilution of the same amount of bacteria and filtered the medical pure oxygen at the same flow rate, and the interference effect of plasma on ozone sterilization was explored.
1.2 research results
The killing rate of 5min for Escherichia coli and Staphylococcus aureus in 10ml normal saline up to 20 g/ml was 100%, such as reducing the ozone concentration to 5 u g/ml to 46.72% and 38.50%. ozone against Escherichia coli in plasma, the killing effect of Staphylococcus aureus was worse than that in saline, 90 mu g/ml stinky. The bactericidal rate of 5min in 10ml plasma of Escherichia coli and Staphylococcus aureus, respectively, only 18.14% and 17.86%. prolongation time can improve the germicidal efficacy. The bactericidal rate of ozone action 20min on two kinds of bacteria can be increased to 50.35% and 43.58%. to the physiological saline, with the increase of plasma concentration and different plasma concentration. 3.3%, 5%, 10%, 20%, 50%, 100%) the bactericidal rate of 20min to Escherichia coli and Staphylococcus aureus from 100% to 50% below the concentration of 80 g/ml ozone. The bactericidal rates of Escherichia coli and Staphylococcus aureus among the groups of different plasma concentrations (3.3%, 5%, 10%, 20%, 50%, etc.) were all significant The difference (F=51420.241, P=0.000 and F=75088.996, P=0.000), when the plasma concentration is above 10%, the bacteria can survive with the action of 80 mu g/ml (equivalent to the total amount of ozone 19.2mg). The bactericidal rate of Escherichia coli between different time groups is significantly different (F= 30885.690, P=0.000), and to Staphylococcus aureus (F=27441.38). 7, P=0.000), the effect of germicidal effect is better.
1.3 research conclusions
1, ozone has obvious bactericidal effect on Escherichia coli and Staphylococcus aureus in normal saline, and the killing rate of two kinds of bacteria in 10ml normal saline by ozone over 20 g/ml is 100%.
2, ozone has a better germicidal efficacy against Escherichia coli than Staphylococcus aureus.
3, after the plasma was added to the saline, the bactericidal efficiency of ozone decreased rapidly. When the plasma concentration was above 10%, the bacteria could survive with 80 mu g/ml ozone action 20min (equivalent to the total ozone amount 19.2mg). The killing rate of Escherichia coli and Staphylococcus aureus in plasma was only 50.35% and 43.58% with 90 g/ml ozone action 20min. It indicates that there are antioxidants in the plasma, which can interfere with the germicidal efficacy of ozone. Further prolonging the time of action may lead to a higher bactericidal rate.
The second part is about the killing effect of medical ozone on bacteria in ascites caused by cirrhosis.
2.1 research methods
A total of 6 specimens of ascites in 4 patients with cirrhosis of the refractory ascites were collected (one of the patients took ascites at different time points for 3 times). The ascites of the.4 patients were numbered in the order of the TP content of ascites from small to large, and the different ascites of the same patient were numbered A to C in the order of TP in the ascites. (specimens of No. 4 and C specimens were the same specimen). Antibiotics were not used whole-body or local within 1 weeks before water collection. After testing ascitic asepsis, adding the equal amount of Escherichia coli and Staphylococcus aureus, the number of colonies in different ascites was observed after culture of 24h at 37 C. The average value of each specimen was repeated in 7 Petri dishes.
The same concentration bacteria suspension 0.5ml was added to the 10ml of each ascitic specimen. The same amount of ascitic water containing the same amount of bacteria was injected into the same amount of 80 u g/ml ozone to filter medical pure oxygen in the same amount as the control group. Two groups of diluents were inoculated to the bacterial culture dish, and then the common nutrient agar was poured into 24h at 37. The bacteria colony count was counted and the bactericidal rate was calculated. The bactericidal effect of 80 g/ml ozone action on 4 different sources of ascites and the bactericidal effect of different ascites in the same patient were observed.
On the same day, routine and biochemical indexes of ascites, such as ascites total protein (TP), were detected simultaneously. The relationship between the effect of ozone and the TP content of ascites was observed.
2.2 research results
The TP value of the ascites of 1~4 specimens was 0.6,2.2,16.6,25.7 g/L. equal amount of bacteria added to 4 ascites for 24 hours, and the number of colony number was different, the number of colony 1 was the most, 2 greater than 3, and the number of colony number 4 was least.80 mu g/ml ozone on the bactericidal effect of Escherichia coli in the ascites of different sources (F=40499.603, P=0.) 000), the bactericidal rate of Staphylococcus aureus in different ascites was also significantly different (F=92525.40, P=0.000). The bactericidal rate of 80 g/ml ozone on 1 ascites (ascites TP content 0.6g/L) and 2 specimens (TP content of ascites 2.0g/L) was 10 min (equivalent to ozone total 9.6mg), and the bactericidal rate of two bacteria was 100%, and 3 and 4. The bactericidal rate of two bacteria was still lower than that of 70%. ozone (5min, 10min, 20min), and the bactericidal rate of Escherichia coli was significantly different (F=28514.907, P=0.000), and the bactericidal rate of Staphylococcus aureus was also significantly different (F=13066.314, P=). The bactericidal rate of the two bacteria was still lower than that of 70%. ozone (5min, 10min, 20min). The bactericidal rate of the specimens (ascites TP and 25.7 g/L respectively) 0), the germicidal effect of the action time elders was better, the bactericidal rate of two bacteria in the 4 ascites was above 70% when the effect of 20min. The effect of different ascites and different ozone time on the efficiency of ozone killing Escherichia coli (F=9775.527, P=0.000), and the effect on the bactericidal effect of Staphylococcus aureus also existed. Interaction (F=3242.915, P=0.00) (table 2-3, table 2-4). Ozone action 5 min, 10min, 20min showed that 80 micron of ozone bactericidal efficiency for 4 ascites was better than No. 2, followed by No. 3, and the worst bactericidal effect on specimen 4 (Figure 2-3), that is, the ozone of different patients with high TP content in the ascites was less effective.
The TP value of the ascites of A ~ C was 14.6,22.1,25.7 g/L.80 micron g/ml, respectively, for the bactericidal effect of the 3 different ascites of the same patient (F=395.821, P=0.000). The bactericidal rate of Staphylococcus aureus in different ascites was also significantly different (F=520.915, P=0.000). The bactericidal rate of Escherichia coli has significant difference (F=38534.299, P=0.000), the bactericidal rate of Staphylococcus aureus also has significant difference (F=10800.111, P=0.000), and the effect of germicidal effect is better. The influence of different ascites and different ozone action on the efficiency of ozone killing Escherichia coli in the same patient Effect (F=227.996, P=0.000), the effect on the bactericidal effect of Staphylococcus aureus also had interaction (F=140.123, P=0.00) (table 2-6, table 2-7). Ozone action 5 min, 10min, 20min showed that 80 mu of ozone on 3 ascites bactericidal efficiency A specimens were superior to B standard, C specimens (Figure 2-5). For the ascites of the same patient, TP in the ascites. The ozone with high content was less effective in killing it.
2.3 research conclusions
1, different ascites environment had an effect on bacterial growth, and the number of bacteria with higher TP ascites was less.
2, the bactericidal effect of ozone on ascites is better than that of plasma, but there are different bactericidal effects on different ascites. It is proved that the effective bactericidal effect of ozone in the biological safe dose range (not more than 80 g/ml) in the fluid like ascites, which is lower than plasma, can be effective (even with the TP content of 16.6g/L in the ascites with 80 u g/ml ozone action 20min Its bactericidal rate can reach more than 90%, and the sterilization efficiency of ozone is low when the TP content of ascites is low.

【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R187

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李富歡,劉紅生,黃六英,趙越,陳鐘鳴,高小燕,宋秋英;腹水濃縮回輸腹腔治療肝硬化難治性腹水的效果評(píng)價(jià)[J];實(shí)用肝臟病雜志;2005年03期

2 王芳,易濱,劉育京,李漢忠,胡松;蛋白胨對(duì)臭氧水殺菌作用影響的試驗(yàn)研究[J];中國公共衛(wèi)生;2002年04期

3 談智,吉鐘山,徐燕;臭氧對(duì)水中微生物的殺滅效果及其影響因素[J];中國消毒學(xué)雜志;1999年03期

4 鄭臨,楊益大,孔海深,魏澤慶,吳煒,馬亦林;肝硬化患者自發(fā)性腹膜炎細(xì)菌學(xué)及耐藥性的變遷[J];中華傳染病雜志;2001年03期

5 中華醫(yī)學(xué)會(huì)傳染病與,寄生蟲病學(xué)分會(huì),肝病學(xué)分會(huì);病毒性肝炎防治方案[J];中華肝臟病雜志;2000年06期

6 葉英,余鑫之,徐元宏;嚴(yán)重肝病腹腔感染的致病菌變遷和耐藥趨勢研究[J];中華肝臟病雜志;2001年04期

7 翁心華;自發(fā)性細(xì)菌性腹膜炎的一些共識(shí)與進(jìn)展[J];中華肝臟病雜志;2003年07期

8 秦波,郭樹華;自發(fā)性細(xì)菌性腹膜炎[J];中華肝臟病雜志;2003年07期

9 劉麗敏,池艷春,朱丹,楊曉梅,孫麗紅;體外濃縮自體腹水回輸治療肝硬化頑固性腹水的臨床觀察[J];中華肝臟病雜志;2004年05期

10 楊晉輝,余曙光,尤麗英,常江,李俊,徐嫦玉;自體腹水濃縮后腹腔回輸治療頑固性腹水[J];中華內(nèi)科雜志;1996年07期

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