【摘要】：目的：齲病是世界范圍內(nèi)廣泛存在的口腔常見疾病,我國患齲率高達(dá)90%以上。隨著口腔保健意識的增強,齲齒風(fēng)險評估和早期檢測成為口腔臨床醫(yī)學(xué)領(lǐng)域面臨的重要問題。本論文從自體熒光光譜技術(shù)角度展開研究,為無創(chuàng)、準(zhǔn)確、可定量的光學(xué)診斷技術(shù)應(yīng)用于齲病精確化防控奠定實驗基礎(chǔ)。 方法及結(jié)果： ①應(yīng)用熒光分光光度計對6種齲病相關(guān)微生物逐一進(jìn)行光譜表征參數(shù)分析,發(fā)現(xiàn)6種齲病相關(guān)微生物在隨著激發(fā)波長的遞增,發(fā)射波出現(xiàn)了紅移的現(xiàn)象；6種齲病相關(guān)微生物在350nm激發(fā)波長下,均存在436+4nm的特征熒光峰位值,提示此特征熒光來自于非卟啉物質(zhì)。加血培養(yǎng)促進(jìn)了齲病相關(guān)微生物的自體熒光代謝環(huán)境,微生物濃度與熒光強度之間存在線性正相關(guān)。 ②通過模擬口腔環(huán)境,形成不同致齲強度的人工菌斑,測試獲得菌斑熒光光譜表征參數(shù)。結(jié)果顯示培養(yǎng)第8天,1%蔗糖濃度組的菌斑溶液熒光強度高于其他蔗糖濃度組(P0.05)。部分實驗組熒光強度第8天高于第4天(P0.05)；采用逐步回歸分析方法設(shè)立了牙釉質(zhì)片微硬度下降百分比、細(xì)菌粘附度與熒光參數(shù)的函數(shù)關(guān)系。 ③采用熒光分光光度計獲得不同齲損程度離體牙在405nm波長激發(fā)下的熒光光譜分布特征,結(jié)果顯示熒光光譜面積比率值P與齲齒檢測儀DD值之間具有較好的線性關(guān)系(R=-0.9085)。分析了不同激光功率和不同檢測部位對熒光光譜表征參數(shù)的影響。 ④在健康及不同齲損程度離體牙的熒光光譜特征參數(shù)基礎(chǔ)上,分析了齲損等級與熒光面積比率值的關(guān)系(P0.01),齲損等級與主波長的關(guān)系(P0.01),熒光面積比率值α與熒光圖像分量比率p的關(guān)系(P0.01),根據(jù)ROC分析方法獲得了熒光圖像分量比率β的齲損分級標(biāo)準(zhǔn)。 結(jié)論：本文探索了齲病相關(guān)微生物、人工菌斑、健康及不同齲損程度牙體組織的自體熒光光譜特征；通過熒光強度對口腔微生物進(jìn)行定量化分析是可行的；研究了人工牙菌斑的熒光光譜表征性參數(shù)與其致齲性的關(guān)系；設(shè)立了基于齲損組織自體熒光光譜參數(shù)的熒光光譜面積比率值、熒光圖像分量比率值,為基于自體熒光技術(shù)的齲活躍性評價和齲損分級提供了理論依據(jù),為后期的齲齒檢測儀研發(fā)提供必要的實驗基礎(chǔ)。
[Abstract]:Objective: caries is a common oral disease in the world. The incidence of caries in China is as high as 90%. With the increasing awareness of oral health care, dental caries risk assessment and early detection have become an important problem in the field of oral clinical medicine. In this paper, the study was carried out from the point of view of autofluorescence spectroscopy, which laid an experimental foundation for the application of noninvasive, accurate and quantitative optical diagnosis technology in the accurate prevention and control of caries. Methods and results: (1) the spectral characterization parameters of six kinds of caries related microorganisms were analyzed by fluorescence spectrophotometer one by one. It was found that the emission waves of 6 kinds of caries related microorganisms shifted red with the increase of excitation wavelength. The characteristic fluorescence peaks of 436 4nm were found in all six caries-related microorganisms at 350nm excitation wavelength, suggesting that the characteristic fluorescence came from non-porphyrin substances. Blood culture promoted the autofluorescence metabolic environment of caries-related microorganisms, and there was a linear positive correlation between microbial concentration and fluorescence intensity. (2) artificial plaque with different caries intensity was formed by simulating oral environment, and the fluorescence spectrum characterization parameters of plaque were obtained. The results showed that on the 8th day of culture, the fluorescence intensity of plaque solution in 1% sucrose concentration group was higher than that in other sucrose concentration groups (P 0.05). The fluorescence intensity of some experimental groups on the 8th day was higher than that on the 4th day (P 0.05). The percentage of microhardness decrease and the functional relationship between bacterial adhesion and fluorescence parameters were established by stepwise regression analysis. (3) the distribution characteristics of fluorescence spectra of isolated teeth with different degrees of caries excited by 405nm wavelength were obtained by fluorescence spectrophotometer. The results showed that there was a good linear relationship between fluorescence spectral area ratio P and DD value of dental caries detector (R 鈮，

本文編號：2479339