高糖對(duì)人牙周膜細(xì)胞骨向分化的影響
發(fā)布時(shí)間：2019-01-12 19:19
【摘要】：目的：觀察體外高糖培養(yǎng)環(huán)境對(duì)人牙周膜細(xì)胞(periodontal ligament cells, PDLCs)增殖及骨向分化的影響。 方法：原代培養(yǎng)人牙周膜細(xì)胞,取4-6代細(xì)胞用于實(shí)驗(yàn)。運(yùn)用MTT法檢測(cè)正常組和高糖組牙周膜細(xì)胞在不同培養(yǎng)環(huán)境中的增殖活性。細(xì)胞在低糖和高糖DMEM配制的正常組礦化液和高糖組礦化液培養(yǎng)基(50mg/ml維生素C,10mMβ-磷酸甘油鈉和100nM地塞米松)中培養(yǎng)后,于第1,7,14,21和28天運(yùn)用茜素紅染色法觀察礦化結(jié)節(jié)形成,以及觀察不同培養(yǎng)環(huán)境下PDLCs堿性磷酸酶(Alkaline Phosphatase, ALP)活性和Ⅰ型膠原蛋白的表達(dá)。 結(jié)果：高糖能抑制牙周膜細(xì)胞的增殖；高糖礦化組和正常礦化組之間,礦化結(jié)節(jié)形成有明顯差異；高糖礦化組和正常礦化組ALP活性和Ⅰ型膠原蛋白表達(dá)有差異。 結(jié)論：高糖對(duì)牙周膜細(xì)胞增殖和骨向分化有抑制作用,進(jìn)一步解釋了糖尿病牙周炎患者病情嚴(yán)重且預(yù)后延遲的機(jī)制。
[Abstract]:Aim: to observe the effect of high glucose culture on (periodontal ligament cells, PDLCs) proliferation and bone differentiation of human periodontal ligament cells in vitro. Methods: human periodontal ligament cells were cultured in primary culture. The proliferative activity of periodontal ligament cells in normal and high glucose groups was detected by MTT method. The cells were cultured in 50mg/ml Vitamin C, 10mM 尾 -Glycerol Phosphate and 100nM Dexamethasone medium (50mg/ml Vitamin C, 10mM 尾 -Glycerol Phosphate and 100nM Dexamethasone) prepared with low glucose and high glucose DMEM. On day 21 and 28, alizarin red staining was used to observe the formation of mineralized nodules, the activity of PDLCs alkaline phosphatase (Alkaline Phosphatase, ALP) and the expression of type I collagen under different culture conditions. Results: high glucose could inhibit the proliferation of periodontal ligament cells, there were significant differences in the formation of mineralized nodules between high glucose mineralization group and normal mineralization group, and there were differences in ALP activity and type I collagen expression between high glucose mineralization group and normal mineralization group. Conclusion: high glucose can inhibit the proliferation and bone differentiation of periodontal ligament cells, which further explains the mechanism of severe disease and delayed prognosis in patients with diabetic periodontitis.
【學(xué)位授予單位】：武漢大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R781.4
[Abstract]:Aim: to observe the effect of high glucose culture on (periodontal ligament cells, PDLCs) proliferation and bone differentiation of human periodontal ligament cells in vitro. Methods: human periodontal ligament cells were cultured in primary culture. The proliferative activity of periodontal ligament cells in normal and high glucose groups was detected by MTT method. The cells were cultured in 50mg/ml Vitamin C, 10mM 尾 -Glycerol Phosphate and 100nM Dexamethasone medium (50mg/ml Vitamin C, 10mM 尾 -Glycerol Phosphate and 100nM Dexamethasone) prepared with low glucose and high glucose DMEM. On day 21 and 28, alizarin red staining was used to observe the formation of mineralized nodules, the activity of PDLCs alkaline phosphatase (Alkaline Phosphatase, ALP) and the expression of type I collagen under different culture conditions. Results: high glucose could inhibit the proliferation of periodontal ligament cells, there were significant differences in the formation of mineralized nodules between high glucose mineralization group and normal mineralization group, and there were differences in ALP activity and type I collagen expression between high glucose mineralization group and normal mineralization group. Conclusion: high glucose can inhibit the proliferation and bone differentiation of periodontal ligament cells, which further explains the mechanism of severe disease and delayed prognosis in patients with diabetic periodontitis.
【學(xué)位授予單位】：武漢大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R781.4
【參考文獻(xiàn)】
相關(guān)期刊論文 前5條
1 唐q 琪;鐘泉;李大蘭;姚麗艷;李艷芬;閆福華;;不同培養(yǎng)基對(duì)人牙周膜細(xì)胞生物學(xué)行為的影響[J];口腔生物醫(yī)學(xué);2011年03期
2 韓R，
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