【摘要】：目的正畸復(fù)發(fā)牙移動(dòng)的骨改建過(guò)程同時(shí)受成骨細(xì)胞和破骨細(xì)胞的持續(xù)作用。Odanacatib是一個(gè)高度選擇性的組織蛋白酶K抑制劑,阻斷破骨細(xì)胞的骨吸收效應(yīng),促進(jìn)成骨,但其在正畸后復(fù)發(fā)領(lǐng)域的應(yīng)用尚屬空白。本實(shí)驗(yàn)從odanacatib調(diào)節(jié)破骨細(xì)胞功能,抑制移動(dòng)牙復(fù)發(fā)的角度出發(fā),通過(guò)建立大鼠正畸牙移動(dòng)復(fù)發(fā)模型,在復(fù)發(fā)牙周圍局部注射odanacatib,進(jìn)一步了解正畸牙移動(dòng)復(fù)發(fā)過(guò)程骨改建機(jī)制,分析外源性odanacatib對(duì)正畸牙移動(dòng)復(fù)發(fā)距離、牙槽骨密度以及復(fù)發(fā)過(guò)程中TRAP、Cat K、IGF-Ⅰ表達(dá)的影響,探討odanacatib是否具有促進(jìn)牙齒穩(wěn)定的作用,以期為正畸臨床保持階段提供有效的輔助方法。方法選用30只SPF級(jí)大鼠,建立種植支抗牙齒移動(dòng)模型,60g力值近中移動(dòng)右上頜第一磨牙,加力3周去除加力裝置讓其復(fù)發(fā)。隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組,每組15只。實(shí)驗(yàn)組于右上第一磨牙周圍粘骨膜局部注射60μl、1.25μM odanacatib,每3天注射一次。對(duì)照組注射等量生理鹽水。復(fù)發(fā)0天和2周時(shí)制取大鼠上頜牙列模型,測(cè)量右上頜第一、二磨牙間距離,計(jì)算復(fù)發(fā)率(復(fù)發(fā)率=復(fù)發(fā)距離/牙齒移動(dòng)距離*100%)。復(fù)發(fā)2周后處死大鼠,立即截取包含右上頜3顆磨牙及其周圍牙槽骨的上頜骨,進(jìn)行Micro-CT掃描,測(cè)量右上頜第一磨牙根分叉處牙槽骨BMD和BVF值,分析牙槽骨骨小梁空間結(jié)構(gòu)。掃描后制作組織切片,HE染色觀察第一磨牙牙周組織形態(tài)學(xué)變化。TRAP特殊染色觀察牙周破骨細(xì)胞數(shù)量的變化,免疫組織化學(xué)染色觀察牙周組織Cat K和IGF-Ⅰ的表達(dá),利用圖像分析軟件對(duì)其含量進(jìn)行半定量分析。采用SPSS21.0統(tǒng)計(jì)軟件進(jìn)行分析。結(jié)果拆除矯治器后,2組大鼠第一磨牙均向遠(yuǎn)中移動(dòng)造成復(fù)發(fā),實(shí)驗(yàn)組復(fù)發(fā)率小于對(duì)照組,且實(shí)驗(yàn)組牙槽骨的BMD和BVF值高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義。HE染色鏡下觀察可見(jiàn)實(shí)驗(yàn)組牙周膜纖維排列規(guī)則,近遠(yuǎn)中側(cè)牙周膜間隙均勻,可見(jiàn)新骨形成。對(duì)照組牙周膜纖維排列比較規(guī)則,近遠(yuǎn)中側(cè)牙周膜間隙比較均勻,可見(jiàn)新的類骨質(zhì)填充。實(shí)驗(yàn)組TRAP陽(yáng)性染色細(xì)胞的IOD值小于對(duì)照組,但無(wú)統(tǒng)計(jì)學(xué)差異;實(shí)驗(yàn)組Cat K的IOD值小于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義;實(shí)驗(yàn)組IGF-Ⅰ的表達(dá)高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義。結(jié)論(1)局部注射odanacatib能有效地抑制破骨細(xì)胞Cat K的表達(dá),但不減少破骨細(xì)胞的數(shù)量,對(duì)破骨細(xì)胞的存活沒(méi)影響。(2)局部注射odanacatib減小大鼠正畸復(fù)發(fā)率,增加局部牙槽骨的BMD和BVF值。提示odanacatib在正畸牙復(fù)發(fā)過(guò)程中可以有效減少骨吸收,增加骨形成,抑制復(fù)發(fā)。(3)局部注射odanacatib增加牙周組織IGF-Ⅰ的分泌,提示odanacatib在正畸牙復(fù)發(fā)過(guò)程中保護(hù)膠原的降解,促進(jìn)牙周組織改建,增強(qiáng)移動(dòng)牙的穩(wěn)定性。
[Abstract]:Objective the process of bone remodeling in orthodontic recurrent tooth movement is sustained by both osteoblasts and osteoclasts. Odanacatib is a highly selective cathepsin K inhibitor that blocks osteoclast resorption and promotes osteogenesis. But its application in the field of orthodontic recurrence is still blank. From the point of view of odanacatib regulating osteoclast function and inhibiting the recurrence of moving teeth, we established a rat model of orthodontic tooth movement and recurrence, and injected odanacatib, around the recurrent teeth to further understand the mechanism of bone remodeling in the process of orthodontic tooth movement and recurrence. To analyze the effects of exogenous odanacatib on the distance of orthodontic tooth movement and recurrence, alveolar bone mineral density and the expression of TRAP,Cat KF- 鈪，

本文編號(hào)：2384913