【摘要】：背景:口腔黏膜的敏感性和分泌黏液的功能等增加了口腔軟組織修復(fù)難度,患者修復(fù)后其形態(tài)和功能難以達(dá)到預(yù)期的修復(fù)效果。納米纖維素蛋白主要由甘氨酸、丙氨酸以及絲氨酸等組成,該材料具有良好的組織相容性。但是,目前臨床上對于納米纖維素蛋白和脫細(xì)胞基質(zhì)在口腔黏膜修復(fù)中效果效果比較研究較少,且臨床上對于兩種材料修復(fù)效果尚存在較大爭議。目的:觀察納米纖維素蛋白和脫細(xì)胞基質(zhì)在口腔黏膜修復(fù)中的應(yīng)用,比較兩種不同修復(fù)材料的效果。方法:建立缺口黏膜缺損模型大鼠,建模后隨機(jī)分為4組,對照組大鼠采用凡士林油修復(fù),納米纖維素蛋白組大鼠采用納米纖維素蛋白修復(fù),牛脫細(xì)胞基質(zhì)組采用牛皮膚組織脫細(xì)胞基質(zhì)修復(fù),人脫細(xì)胞基質(zhì)組則利用人皮膚組織脫細(xì)胞基質(zhì)修復(fù)。各組大鼠進(jìn)行2個月觀察,比較不同材料下大鼠口腔黏膜中的修復(fù)效果。結(jié)果與結(jié)論:(1)口腔黏膜缺損直徑:采用游標(biāo)卡尺對4組口腔黏膜修復(fù)進(jìn)行測量,各組大鼠術(shù)后1 d口腔黏膜缺損直徑差異無顯著性意義(P0.05),納米纖維素蛋白組大鼠術(shù)后3,5,7 d口腔黏膜缺損直徑顯著低于其他3組(P0.05),牛脫細(xì)胞基質(zhì)組和人脫細(xì)胞基質(zhì)組在術(shù)后5,7 d口腔黏膜缺損直徑顯著低于對照組(P0.05);(2)口腔黏膜組織形態(tài):光學(xué)顯微鏡下觀察顯示,納米纖維素蛋白組、牛脫細(xì)胞基質(zhì)組和人脫細(xì)胞基質(zhì)組在術(shù)后1,3,5,7周口腔黏膜缺損部分新生組織中毛細(xì)血管內(nèi)皮數(shù)差異無顯著性意義(P0.05);納米纖維素蛋白組、牛脫細(xì)胞基質(zhì)組和人脫細(xì)胞基質(zhì)組與對照組相比差異有顯著性意義(P0.05);納米纖維素蛋白組術(shù)后21 d新生上皮較厚,排列比較緊密;牛脫細(xì)胞基質(zhì)組術(shù)后21 d缺損部位修復(fù)良好,新生上皮存在,炎性細(xì)胞減少;人脫細(xì)胞基質(zhì)組存在明顯炎性細(xì)胞,存在新生上皮組織,厚度一般;對照組修復(fù)21 d后炎性細(xì)胞較多,上皮組織較薄。(3)結(jié)果說明,納米纖維素蛋白材料和脫細(xì)胞基質(zhì)能促進(jìn)口腔黏膜上皮增生,加快創(chuàng)面愈合。
[Abstract]:Background: the sensitivity of oral mucosa and mucus secreting function increase the difficulty of repairing oral soft tissue, and the shape and function of patients are difficult to achieve the desired repair effect. Nanocellulose protein is mainly composed of glycine, alanine and serine, which has good histocompatibility. However, there are few clinical studies on the effects of nano-cellulose protein and acellular matrix in oral mucosal repair. Aim: to observe the application of nano-cellulose protein and acellular matrix in oral mucosal repair and compare the effects of two kinds of repair materials. Methods: the rats with notched mucosal defect were randomly divided into 4 groups. The control rats were repaired with vaseline oil and the rats with nano-cellulose protein were repaired with nano-cellulose protein. Bovine acellular matrix group was repaired by acellular matrix of bovine skin and human acellular matrix group was repaired by human skin acellular matrix. Rats in each group were observed for 2 months to compare the repair effect of oral mucosa in rats with different materials. Results and conclusion: (1) the diameter of oral mucosal defect was measured by Vernier caliper. There was no significant difference in the diameter of oral mucosal defect 1 day after operation in each group (P0.05). The diameter of oral mucosal defect in nano-cellulose protein group was significantly lower than that in the other three groups (P0.05). The diameter of oral mucosal defect in bovine acellular matrix group and human acellular matrix group was significantly lower than that in control group on the 7th day after operation (P0.05). (2) Morphology of oral mucosa: observed under optical microscope, nano-cellulose protein group, bovine acellular matrix group and human acellular matrix group were observed at 1: 3 after operation. There was no significant difference in the number of capillary endothelium in some new tissues of oral mucosal defect at 5 ~ 7 weeks (P0.05). Nanocellulose protein group, bovine acellular matrix group and human acellular matrix group had significant differences compared with the control group (P0.05). The defect site of bovine acellular matrix group was repaired well 21 days after operation, the neonate epithelium existed and the inflammatory cells decreased, while in the human acellular matrix group, there were obvious inflammatory cells, new epithelium and normal thickness. In the control group, there were more inflammatory cells and thinner epithelium after 21 days of repair. (3) the results showed that nano-cellulose protein material and acellular matrix could promote the proliferation of oral mucosal epithelium and accelerate wound healing.
【作者單位】： 西南醫(yī)科大學(xué)口頜面修復(fù)重建和再生實驗室;西南醫(yī)科大學(xué)附屬口腔醫(yī)院牙周黏膜科;西南醫(yī)科大學(xué)附屬口腔醫(yī)院修復(fù)科;
【基金】：四川醫(yī)科大學(xué)口腔醫(yī)學(xué)院省級大學(xué)生創(chuàng)新訓(xùn)練計劃項目(201510632020);四川醫(yī)科大學(xué)口腔醫(yī)學(xué)院校級大學(xué)生創(chuàng)新訓(xùn)練計劃項目(2015131);四川醫(yī)科大學(xué)附屬口腔醫(yī)院院級課題;四川醫(yī)科大學(xué)2015年國家級大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃項目(201510632020) 西南醫(yī)科大學(xué)附屬口腔醫(yī)院院級課題(201507)~~
【分類號】：R783
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本文編號：2379428