【摘要】：目的:比較大鼠不同直徑牙胚細胞增殖和骨向分化能力的差異。方法:酶消化法分離、培養(yǎng)SD大鼠牙胚細胞,經(jīng)13μm標準細胞篩分選后獲得大、小2種直徑的細胞;并分別采用MTT法檢測2種細胞的增殖能力;堿性磷酸酶(ALP)試劑盒、茜素紅染色法及qRT-PCR檢測兩者的骨向分化能力。結果:小細胞的增殖能力較低(P0.05);培養(yǎng)3、7 d后,小細胞組的ALP活性均明顯高于大細胞組(P0.05);培養(yǎng)14 d后,小細胞組鈣化結節(jié)數(shù)量明顯高于大細胞組;qRT-PCR檢測結果顯示,小細胞組中Ocn、Osx、Runx2各礦化相關基因的表達水平均明顯高于大細胞組(P0.05)。結論:牙胚細胞中直徑小于13μm的細胞比直徑大于13μm的細胞增殖活性低,分化能力強。
[Abstract]:Aim: to compare the proliferation and bone differentiation ability of rat tooth germ cells with different diameters. Methods: SD rat tooth germ cells were isolated and cultured by enzyme digestion method. After screening with 13 渭 m standard cells, two different diameters were obtained, and the proliferative ability of two kinds of cells were detected by MTT method. Alkaline phosphatase (ALP) kit, alizarin red staining and qRT-PCR were used to detect the ability of bone differentiation. Results: the proliferative ability of small cells was lower (P0.05), the activity of ALP in small cell group was significantly higher than that in large cell group after 3 days of culture (P0.05), the number of calcified nodules in small cell group was significantly higher than that in large cell group after 14 days culture (P0.05). QRT-PCR analysis showed that the expression level of Ocn,Osx,Runx2 mineralization related genes in small cell group was significantly higher than that in large cell group (P0.05). Conclusion: the proliferative activity and differentiation ability of tooth germ cells with diameter less than 13 渭 m are lower than those with diameter more than 13 渭 m.
【作者單位】： 南京醫(yī)科大學口腔醫(yī)學研究所;南京醫(yī)科大學附屬口腔醫(yī)院牙體牙髓病科;
【基金】：國家自然科學基金資助項目(81371144)
【分類號】：R781

【參考文獻】

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