【摘要】：目的研究高遷移率族蛋白N2(HMGN2)對(duì)人舌癌裸鼠移植瘤生長的抑瘤作用。方法裸鼠腋下注射接種舌鱗癌細(xì)胞Tca8113以建立裸鼠移植瘤模型,接種7 d成瘤后,設(shè)立陰性對(duì)照組、HMGN2蛋白組和陽性對(duì)照組,在瘤體周邊分別注射含washingbufferⅡ、HMGN2蛋白和順鉑的細(xì)胞培養(yǎng)基。觀察各組裸鼠腫瘤的生長情況,經(jīng)過4次給藥后處死裸鼠,取出瘤塊,稱其質(zhì)量計(jì)算抑瘤率,并行蘇木精-伊紅(HE)染色觀察形態(tài)學(xué)變化。結(jié)果成功建立舌癌裸鼠移植瘤模型。HMGN2蛋白組和陽性對(duì)照組腫瘤體積明顯小于陰性對(duì)照組。裸鼠體重在整個(gè)實(shí)驗(yàn)過程中并沒有明顯的變化。陰性對(duì)照組、HMGN2蛋白組、陽性對(duì)照組的腫瘤質(zhì)量平均值分別為(0.38±0.19)、(0.21±0.15)、(0.23±0.16)g,3組間的差異無統(tǒng)計(jì)學(xué)意義。HMGN2蛋白組和陽性對(duì)照組的抑瘤率分別為45.71%和39.44%。腫瘤經(jīng)肉眼觀察可見陰性對(duì)照組瘤塊較大,HE染色可見HMGN2蛋白組和陽性對(duì)照組細(xì)胞壞死明顯。結(jié)論 HMGN2蛋白可以明顯抑制人舌癌裸鼠移植瘤的生長。
[Abstract]:Objective to study the inhibitory effect of high mobility group protein N2 (HMGN2) on the growth of human tongue cancer xenografts in nude mice. Methods the transplanted tumor model of nude mice was established by subaxillary injection of tongue squamous carcinoma cell line Tca8113. After 7 days of inoculation, negative control group, HMGN2 protein group and positive control group were established. Washingbuffer 鈪，

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