潤滑液體注入的多孔釉質(zhì)表面的生物清污性能的研究
發(fā)布時間：2018-11-03 13:52
【摘要】：目的本文主要探討構(gòu)建潤滑液體注入的多孔釉質(zhì)表面后,該表面對于唾液黏蛋白的吸附以及細(xì)菌生物膜形成的影響。方法制備若干尺寸為4×4×2mm3牛牙釉質(zhì)片,備用。用37%的磷酸酸蝕1min后沖洗,然后隨機(jī)分為四組。以1H,1H,2H,2H-全氟癸基三氯硅烷和全氟三戊胺依次處理釉質(zhì)片為實(shí)驗(yàn)組,以去離子水處理的釉質(zhì)片為對照組1,僅以全氟三戊胺處理的釉質(zhì)片為對照組2以及以1H,1H,2H,2H-全氟癸基三氯硅烷處理的釉質(zhì)片為對照組3。用傅里葉紅外光譜儀以及原子力顯微鏡檢測各組樣品表面,并檢測樣品表面水接觸角,其中每組測量六個樣品。采用酶標(biāo)儀檢測各組樣品表面唾液黏蛋白黏附后,用阿新藍(lán)溶液染色后的洗脫液在595nm下的光密度值,以檢測唾液黏蛋白吸附情況。用場發(fā)射掃描電子顯微鏡觀測各組樣品在4小時,24小時以及48小時后表面細(xì)菌生物膜生長情況。并用菌落形成單位計數(shù)法計算各組樣品表面三個時間組的細(xì)菌菌落數(shù),每組有六個樣品。在動物實(shí)驗(yàn)中,以在新西蘭大白兔的一側(cè)前牙上構(gòu)建的潤滑液體多孔釉質(zhì)表面為實(shí)驗(yàn)組,對側(cè)同名牙僅以35%磷酸凝膠酸蝕為對照組。飼以高糖飲食,并于48小時后用菌斑指示劑染色,觀察兩組前牙的牙菌斑染色情況。結(jié)果傅里葉紅外光譜和原子力顯微鏡顯示實(shí)驗(yàn)組1H,1H,2H,2H-全氟癸基三氯硅烷和全氟三戊胺均吸附于多孔釉質(zhì)表面,潤滑液體多孔釉質(zhì)表面構(gòu)建成功。其中原子力顯微鏡下可見,實(shí)驗(yàn)組樣品表面粗糙度明顯小于三個對照組樣本。對照組1、對照組2、對照組3以及實(shí)驗(yàn)組的水接觸角結(jié)果差異有統(tǒng)計學(xué)意義(P0.05)。對照組1、對照組2、對照組3以及實(shí)驗(yàn)組的黏蛋白吸附洗脫液的光密度值結(jié)果差異有統(tǒng)計學(xué)意義(P0.05)。實(shí)驗(yàn)組樣品表面在4小時后幾乎無細(xì)菌生長而在3個對照組中均只有少量菌落形成。24小時后,實(shí)驗(yàn)組樣品表面有少量稀疏的菌落形成, 而對照組樣品表面有交織的細(xì)菌菌落密集分布,但仍可見樣品表面。在48小時后,實(shí)驗(yàn)組樣品表面仍然只有少量菌落分布,與24小時組相比,視野中菌落增加較少。而三個對照組樣品表面均被均勻且厚的菌落密集覆蓋,釉質(zhì)表面完全不可見。由菌落形成單位計數(shù)結(jié)果可見,在三個時間段實(shí)驗(yàn)組樣品表面菌落計數(shù)均少于三個對照組,差異有統(tǒng)計學(xué)意義(P0.05)。由新西蘭大白兔體內(nèi)實(shí)驗(yàn)可見,48小時后,實(shí)驗(yàn)組牙齒牙菌斑染色明顯少于對照組。結(jié)論本研究構(gòu)建的潤滑液體多孔釉質(zhì)表面可以有效抑制唾液黏蛋白的吸附以及細(xì)菌生物膜的形成。
[Abstract]:Objective to investigate the effect of the porous enamel surface implanted with lubricating fluid on the adsorption of salivary mucin and the formation of bacterial biofilm. Methods A number of 4 脳 4 脳 2mm3 bovine enamel tablets were prepared and set aside. 1min was washed with 37% phosphoric acid and then randomly divided into four groups. The enamel tablets treated with 1H ~ (-1) H ~ (2) fluorodecyl trichlorosilane and perfluorotripentylamine in turn were used as the experimental group, the enamel tablets treated with deionized water as the control group (1), only the enamel tablets treated with perfluorotripentylamine as the control group, and the enamel tablets treated The enamel treated with 2 H 2 H-perfluorodecyl trichlorosilane was the control group 3. FT-IR and AFM were used to detect the surface of each group of samples, and the water contact angle of each group of samples was measured. Six samples were measured in each group. The adhesion of salivary mucin on the surface of each group was detected by enzyme labeling instrument, and the optical density of the eluate stained with azin blue solution was measured under 595nm to detect the adsorption of salivary mucin. The growth of bacterial biofilm was observed by field emission scanning electron microscope (SEM) after 4 hours, 24 hours and 48 hours. The number of bacterial colonies in three time groups of each group was calculated by colony forming unit count method, and there were six samples in each group. In animal experiment, the porous enamel surface of lubricated liquid was constructed on one front tooth of New Zealand white rabbit as experimental group, and the control group was only 35% phosphoric acid etching on the opposite side of homonym teeth. The dental plaque staining of the anterior teeth of the two groups was observed after 48 hours of high glucose diet and stain with plaque indicator. Results the results of Fourier transform infrared spectroscopy and atomic force microscope showed that the experimental group (1H ~ (-1) H ~ (2) H _ (2) F _ (3) H _ (3) Chlorosilane and PFC _ (3) were adsorbed on the surface of porous enamel. The surface roughness of the experimental group was obviously less than that of the three control group samples under atomic force microscope (AFM). The water contact angle of control group 1, control group 2, control group 3 and experimental group were significantly different (P0.05). In control group 1, control group 2, control group 3 and experimental group of mucin adsorption eluate optical density value difference was statistically significant (P0.05). There was almost no bacterial growth on the surface of the samples in the experimental group after 4 hours, but only a small number of colonies were formed in the three control groups. 24 hours later, a small number of sparse colonies formed on the surface of the samples in the experimental group. In the control group, there were interlaced bacterial colonies on the surface of the sample, but the surface of the sample was still visible. After 48 hours, there were only a few colonies on the surface of the samples in the experimental group, compared with the 24 hour group, the colony increased less in the visual field. The surface of the three control groups was covered with uniform and thick colony, and the enamel surface was completely invisible. From the results of colony formation unit count, the surface colony count of the experimental group was less than that of the three control groups in three time periods, the difference was statistically significant (P0.05). After 48 hours, dental plaque staining in the experimental group was significantly less than that in the control group. Conclusion the lubricated porous enamel surface can effectively inhibit the adsorption of salivary mucin and the formation of bacterial biofilm.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R783
本文編號：2307984
[Abstract]:Objective to investigate the effect of the porous enamel surface implanted with lubricating fluid on the adsorption of salivary mucin and the formation of bacterial biofilm. Methods A number of 4 脳 4 脳 2mm3 bovine enamel tablets were prepared and set aside. 1min was washed with 37% phosphoric acid and then randomly divided into four groups. The enamel tablets treated with 1H ~ (-1) H ~ (2) fluorodecyl trichlorosilane and perfluorotripentylamine in turn were used as the experimental group, the enamel tablets treated with deionized water as the control group (1), only the enamel tablets treated with perfluorotripentylamine as the control group, and the enamel tablets treated The enamel treated with 2 H 2 H-perfluorodecyl trichlorosilane was the control group 3. FT-IR and AFM were used to detect the surface of each group of samples, and the water contact angle of each group of samples was measured. Six samples were measured in each group. The adhesion of salivary mucin on the surface of each group was detected by enzyme labeling instrument, and the optical density of the eluate stained with azin blue solution was measured under 595nm to detect the adsorption of salivary mucin. The growth of bacterial biofilm was observed by field emission scanning electron microscope (SEM) after 4 hours, 24 hours and 48 hours. The number of bacterial colonies in three time groups of each group was calculated by colony forming unit count method, and there were six samples in each group. In animal experiment, the porous enamel surface of lubricated liquid was constructed on one front tooth of New Zealand white rabbit as experimental group, and the control group was only 35% phosphoric acid etching on the opposite side of homonym teeth. The dental plaque staining of the anterior teeth of the two groups was observed after 48 hours of high glucose diet and stain with plaque indicator. Results the results of Fourier transform infrared spectroscopy and atomic force microscope showed that the experimental group (1H ~ (-1) H ~ (2) H _ (2) F _ (3) H _ (3) Chlorosilane and PFC _ (3) were adsorbed on the surface of porous enamel. The surface roughness of the experimental group was obviously less than that of the three control group samples under atomic force microscope (AFM). The water contact angle of control group 1, control group 2, control group 3 and experimental group were significantly different (P0.05). In control group 1, control group 2, control group 3 and experimental group of mucin adsorption eluate optical density value difference was statistically significant (P0.05). There was almost no bacterial growth on the surface of the samples in the experimental group after 4 hours, but only a small number of colonies were formed in the three control groups. 24 hours later, a small number of sparse colonies formed on the surface of the samples in the experimental group. In the control group, there were interlaced bacterial colonies on the surface of the sample, but the surface of the sample was still visible. After 48 hours, there were only a few colonies on the surface of the samples in the experimental group, compared with the 24 hour group, the colony increased less in the visual field. The surface of the three control groups was covered with uniform and thick colony, and the enamel surface was completely invisible. From the results of colony formation unit count, the surface colony count of the experimental group was less than that of the three control groups in three time periods, the difference was statistically significant (P0.05). After 48 hours, dental plaque staining in the experimental group was significantly less than that in the control group. Conclusion the lubricated porous enamel surface can effectively inhibit the adsorption of salivary mucin and the formation of bacterial biofilm.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R783
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