【摘要】：目的通過檢測不同濃度的生物活性玻璃浸提液對小鼠L929細胞的增殖活性、細胞周期和骨向分化的作用,為生物活性玻璃在牙周組織再生領域的應用提供基礎實驗依據。方法本實驗研究分為兩部分:1生物活性玻璃對L929細胞增殖的影響。制備生物活性玻璃浸提液,以10%小牛血清的RPMI1640完全培養(yǎng)液稀成不同濃度的生物活性玻璃(bioactive glass,BG)浸提液培養(yǎng)L929細胞24h、48h和72h后,采用四唑鹽(MTT)法和流式細胞術(FCM)法檢測不同濃度的生物活性玻璃浸提液對L929細胞增殖活性和細胞周期的影響。2生物活性玻璃對L929細胞骨向分化的影響。采用堿性磷酸酶(ALP)活性測定的方法,分別在24h、48h和72h檢測不同濃度的生物活性玻璃浸提液對體外培養(yǎng)L929細胞骨向分化的影響。結果MTT和ALP法測定結果均顯示培養(yǎng)L929細胞24h、48h和72h后,隨時間延長,0.0625g/L~1.25g/L組的OD值均高于對照組,差異有統(tǒng)計學意義(P0.05),以0.0625g/L濃度組的OD值最高(P0.05);2.5g/L組的OD值較對照組稍低,差異無統(tǒng)計學意義(P0.05);5g/L組與對照組相比OD值顯著降低,差異有統(tǒng)計學意義(P0.05)。FCM實驗結果與上述實驗結果相符,BG浸提液濃度為0.0625g/L時細胞增殖指數(shù)(PI)最高,濃度為5g/L時PI值最低(P0.05)。結論1生物活性玻璃在一定濃度范圍內可促進L929成纖維細胞的增殖及骨向化。2生物活性玻璃在低濃度時促進L929細胞增殖和骨向分化,高濃度時抑制L929細胞的增殖和骨向分化。
[Abstract]:Objective to investigate the effects of different concentrations of bioactive glass extract on the proliferation, cell cycle and bone differentiation of mouse L929 cells, and to provide a basic experimental basis for the application of bioactive glass in the field of periodontal tissue regeneration. Methods the experiment was divided into two parts: 1 the effect of bioactive glass on the proliferation of L 929 cells. The bioactive glass extract was prepared and the L929 cells were incubated with 10% bovine serum RPMI1640 complete culture medium with different concentrations of bioactive glass (bioactive glass,BG) for 48 h and 72 h. The effects of different concentrations of bioactive glass extract on the proliferation activity and cell cycle of L929 cells were detected by (MTT) and flow cytometry. 2 the effects of bioactive glass on bone differentiation of L929 cells were studied. The effects of different concentrations of bioactive glass extract on bone differentiation of L929 cells in vitro were determined by alkaline phosphatase (ALP) assay at 24 h and 72 h, respectively. Results the results of MTT and ALP assay showed that the OD value of 0.0625g/L~1.25g/L group was higher than that of control group (P0.05), the OD value of 0.0625g/L group was the highest (P0.05), the OD value of 2.5g/L group was slightly lower than that of control group. There was no significant difference (P0.05); compared with the control group, the OD value of 5g/L group was significantly lower than that of the control group (P0.05). FCM experimental results were consistent with the above experimental results, the highest (PI) cell proliferation index (PI) when the concentration of BG extract solution was 0.0625g/L, PI value was the lowest when 5g/L concentration was P05). Conclusion (1) Bioactive glass can promote the proliferation and osteotropism of L929 fibroblasts in a certain concentration range. (2) Bioactive glass can promote the proliferation and bone differentiation of L929 cells at low concentration, and inhibit the proliferation and bone differentiation of L929 cells at high concentration.
【學位授予單位】：華北理工大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R781.42

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