發(fā)布時間：2018-10-12 13:39

【摘要】：目的探索低氧誘導(dǎo)因子-1α(HIF-1α)基因誘導(dǎo)牙髓干細(xì)胞(DPSCs)在體外的成血管作用。方法對HIF-1α進(jìn)行基因突變,構(gòu)建突變型、野生型以及對照組的慢病毒載體;體外培養(yǎng)DPSCs;分別用3種慢病毒轉(zhuǎn)染DPSCs后,檢測細(xì)胞轉(zhuǎn)染效率、目的基因HIF-1α在mRNA及蛋白水平的表達(dá),MTT法檢測慢病毒載體對細(xì)胞增殖的影響;目的基因轉(zhuǎn)染成功后,q PCR、Western blot法檢測HIF-1α調(diào)控DPSCs成血管因子的表達(dá)。結(jié)果 MTT結(jié)果表明慢病毒載體對DPSCs的增殖幾乎無影響。q PCR和Western blot法檢測目的基因HIF-1α成功表達(dá),HIF-1α能夠顯著上調(diào)DPSCs的成血管因子的表達(dá)(P0.05)。突變組和野生組的成血管作用明顯強(qiáng)于對照組(P0.05),而突變組又優(yōu)于野生組(P0.05)。結(jié)論 HIF-1α基因可以促進(jìn)DPSCs血管向分化作用。
[Abstract]:Objective to investigate the vascularization of dental pulp stem cell (DPSCs) induced by hypoxia inducible factor-1 偽 (HIF-1 偽) gene in vitro. Methods Lentivirus vectors of mutant type, wild type and control group were constructed by gene mutation of HIF-1 偽, DPSCs; was transfected into DPSCs by three kinds of lentiviruses in vitro, and the transfection efficiency was measured. Objective to detect the expression of HIF-1 偽 gene at the level of mRNA and protein, to detect the effect of lentivirus vector on cell proliferation by MTT method, and to detect the expression of DPSCs angiogenic factor regulated by HIF-1 偽 after gene transfection. Results the results of MTT showed that lentivirus vector had little effect on the proliferation of DPSCs and the expression of target gene HIF-1 偽 was detected successfully by. Q PCR and Western blot. HIF-1 偽 could significantly up-regulate the expression of angiogenic factors of DPSCs (P0.05). The vascularization effect of mutant group and wild group was significantly stronger than that of control group (P0.05), but the mutation group was superior to wild group (P0.05). Conclusion HIF-1 偽 gene can promote the differentiation of DPSCs vessels.
【作者單位】： 安徽醫(yī)科大學(xué)口腔醫(yī)學(xué)院安徽醫(yī)科大學(xué)附屬口腔醫(yī)院安徽省口腔疾病研究中心實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(編號:31501103)
【分類號】：R781
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本文編號：2266324