有機硅季銨鹽溶液浸泡后純鈦抗菌性能的研究
發(fā)布時間：2018-08-07 19:07
【摘要】：目的:本實驗旨在研究經(jīng)不同濃度的有機硅季銨鹽溶液浸泡后純鈦磨片的抗菌性能以及抗菌持續(xù)時間。方法:1抗菌性實驗完成打磨拋光、滅菌處理后,將120個鈦片隨機分為A、B、C、D、E、F六個大組,每組20片。6個大組又隨機分為1、2、3、4四個亞組,每組5片。A組:采用1%濃度有機硅季銨鹽溶液浸泡;B組:采用5%濃度有機硅季銨鹽溶液浸泡;C組:采用10%濃度有機硅季銨鹽溶液浸泡;D組:采用30%濃度有機硅季銨鹽溶液浸泡;E組:采用60%濃度有機硅季銨鹽溶液浸泡;F組:采用滅菌蒸餾水浸泡。四個亞組分別處理時間為:1組30 min,2組60 min,3組120 min,4組240min。處理完畢后取出放入滅菌蒸餾水中漂洗10min,取出,自然晾干后置入無菌24孔細(xì)胞培養(yǎng)板中。肺炎鏈球菌標(biāo)準(zhǔn)菌株ATCC49619復(fù)蘇后,采用1640液體培養(yǎng)基進行稀釋,濁度儀調(diào)整菌液濃度為0.5M。將不同分組的試件分別放入1ml菌懸液中浸泡,并放入37℃恒溫箱中培養(yǎng)24h。取出試件,滅菌蒸餾水清洗試件上的浮菌,再將試件放入3ml1640液體培養(yǎng)基中,于振蕩器上振蕩1min,將液體培養(yǎng)基轉(zhuǎn)移到比濁專用管中,混合均勻后使用濁度儀進行菌液濃度計數(shù)。采用無菌1640液體培養(yǎng)基作為空白對照。上述實驗結(jié)果進行統(tǒng)計分析,發(fā)現(xiàn)1%有機硅季銨鹽浸泡120min組為菌液濃度最低、抗菌性能最佳組,采取此種浸泡方式進行下面實驗。2抗菌性持續(xù)時間實驗選取純鈦試件30個,分為G、H、I、J、K五組,每組6個樣本:G組經(jīng)脫脂滅菌后采用1%有機硅季銨鹽浸泡120min,將試件循環(huán)浸入5℃與55℃的恒溫水浴中分別浸泡30s,循環(huán)0次后取出;H組經(jīng)脫脂滅菌后采用1%有機硅季銨鹽浸泡120min,將試件循環(huán)浸入5℃與55℃的恒溫水浴中分別浸泡30s,循環(huán)2500次后取出;i組經(jīng)脫脂滅菌后采用1%有機硅季銨鹽浸泡120min,將試件循環(huán)浸入5℃與55℃的恒溫水浴中分別浸泡30s,循環(huán)5000次后取出;j組組經(jīng)脫脂滅菌后采用1%有機硅季銨鹽浸泡120min,將試件循環(huán)浸入5℃與55℃的恒溫水浴中分別浸泡30s,循環(huán)10000次后取出;k組僅采用脫脂滅菌處理,用蒸餾水進行浸泡處理。將處理好的各組試件分別放入1ml的0.5m菌懸液中,恒溫箱37℃培養(yǎng)24h,取出試件,滅菌蒸餾水清洗試件上的浮菌,再將試件放入3ml1640液體培養(yǎng)基中,于振蕩器上振蕩1min,將液體培養(yǎng)基轉(zhuǎn)移到比濁專用管中,混合均勻后使用濁度儀進行菌液濃度計數(shù)。仍采用無菌1640液體培養(yǎng)基作為空白對照。3數(shù)據(jù)處理采用spss13.0軟件對本次研究所得數(shù)據(jù)進行統(tǒng)計學(xué)處理,選用kruskal-wallish非參數(shù)檢驗對抗菌性能進行分析,lsd檢驗進行兩兩比較,p0.05代表有統(tǒng)計學(xué)意義。選用dunnett-t檢驗對抗菌性時間實驗數(shù)據(jù)進行統(tǒng)計學(xué)處理,p0.05代表有統(tǒng)計學(xué)意義。結(jié)果:1抗菌性測定結(jié)果不同濃度間的主效應(yīng)存在差異,p0.05;但是對于不同處理時間之間的主效應(yīng)無統(tǒng)計學(xué)意義,p0.05;兩者之間存在交互作用,p0.05。對處理濃度因素進行分析比較結(jié)果顯示:a、b、c、d、e、f這六組數(shù)據(jù)中,a組與b組,c組與d組、e組之間無差別;其余任意兩組間數(shù)據(jù)比較均有差異p0.05。經(jīng)過分析發(fā)現(xiàn)abcde五組與f組間數(shù)據(jù)有差異p0.05,說明a組1%濃度的處理能夠讓純鈦產(chǎn)生抗菌性。2抗菌性持續(xù)時間測定結(jié)果g組、h組、i組菌液濃度均低于k組,差異有統(tǒng)計學(xué)差異,p0.05;j組與k組之間進行比較無統(tǒng)計學(xué)差異,p0.05。結(jié)論:1有機硅季銨鹽溶液浸泡純鈦后,能夠讓純鈦具備抗菌性能,其最適宜處理濃度為1%,最適宜處理時間為120min。2經(jīng)1%有機硅季銨鹽溶液浸泡純鈦120min后,其抗菌性能能夠保持6個月左右。
[Abstract]:Objective: the purpose of this experiment was to study the antibacterial properties and duration of the pure titanium grinding tablets soaked with different concentrations of organosilicon quaternary ammonium salt solution. Methods: after 1 antibacterial experiments were finished, the 120 titanium slices were randomly divided into six groups, A, B, C, D, E, F, and each group of 20 pieces of.6 were randomly divided into four subgroups of 1,2,3,4, and were randomly divided into four subgroups of 1,2,3,4. Each group of 5 groups of.A groups were soaked in 1% concentration of silicone quaternary ammonium salt solution; group B: 5% concentration of silicone quaternary ammonium salt solution; group C: 10% concentration organosilicon quaternary ammonium salt solution; D group: 30% concentration organosilicon quaternary ammonium salt solution; E group: 60% concentration of silicon quaternary ammonium salt solution; F group: steeped distilled water soaked in sterilized water. The treatment time of the four subgroups was 30 min, 2 group 60 min, 3 group 120 min. 4 groups of 240min. were removed and rinsed into the sterile distilled water to rinse 10min, then put out, and then put into the sterile 24 hole cell culture plate after natural drying. The standard strain ATCC49619 of Streptococcus pneumoniae was resuscitation with 1640 liquid medium, and the turbidimeter adjusted the bacterial liquid. The concentration is 0.5M., the samples of different groups are soaked in 1ml bacteria suspension, and the specimens are incubated in the constant temperature box of 37 C, and the specimens are extracted by 24h., then the floating bacteria are cleaned by the sterilization distilled water. Then the specimens are put into the 3ml1640 liquid medium, and the oscillator oscillates 1min in the oscillator, and the Ye Tipei's foster group is transferred to the turbid special tube, and the mixture is used evenly after the use. The bacterial liquid concentration was counted by the turbidimeter. The aseptic 1640 liquid medium was used as the blank control. The results of the experiment were statistically analyzed. The results showed that 1% organosilicon quaternary ammonium salt soaked in 120min group was the lowest, and the best antibacterial performance group. This immersion method was used to test the antibacterial duration of the following experimental.2 for the selection of pure titanium specimen 30 G, H, I, J, K five groups, each group of 6 samples: G group after degreasing sterilization with 1% organosilicon quaternary ammonium salt soak 120min, immerse the specimen in 5 centigrade and 55 C in the constant temperature water bath respectively, respectively, after 0 times to take out, the H group after degreasing sterilization with 1% organosilicon quaternary ammonium salt soak 120min, immerse the test parts at 5 and 55 C constant temperature water The 30s was soaked in the bath for 2500 times, and the I group was soaked with 1% organosilicon quaternary ammonium salt for 120min after degreasing and sterilizing. The samples were soaked in 5 C and 55 C in the constant temperature water bath respectively for 30s, and then taken out for 5000 times. The j group was soaked with 1% organosilicon quaternary ammonium salt after degreasing and sterilizing, and the specimens were immersed in 5 and 55 degrees centigrade. 30s was soaked in the warm water bath and removed for 10000 times. The K group was treated with only degreasing sterilization and distilled water to soak. The treated samples were put into the 0.5m suspension of 1ml respectively. The incubators were incubated at 37 degrees centigrade for 24h, and the specimens were removed, and the floating bacteria were cleaned by sterilized distilled water, and then the specimens were put into the liquid medium of 3ml1640. The liquid medium was transferred to 1min, the liquid medium was transferred to the turbidimetric special tube, and the concentration of the bacteria was counted using the turbidimeter. The sterile 1640 liquid medium was still used as the blank control.3 data processing, and the data of this study were processed by the SPSS13.0 software, and the non parameter test of kruskal-wallish was selected. Analysis of antibacterial properties, LSD test for 22 comparison, P0.05 representative of statistical significance. Select the dunnett-t test for the antibacterial time experimental data processing, P0.05 representative of statistical significance. Results: 1 the main effect of different concentrations of antimicrobial determination, the main effect is different, P0.05; but for different treatment time There is no statistical significance between the main effect of P0.05 and the interaction between the two. The results of the analysis and comparison of the concentration factors of p0.05. show that there are no differences between the a group and the B group in the six groups of data of a, B, C, D, e and F, and there is no difference between the C group and the D group and the other two groups. The difference P0.05 shows that the treatment of a group 1% concentration can make pure titanium produce antibacterial.2 antibacterial duration determination result G group, H group and I group are lower than K group, the difference has statistical difference, P0.05, J group and K group have no statistical difference, p0.05. conclusion: 1 silicone quaternary ammonium salt solution after pure titanium, can make pure titanium. The optimum treatment concentration is 1%, the most suitable treatment time is 120min.2 after 1% organosilicon quaternary ammonium salt solution of pure titanium 120min, its antibacterial properties can be maintained for about 6 months.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R783.1
本文編號：2171064
[Abstract]:Objective: the purpose of this experiment was to study the antibacterial properties and duration of the pure titanium grinding tablets soaked with different concentrations of organosilicon quaternary ammonium salt solution. Methods: after 1 antibacterial experiments were finished, the 120 titanium slices were randomly divided into six groups, A, B, C, D, E, F, and each group of 20 pieces of.6 were randomly divided into four subgroups of 1,2,3,4, and were randomly divided into four subgroups of 1,2,3,4. Each group of 5 groups of.A groups were soaked in 1% concentration of silicone quaternary ammonium salt solution; group B: 5% concentration of silicone quaternary ammonium salt solution; group C: 10% concentration organosilicon quaternary ammonium salt solution; D group: 30% concentration organosilicon quaternary ammonium salt solution; E group: 60% concentration of silicon quaternary ammonium salt solution; F group: steeped distilled water soaked in sterilized water. The treatment time of the four subgroups was 30 min, 2 group 60 min, 3 group 120 min. 4 groups of 240min. were removed and rinsed into the sterile distilled water to rinse 10min, then put out, and then put into the sterile 24 hole cell culture plate after natural drying. The standard strain ATCC49619 of Streptococcus pneumoniae was resuscitation with 1640 liquid medium, and the turbidimeter adjusted the bacterial liquid. The concentration is 0.5M., the samples of different groups are soaked in 1ml bacteria suspension, and the specimens are incubated in the constant temperature box of 37 C, and the specimens are extracted by 24h., then the floating bacteria are cleaned by the sterilization distilled water. Then the specimens are put into the 3ml1640 liquid medium, and the oscillator oscillates 1min in the oscillator, and the Ye Tipei's foster group is transferred to the turbid special tube, and the mixture is used evenly after the use. The bacterial liquid concentration was counted by the turbidimeter. The aseptic 1640 liquid medium was used as the blank control. The results of the experiment were statistically analyzed. The results showed that 1% organosilicon quaternary ammonium salt soaked in 120min group was the lowest, and the best antibacterial performance group. This immersion method was used to test the antibacterial duration of the following experimental.2 for the selection of pure titanium specimen 30 G, H, I, J, K five groups, each group of 6 samples: G group after degreasing sterilization with 1% organosilicon quaternary ammonium salt soak 120min, immerse the specimen in 5 centigrade and 55 C in the constant temperature water bath respectively, respectively, after 0 times to take out, the H group after degreasing sterilization with 1% organosilicon quaternary ammonium salt soak 120min, immerse the test parts at 5 and 55 C constant temperature water The 30s was soaked in the bath for 2500 times, and the I group was soaked with 1% organosilicon quaternary ammonium salt for 120min after degreasing and sterilizing. The samples were soaked in 5 C and 55 C in the constant temperature water bath respectively for 30s, and then taken out for 5000 times. The j group was soaked with 1% organosilicon quaternary ammonium salt after degreasing and sterilizing, and the specimens were immersed in 5 and 55 degrees centigrade. 30s was soaked in the warm water bath and removed for 10000 times. The K group was treated with only degreasing sterilization and distilled water to soak. The treated samples were put into the 0.5m suspension of 1ml respectively. The incubators were incubated at 37 degrees centigrade for 24h, and the specimens were removed, and the floating bacteria were cleaned by sterilized distilled water, and then the specimens were put into the liquid medium of 3ml1640. The liquid medium was transferred to 1min, the liquid medium was transferred to the turbidimetric special tube, and the concentration of the bacteria was counted using the turbidimeter. The sterile 1640 liquid medium was still used as the blank control.3 data processing, and the data of this study were processed by the SPSS13.0 software, and the non parameter test of kruskal-wallish was selected. Analysis of antibacterial properties, LSD test for 22 comparison, P0.05 representative of statistical significance. Select the dunnett-t test for the antibacterial time experimental data processing, P0.05 representative of statistical significance. Results: 1 the main effect of different concentrations of antimicrobial determination, the main effect is different, P0.05; but for different treatment time There is no statistical significance between the main effect of P0.05 and the interaction between the two. The results of the analysis and comparison of the concentration factors of p0.05. show that there are no differences between the a group and the B group in the six groups of data of a, B, C, D, e and F, and there is no difference between the C group and the D group and the other two groups. The difference P0.05 shows that the treatment of a group 1% concentration can make pure titanium produce antibacterial.2 antibacterial duration determination result G group, H group and I group are lower than K group, the difference has statistical difference, P0.05, J group and K group have no statistical difference, p0.05. conclusion: 1 silicone quaternary ammonium salt solution after pure titanium, can make pure titanium. The optimum treatment concentration is 1%, the most suitable treatment time is 120min.2 after 1% organosilicon quaternary ammonium salt solution of pure titanium 120min, its antibacterial properties can be maintained for about 6 months.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R783.1
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 徐韋鋒;張振林;;TC4/TA15異質(zhì)鈦合金激光焊焊縫的顯微組織和力學(xué)性能（英文）[J];Transactions of Nonferrous Metals Society of China;2016年12期
2 陳靖剛;牛亞輝;劉杉杉;郝勃坤;左云鳳;;高齡老年人重癥吸入性肺炎臨床分析[J];河北醫(yī)藥;2016年22期
3 陳長會;史偉;彭擁軍;熊維;袁林;高薇;程小平;姚開虎;;中國西部單中心二級醫(yī)院呼吸道感染住院患兒鼻咽部肺炎鏈球菌攜帶及其血清型和耐藥性的橫斷面調(diào)查[J];中國循證兒科雜志;2016年04期
4 熊瑋;曾玉蘭;楊榮時;;老年吸入性肺炎病原學(xué)及臨床分析[J];臨床內(nèi)科雜志;2016年08期
5 李曉嵐;;口腔護理改善口腔疾病的效果研究[J];臨床醫(yī)藥文獻(xiàn)電子雜志;2016年07期
6 方榮;施榮;歐陽洋;韓丹;王倩;;老年吸入性肺炎的診治策略[J];中國急救醫(yī)學(xué);2016年02期
7 郝丹;李傳花;王志峰;王會;郭美畫;藍(lán)菁;;968名老年人可摘局部義齒戴用及口腔衛(wèi)生情況調(diào)查[J];中華老年口腔醫(yī)學(xué)雜志;2016年01期
8 徐永霞;李娟;李保同;劉澤學(xué);段久芳;韓春蕊;查顯俊;;非季銨鹽型松香基表面活性劑的研究進展[J];材料導(dǎo)報;2016年01期
9 林苑云;馮盈;蔡朱妹;;夜間戴用活動義齒與老年肺炎發(fā)生風(fēng)險的相關(guān)性研究[J];哈爾濱醫(yī)藥;2015年04期
10 劉妮;鄭則廣;陳萍;侯鵬;王欣妮;李洪誼;陳榮昌;;鼻咽部分泌物誤吸的檢測及其與老年肺炎發(fā)病的關(guān)系[J];中華結(jié)核和呼吸雜志;2015年07期
相關(guān)碩士學(xué)位論文 前1條
1 周唯;DMAEMA-PMDPM鹽抗菌單體的合成、相關(guān)性能及其應(yīng)用于牙科粘接劑的初步研究[D];第四軍醫(yī)大學(xué);2012年
，本文編號：2171064
本文鏈接：http://sikaile.net/yixuelunwen/kouq/2171064.html
最近更新
教材專著
