發(fā)布時間：2018-06-27 13:22

  本文選題：細胞外基質金屬蛋白酶誘導因子 + 親環(huán)素A　； 參考：《安徽醫(yī)科大學》2016年碩士論文

【摘要】：目的檢測細胞外基質金屬蛋白酶誘導因子(Extracellular Matrix Metalloproteinase Inducer,CD147,EMMPRIN)和親環(huán)素A(Cyclophilin A,Cyp A)在人根尖肉芽腫和根尖囊腫中的表達,探討CD147和Cyp A在人慢性根尖周炎發(fā)生發(fā)展中的作用。方法從2014年12月至2015年5月,在安徽醫(yī)科大學附屬口腔醫(yī)院口腔頜面外科收集經拔牙或根尖手術切除,并經病理鑒定為根尖肉芽腫(35例)的和根尖囊腫(30例)的組織作為實驗組,同時收集行牙槽骨修整術鑿下的或埋伏阻生智齒拔除時8例健康牙槽骨作為正常對照組。根據(jù)CBCT圖像記錄平均根尖周透射影直徑大小作為根尖周病變大小。運用免疫組織化學法檢測所有樣本中CD147和Cyp A蛋白的表達,根據(jù)病變類型(根尖肉芽腫、根尖囊腫)和根尖周病變大小,分析CD147和Cyp A的蛋白表達水平。結果免疫組織化學的連續(xù)切片顯示:CD147和Cyp A主要表達于炎性和上皮區(qū)域,主要為淋巴細胞、上皮細胞、巨噬細胞、成纖維細胞等。CD147和Cyp A存在于所有根尖周病變組織中。正常對照組中少見CD147、Cyp A表達,陰性對照組中未見CD147、Cyp A陽性表達。CD147、Cyp A在根尖肉芽腫和根尖囊腫中的蛋白表達水平均顯著高于對照組(P0.05),另外,CD147、Cyp A在根尖囊腫中的表達明顯高于在根尖肉芽腫中的表達(P=0.004、P=0.000)。CD147和Cyp A的蛋白表達水平在根尖肉芽腫(r=0.787,P0.05)和根尖囊腫(r=0.755,P0.05)中呈正相關;CD147和Cyp A的表達水平均與慢性根尖周炎的病變大小呈正相關(R2線性=0.746,R2線性=0.934,P0.05)。結論1.本實驗通過免疫組織化學的方法,發(fā)現(xiàn)CD147在根尖肉芽腫和根尖囊腫中的蛋白表達水平均顯著高于對照組,證實CD147存在于人慢性根尖周炎病損組織中。2.CD147、Cyp A在根尖囊腫中的表達明顯高于在根尖肉芽腫中的表達,且CD147和Cyp A的蛋白表達水平在根尖囊腫和根尖肉芽腫中呈正相關,提示CD147和Cyp A可能參與根尖周病損的發(fā)生發(fā)展。3.CD147和Cyp A的表達水平均與慢性根尖周炎的病變大小呈正相關,說明CD147-Cyp A相互作用可能參與炎性反應和骨質吸收,且在人慢性根尖周病的發(fā)生發(fā)展過程中可能發(fā)揮了某種協(xié)同作用。
[Abstract]:Objective to investigate the expression of extracellular matrix metalloproteinase inducer CD147 EMMPRIN and cyclophilin A in human apical granuloma and apical cyst, and to explore the role of CD147 and Cyp A in the development of chronic apical periapical periodontitis. Methods from December 2014 to May 2015, dental and maxillofacial surgery was performed at the Oral and Maxillofacial Hospital of Anhui Medical University. The tissues of 35 cases of apical granuloma (35 cases) and 30 cases of apical cyst (30 cases) were identified by pathology as experimental group, and 8 cases of healthy alveolar bone were collected as normal control group. The mean periapical diameter was recorded by CBCT images as the periapical lesion size. The expression of CD147 and Cyp A in all samples was detected by immunohistochemical method. The expression levels of CD147 and Cyp A were analyzed according to the type of lesion (apical granuloma, cysts) and the size of periapical lesions. Results Immunohistochemical serial sections showed that cell CD147 and Cyp A were mainly expressed in inflammatory and epithelial regions, mainly lymphocytes, epithelial cells, macrophages, fibroblasts, and so on. CD147 and Cyp A were found in all periapical lesions. The expression of CD147 Cyp A was rare in normal control group. The positive expression of CD147 Cyp A in apical granuloma and apical cyst was significantly higher than that in control group (P0.05). In addition, the expression of CD147 Cyp A in apical cyst was significantly higher than that in apical granuloma. The expression levels of CD147 and Cyp A were positively correlated with the size of chronic periapical periodontitis (R2 linear 0.746R 2 linear 0.934 P 0.05) in apical granuloma (rn 0.787 P 0.05) and apical cysts (rn 0.755 P 0.05), and the expression levels of CD147 and Cyp A were positively correlated with the size of chronic periapical periodontitis (R 2 0. 746%, R 2 0. 746 0. 05 P 0.05), and the expression levels of CD147 and Cyp A were positively correlated with the size of chronic periapical periodontitis. Conclusion 1. The expression of CD147 protein in apical granuloma and apical cyst was significantly higher than that in control group by immunohistochemical method. The expression of CD147 in the lesions of chronic apical periodontitis was significantly higher than that in the apical granuloma, and the expression of CD147 and Cyp A was positively correlated with the apical cyst and apical granuloma. The results suggest that CD147 and Cyp A may be involved in the occurrence and development of periapical lesions. 3. The expression levels of CD147 and Cyp A are positively correlated with the size of chronic periapical disease, indicating that CD147-Cyp A interaction may be involved in inflammatory reaction and bone resorption. It may play a synergistic role in the occurrence and development of chronic periapical disease.
【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R781.341

【相似文獻】

相關期刊論文 前10條

1 馮利,馮潔;根尖肉芽腫細胞超微結構觀察[J];現(xiàn)代口腔醫(yī)學雜志;2002年03期

2 馮利,馮潔;根尖肉芽腫中免疫細胞超微結構的研究[J];蘭州大學學報;2002年05期

3 王勇,史濱琦,孫明華;綜合治療根尖肉芽腫52例體會[J];黑龍江醫(yī)學;2004年06期

4 白東升;李利;;基質金屬蛋白酶-1和8在根尖肉芽腫中的表達及意義[J];包頭醫(yī)學院學報;2011年02期

5 付改秀;根尖肉芽腫致拔牙后出血6例報告[J];實用口腔醫(yī)學雜志;1988年02期

6 林居紅;;根尖肉芽腫的銀質沉積物[J];國外醫(yī)學.口腔醫(yī)學分冊;1988年05期

7 蔣月桂;;根尖肉芽腫中的托通樣巨細胞[J];國外醫(yī)學.口腔醫(yī)學分冊;1990年05期

8 蔭俊,金巖,張郁,史俊南;根尖肉芽腫中腫瘤壞死因子的免疫組織化學研究[J];實用口腔醫(yī)學雜志;1992年03期

9 江鳳英;根尖肉芽腫致拔牙后出血6例報告[J];牙病防治雜志;1994年01期

10 曹穎光，，王燕秋，鄧惠妹;根尖肉芽腫內厭氧菌與臨床表現(xiàn)的關系[J];臨床口腔醫(yī)學雜志;1995年01期

相關會議論文 前3條

1 胡曉春;陳建芳;;基質金屬蛋白酶-8mRNA在根尖肉芽腫中的表達及意義[A];2007年第七次全國牙體牙髓病學學術會議論文集[C];2007年

2 胡曉春;李漢玲;隋曉光;;MMP-2、9在根尖肉芽腫和根尖囊腫組織中的表達及意義[A];全國第三次牙體牙髓病學臨床技術研討會論文匯編[C];2009年

3 李月;王冠英;;正常、炎癥牙髓及根尖肉芽腫中IL-1、TNF的免疫組化研究[A];全國第四次牙體牙髓病學術會議論文匯編[C];1995年

相關碩士學位論文 前5條

1 胡菊花;白介素-21和破骨細胞核因子κB受體活化因子配體在人根尖肉芽腫和根尖囊腫中的表達及臨床意義[D];安徽醫(yī)科大學;2015年

2 胡淑麗;CD147和CypA在人慢性根尖周炎中的表達及臨床意義[D];安徽醫(yī)科大學;2016年

3 李漢玲;基質金屬蛋白酶-2、9在根尖肉芽腫和根尖囊腫的表達及意義[D];吉林大學;2008年

4 陳建芳;基質金屬蛋白酶-8 mRNA在根尖肉芽腫和根尖囊腫的表達及意義[D];吉林大學;2007年

5 郎小英;Th17細胞在人慢性根尖周炎中的定位研究[D];安徽醫(yī)科大學;2014年

本文編號：2074052