本文選題：細胞毒性 + 粘結材料�。� 參考：《昆明醫(yī)科大學》2017年碩士論文

【摘要】：[目的]通過CCK-8法和流式細胞術的檢測,觀察了解玻璃離子水門汀、聚羧酸鋅水門汀、3M自粘結樹脂粘固劑三種粘結材料對小鼠成纖維細胞L929的細胞增殖率和細胞凋亡率的影響及差異,為臨床應用提供實驗依據(jù)。[方法]用CCK-8法檢測三種粘結材料浸提3d、7d、14d三個時間段的細胞增殖率(relative growth rate,RGR)和流式細胞術檢測三種粘結材料浸提3d、7d兩個時間段的細胞凋亡率,觀察三種粘結材料對小鼠成纖維細胞L929的影響和差異。[結果]1.玻璃離子水門汀的 RGR 分別為 86.84%(3d)、82.85%(7d)、84.27%(14d),細胞毒性均為1級,表現(xiàn)為輕微細胞毒性。不同浸提時間組之間無明顯差異(P0.05)。2.聚羧酸鋅水門汀的 RGR 分別為 79.26%(3d)、77.93%(7d)、72.47%(14d),3d和7d細胞毒性為1級,14d細胞毒性為2級,表現(xiàn)為輕微細胞毒性。不同浸提時間組之間無明顯差異(P0.05)。3.3M自粘結樹脂粘固劑的RGR分別為114.34%(3d)、116.57%(7d)、114.84%(14d),細胞毒性為0級,未表現(xiàn)出細胞毒性。不同浸提時間組之間無明顯差異(P0.05)。[結論]1.聚羧酸鋅水門汀3d,7d兩個時間組的細胞毒性為1級,表現(xiàn)輕微細胞毒性;14d時間組的細胞毒性為2級,表現(xiàn)為輕度細胞毒性;2.玻璃離子水門汀組三個時間組的細胞毒性均為1級,表現(xiàn)出輕微的細胞毒性;3.3M自粘結樹脂粘固劑的細胞毒性則為0級,無細胞毒性;4.三種粘結材料均滿足口腔材料臨床應用的生物相容性的要求。
[Abstract]:[objective] to observe and understand glass ionomer cement by CCK-8 method and flow cytometry. Effects and differences of three kinds of adhesive materials on cell proliferation and apoptosis of mouse fibroblasts L929, which provides experimental basis for clinical application. [methods] the cell proliferation rate and relative growth rateRGRs of three kinds of adhesive materials were measured by CCK-8 and flow cytometry respectively. The apoptotic rates of three kinds of adhesive materials were detected at 3 days and 7 days after extraction. The effects and differences of three kinds of adhesive materials on mouse fibroblasts L 929 were observed. [result] 1. The RGR of vitreous ionomer cement was 86.84 d ~ 82.85 and 84.27 ~ 14 d ~ (-1) respectively. The cytotoxicity of glass ionomer was grade 1, showing slight cytotoxicity. There was no significant difference between different extraction time groups. The RGR of zinc polycarboxylate cement was 79.26 days and 77.93 days, respectively. The cytotoxicity of zinc polycarboxylate was 72.47 days and 14days respectively. The cytotoxicity was grade 1, grade 14, and grade 2, showing slight cytotoxicity. There was no significant difference between different extraction time groups. The RGR of self-adhesive resin binder was 114.343d ~ 116.577d and 114.84d ~ 14d, respectively. The cytotoxicity was 0 grade, no cytotoxicity was found. There was no significant difference between different extraction time groups (P 0.05). [conclusion] 1. The cytotoxicity of zinc polycarboxylate cement for 3 days and 14 days was grade 1 and grade 2, respectively. The cytotoxicity of glass-ionomer cement group was grade 1, and the cytotoxicity of glass-ionomer cement group was grade 0 and no cytotoxicity. All three kinds of adhesive materials meet the requirements of biocompatibility for clinical application of oral materials.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R783.1

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