本文選題：慢性間歇低氧 + 牙周炎�。� 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:本實(shí)驗(yàn)的目的是模擬阻塞型睡眠呼吸暫停低通氣綜合征(obstructive sleep apnea-hypopnea syndrome,OSAHS)病人的間歇性低氧條件,來探討大鼠齦溝液中牙齦卟啉單胞菌的變化。方法:將32只雄性SD大鼠隨機(jī)分成四組:常氧對照組(A組)、常氧牙周炎組(B組)、間歇性低氧組(C組)、間歇性低氧合并牙周炎組(D組)。采用正畸結(jié)扎絲結(jié)扎雙側(cè)上頜第二磨牙頸部和高糖飲食方法建立牙周炎模型,常氧組和低氧組分別在常氧條件和模擬阻塞性睡眠呼吸暫停低通氣綜合征條件下飼養(yǎng),8周后,對各組大鼠進(jìn)行牙周檢查,記錄牙周各項(xiàng)臨床指標(biāo),同時采集每只大鼠上頜右側(cè)第二磨牙齦溝液標(biāo)本,然后用空氣栓塞法處死各組大鼠,取出上頜骨,觀察牙槽骨吸收情況。采用實(shí)時熒光定量PCR方法檢測齦溝液中牙齦卟啉單胞菌的變化。結(jié)果:1.建模第8周,B組和D組大鼠實(shí)驗(yàn)牙食物堆積,牙齦紅腫,探診出血,有深的牙周袋形成,而A組和C組大鼠實(shí)驗(yàn)牙牙周組織變化不大,牙齦基本正常。說明牙周炎模型建立成功。2.四組大鼠牙周臨床檢查:D組大鼠牙周臨床檢查指標(biāo)高于其他各組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);B組大鼠臨床檢查指標(biāo)高于A組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。C組大鼠臨床檢查指標(biāo)高于A組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。3.D組牙槽骨較其他各組吸收明顯。4.所有的大鼠均能檢出牙齦卟啉單胞菌。D組牙齦卟啉單胞菌的量明顯高于其他各組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);B組牙齦卟啉單胞菌的量高于A組、C組兩組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。而A組和C組相比,牙齦卟啉單胞菌的量差異無統(tǒng)計(jì)學(xué)意義。結(jié)論:1.本實(shí)驗(yàn)成功地建立慢性間歇性低氧條件下大鼠牙周炎模型。2.牙齦卟啉單胞菌為口腔齦溝液中的常駐菌。慢性間歇性低氧條件下齦溝液中牙齦卟啉單胞菌數(shù)量增加,加重大鼠牙周炎的病變程度。
[Abstract]:Objective: to investigate the changes of porphyromonas gingivalis in rat gingival crevicular fluid (GCF) by simulating intermittent hypoxia in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods: Thirty-two male Sprague-Dawley rats were randomly divided into four groups: group A (normoxic control group), group B (normoxic periodontitis group), group C (intermittent hypoxia group) and group D (intermittent hypoxia combined with periodontitis group). The periodontitis model was established by orthodontic ligation of bilateral maxillary second molar neck and high glucose diet. The rats in normoxic and hypoxic groups were fed with obstructive sleep apnea hypopnea syndrome (OSAS) for 8 weeks. Periodontal examination was performed on each group of rats, and the clinical indexes of periodontal were recorded. At the same time, the gingival crevicular fluid samples of the second grinding on the right side of the maxilla were collected, then the rats were killed by air embolization, the maxillary bone was removed and the alveolar bone resorption was observed. The changes of porphyromonas gingivalis in gingival crevicular fluid were detected by real-time fluorescent quantitative PCR. The result is 1: 1. In the 8th week of modeling, the experimental teeth of group B and group D had accumulated food, gingival redness, bleeding and deep periodontal pocket formation, but the periodontal tissue of group A and group C had little change, and the gingiva was basically normal. The results showed that the model of periodontitis was established successfully. The periodontal clinical examination in group D was higher than that in other groups, and the difference was statistically significant. The clinical examination index of group B was higher than that of group A, and the difference of clinical examination index of group C was higher than that of group A. The difference was statistically significant (P 0.05). 3.The alveolar bone in group D was significantly higher than that in other groups. All rats were able to detect the amount of porphyromonas gingivalis in group D, which was significantly higher than that in other groups, and the difference was statistically significant in group B than in group A and group C, and the difference was statistically significant (P 0.05). There was no significant difference in the quantity of porphyromonas gingivalis between group A and group C. Conclusion 1. In this experiment, the rat periodontitis model was successfully established under chronic intermittent hypoxia. Porphyromonas gingivalis is a resident bacteria in oral gingival crevicular fluid. Under chronic intermittent hypoxia, the quantity of porphyromonas gingivalis in gingival crevicular fluid increased, which aggravated the severity of periodontitis in rats.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R781.4

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