本文選題：磷酸酶及張力蛋白同源基因蛋白 + 口腔鱗狀細(xì)胞癌��； 參考：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2017年01期

【摘要】：目的研究第10號(hào)染色體缺失的磷酸酶及張力蛋白同源基因蛋白(phosphatase and tensin homolog deleted on chromosome ten,PTEN)在口腔鱗狀細(xì)胞癌(oral squamous cell carcinoma,OSCC)中的表達(dá)及其臨床意義。方法收集30例OSCC及30例距癌組織邊界2 cm的癌旁組織,Western blot測(cè)定上述組織PTEN蛋白含量,qRT-PCR測(cè)定mRNA的表達(dá)水平,免疫組化(immunohistochemistry,IHC)定性測(cè)定PTEN蛋白在上述組織細(xì)胞中的組織學(xué)定位與表達(dá)情況,并根據(jù)PTEN表達(dá)結(jié)果分析其與OSCC臨床病理關(guān)系。結(jié)果 1癌旁組織均為PTEN蛋白陽(yáng)性表達(dá),PTEN蛋白陽(yáng)性表達(dá)主要為細(xì)胞漿內(nèi)棕黃色或棕褐色染色。OSCC組織多為陰性表達(dá)。2OSCC癌組織中PTEN(蛋白和mRNA)的表達(dá)明顯低于2 cm外的癌旁組織(P0.05)。3不同年齡、不同性別、不同發(fā)生部位OSCC的PTEN(蛋白和mRNA)表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。4PTEN在高分化鱗癌中的表達(dá)明顯較中低分化鱗癌高(P0.05)。5無(wú)淋巴轉(zhuǎn)移的較有淋巴轉(zhuǎn)移的表達(dá)高(P0.05)。6臨床Ⅰ+Ⅱ期表達(dá)明顯高于Ⅲ+Ⅳ期(P0.05)。結(jié)論 PTEN在OSCC癌組織中存在不同程度的異常表達(dá),可能是口腔鱗癌發(fā)生、發(fā)展、侵襲轉(zhuǎn)移過(guò)程中的一個(gè)重要因素。
[Abstract]:Objective to study the expression and clinical significance of phosphatase and tensin homolog deleted on chromosome tenon (PTEN), a homologous protein of phosphatase and tension protein homologous gene, deleted on chromosome 10 in oral squamous cell carcinoma (OSCC). Methods 30 cases of OSCC and 30 cases of paracancerous tissues 2 cm from the margin of cancer were collected to detect the PTEN protein content and the expression of mRNA by qRT-PCR. Immunohistochemical staining (IHC) was used to determine the histological localization and expression of PTEN protein in these tissues. The clinicopathological relationship between IHC and OSCC was analyzed according to the results of PTEN expression. Results (1) the positive expression of PTEN protein was mainly brown or brown staining in the cytoplasm. The expression of PTEN (protein and mRNAs) was significantly lower than that outside 2 cm. P0. 05. 3 in adjacent tissues of cancer. Different sexes, There was no significant difference in the expression of PTEN (protein and mRNAs) between different sites of OSCC. The expression of P0.05N in highly differentiated squamous cell carcinoma was significantly higher than that in poorly differentiated squamous cell carcinoma (MSCC) without lymphatic metastasis. The expression of P0.05mRNAs was significantly higher than that in patients with lymphatic metastasis. It was significantly higher than that in stage 鈪，

本文編號(hào)：1899284