本文選題：殼聚糖 + 纖維蛋白原�。� 參考：《中國組織工程研究》2017年10期

【摘要】：背景:隨著組織工程技術(shù)的飛速發(fā)展,單一的生物支架材料很難滿足組織工程的需要,通過合適的方法將兩種或多種類型的材料組合起來,可以得到性能更加優(yōu)良的復(fù)合支架材料。目的:檢測(cè)牙髓干細(xì)胞在殼聚糖-纖維蛋白原復(fù)合支架材料上的黏附與增殖情況。方法:(1)采用改良酶消化法分離提取C57乳鼠牙髓干細(xì)胞,傳代培養(yǎng)至第3代,體外成脂成骨誘導(dǎo)分化對(duì)細(xì)胞進(jìn)行鑒定;(2)制備殼聚糖-纖維蛋白原復(fù)合支架材料,測(cè)定其孔徑及孔隙率;(3)將殼聚糖-纖維蛋白原復(fù)合支架材料與第3代小鼠牙髓干細(xì)胞共培養(yǎng),以細(xì)胞直接接種于培養(yǎng)板底部作為對(duì)照組,MTT法檢測(cè)細(xì)胞增殖情況;(4)掃描電鏡觀察殼聚糖-纖維蛋白原復(fù)合支架材料的形態(tài),細(xì)胞黏附、增殖及基質(zhì)分泌情況。結(jié)果與結(jié)論:(1)成功分離培養(yǎng)C57小鼠牙髓干細(xì)胞,經(jīng)成脂成骨誘導(dǎo)分化后結(jié)果呈陽性;(2)殼聚糖-纖維蛋白原復(fù)合支架材料孔徑為(105.32±22.10)μm,孔隙率為(87.714±1.276)%;(3)牙髓干細(xì)胞在殼聚糖-纖維蛋白原復(fù)合支架材料上的增殖曲線與對(duì)照組基本一致,均為"S"型,培養(yǎng)第4-8天實(shí)驗(yàn)組增殖速度顯著快于對(duì)照組,差異有顯著性意義(P0.05);(4)共培養(yǎng)第2天,細(xì)胞與殼聚糖-纖維蛋白原復(fù)合支架材料黏附緊密,生長狀態(tài)良好,第4天,細(xì)胞明顯開始增殖,體積增大,有豐富的細(xì)胞外基質(zhì),第6天,鋪滿于整個(gè)材料表面及空隙;(5)殼聚糖-纖維蛋白原復(fù)合材料是一種適宜牙髓干細(xì)胞黏附與增殖的支架材料。
[Abstract]:Background: with the rapid development of tissue engineering technology, it is difficult for a single biological scaffold material to meet the needs of tissue engineering. More excellent composite scaffold materials can be obtained. Aim: to investigate the adhesion and proliferation of dental pulp stem cells on chitosan-fibrinogen composite scaffolds. Methods the dental pulp stem cells of C57 rat were isolated by modified enzyme digestion method and cultured to the third generation. The cells were identified by adipogenic osteogenesis induction differentiation in vitro. The chitosan fibrinogen composite scaffold was prepared. The chitosan / fibrinogen composite scaffold was co-cultured with the third generation mouse dental pulp stem cells by measuring its pore size and porosity. Cell proliferation was detected by MTT method. The morphology, cell adhesion, proliferation and matrix secretion of chitosan fibrinogen composite scaffold were observed by SEM. Results and conclusion: C57 mouse dental pulp stem cells were isolated and cultured successfully. The results of adipogenic osteogenesis induced differentiation were positive. The pore size of chitosan / fibrinogen composite scaffold was 105.32 鹵22.10 渭 m, the porosity was 87.714 鹵1.276%. The proliferation curve of dental pulp stem cells on chitosan fibrinogen composite scaffold was basically the same as that of control group. The proliferation rate of the experimental group was significantly faster than that of the control group on the 4-8 days after culture, and the difference was significant (P 0.05). On the second day of co-culture, the cells adhered closely to the chitosan / fibrinogen composite scaffold material, and the growth state was good, and on the 4th day, the growth rate of the experimental group was better than that of the control group. On the 6th day, chitosan / fibrinogen composite was a suitable scaffold for the adhesion and proliferation of dental pulp stem cells.
【作者單位】： 新疆醫(yī)科大學(xué)第一附屬醫(yī)院兒童口腔科-口腔預(yù)防科;四川大學(xué)華西口腔醫(yī)院兒童口腔科;
【基金】：國家自然科學(xué)基金(81560178)~~
【分類號(hào)】：R783.1

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