本文選題：尼古丁　切入點(diǎn)：美卡拉明　出處：《國際口腔醫(yī)學(xué)雜志》2017年04期

【摘要】：目的探討尼古丁和美卡拉明對主要牙周致病微生物生長及生物膜形成的影響。方法采用二倍稀釋法,研究尼古丁對牙齦卟啉單胞菌和伴放線放線桿菌的最低抑菌濃度(MIC)和最低殺菌濃度(MBC)。通過酶標(biāo)儀測定吸光度值,研究在1/16 MIC、1/8 MIC、1/4 MIC、1/2 MIC、MIC等不同濃度的尼古丁對這2種細(xì)菌生物膜形成的影響。結(jié)果尼古丁對2種細(xì)菌的菌懸液的MIC均為8 mg·mL~(-1),MBC為16 mg·mL~(-1)。尼古丁濃度逐漸增加時,生物膜吸光度值逐漸增大;繼續(xù)增加濃度,吸光度值減小;在8 mg·mL~(-1)時,吸光度值減小到最低值,最低生物膜抑制濃度為8 mg·mL~(-1)。添加尼古丁受體拮抗劑美卡拉明組較對照組吸光度值高。結(jié)論小劑量的尼古丁能增加牙齦卟啉單胞菌和伴放線放線桿菌的生物膜形成,濃度繼續(xù)增加則抑制生物膜形成,加入美卡拉明后降低尼古丁濃度,更能明顯觀察到尼古丁對兩種細(xì)菌生物膜的促進(jìn)作用。
[Abstract]:Objective to investigate the effects of nicotine and megalamine on the growth and biofilm formation of major periodontal pathogenic microorganisms. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of nicotine against porphyromonas gingivalis and Actinobacillus actinomycetes were studied. The effect of different concentrations of nicotine on biofilm formation was studied at 1 / 16 MIC1 / 8 MIC1 / 4 MIC1 / 2 MICMIC. The results showed that the MIC of nicotine to bacterial suspensions of both bacteria was 8 mg / mL / 1 MBC = 16 mg mL / L -1 路min ~ (-1). The nicotine concentration increased gradually. The absorbance value of biofilm increases gradually; the absorbance value decreases with increasing concentration; at 8 mg / ml), the absorbance value decreases to the lowest value, The minimum inhibitory concentration of biofilm was 8 mg / mL ~ (-1). The concentration of nicotine receptor antagonist Mica Lemine was higher than that of the control group. Conclusion small doses of nicotine can increase the biofilm formation of Porphyromonas gingivalis and actinobacillus actinomycetes. When the concentration increased, the formation of biofilm was inhibited, and the nicotine concentration was decreased after adding mekramine, and the promoting effect of nicotine on the biofilm of two bacteria was also observed.
【作者單位】： 口腔疾病研究國家重點(diǎn)實(shí)驗(yàn)室國家口腔疾病臨床研究中心四川大學(xué)華西口腔醫(yī)院牙體牙髓病科;
【基金】：國家自然科學(xué)基金(81400501) 口腔疾病研究國家重點(diǎn)實(shí)驗(yàn)室專項經(jīng)費(fèi)(SKLOD201525) 四川大學(xué)大學(xué)生創(chuàng)新訓(xùn)練計劃(201-40298)~~
【分類號】：R781.4

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