本文選題：牙周炎　切入點(diǎn)：炎癥　出處：《大連醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：牙周病是一種慢性感染性疾病，一系列的免疫炎癥反應(yīng)參與了疾病的病理過程。細(xì)胞因子在免疫應(yīng)答和炎癥反應(yīng)中發(fā)揮重要作用，趨化因子是細(xì)胞因子家族中的重要成員。CXCL16是一種新型的趨化因子，通過其受體CXCR6參與炎性細(xì)胞向病變組織的趨化。本實(shí)驗(yàn)通過免疫組織化學(xué)法檢測(cè)趨化因子CXCL16及其受體CXCR6在中重度牙周炎齦組織和健康牙齦組織中的表達(dá)情況及其相互關(guān)系。 方法：收集遼寧省人民醫(yī)院口腔科門診就診和住院患者的牙齦組織，年齡控制在35～74周歲，將其分為兩組：健康牙齦組（40例）、中重度牙周炎組（39例）。 健康牙齦組納入標(biāo)準(zhǔn)：牙齒清潔，無(wú)齲洞，無(wú)疼痛感；牙齦顏色正常，無(wú)自發(fā)性或探診出血。中重度牙周炎組入選標(biāo)準(zhǔn)參照第四版《牙周病學(xué)》。 排除標(biāo)準(zhǔn)：有其他全身系統(tǒng)性疾病及藥物過敏史；3個(gè)月內(nèi)曾使用抗生素、腎上腺皮質(zhì)激素、非甾體類抗炎藥、性激素及避孕藥等；6個(gè)月內(nèi)有心梗發(fā)生；吸煙史。 按照免疫組織化學(xué)染色法制片，光學(xué)生物顯微鏡下觀察牙齦組織中CXCL16和CXCR6的分布和細(xì)胞定位，用免疫組織化學(xué)半定量分析(傳統(tǒng)人工計(jì)數(shù))法對(duì)數(shù)據(jù)進(jìn)行綜合評(píng)估，并使用SPSS15.0軟件包對(duì)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果：1. CXCL16及CXCR6在中重度牙周炎患者的牙齦組織中均有表達(dá)。CXCL16主要表達(dá)在結(jié)締組織區(qū)牙齦成纖維細(xì)胞、淋巴細(xì)胞（漿細(xì)胞）和血管內(nèi)皮細(xì)胞的細(xì)胞膜及細(xì)胞質(zhì)中，染色呈淡黃色至棕褐色；CXCR6主要表達(dá)在結(jié)締組織區(qū)牙齦成纖維細(xì)胞、淋巴細(xì)胞（漿細(xì)胞）和血管內(nèi)皮細(xì)胞的細(xì)胞膜上，染色呈淡黃色至棕褐色。 2. CXCL16在健康牙齦組織中無(wú)染色或散在淡黃色顆粒狀染色，CXCR6在正常牙齦組織中未見或偶見淡黃色顆粒狀染色。 3. CXCLl6在牙周炎齦組織、健康牙齦組織中的陽(yáng)性表達(dá)率分別是92.31%、40.00%，，在牙周炎中以陽(yáng)性表達(dá)為主，在健康牙齦組織中則低表達(dá)或無(wú)表達(dá)。中重度牙周炎組與健康牙齦組比較，CXCL16表達(dá)顯著性升高，差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。 4. CXCR6在牙周炎齦組織、健康牙齦組織中的陽(yáng)性表達(dá)率分別是100%、30.00%；中重度牙周炎組與健康牙齦組比較，CXCR6表達(dá)顯著性升高，差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。 5．經(jīng)相關(guān)性分析，CXCLl6與CXCR6在中重度牙周炎患者牙齦組織中的表達(dá)呈正相關(guān)(r=0.580，P0.05)，有統(tǒng)計(jì)學(xué)意義；而在健康牙齦組織中，二者的表達(dá)不相關(guān)。 結(jié)論：1.趨化因子CXCLl6及其受體CXCR6在中重度牙周炎組中的表達(dá)高于健康牙齦組，提示CXCLl6、CXCR6可能與牙周炎的發(fā)病有關(guān)。 2．CXCLl6和CXCR6在中重度牙周炎齦組織中的表達(dá)呈正相關(guān)，提示二者在牙周炎的發(fā)病過程中可能共同發(fā)揮作用。
[Abstract]:Objective : To investigate the expression of chemokine CXCL16 and its receptor on the gingival tissues and healthy gingival tissues of moderate and severe periodontitis by immunohistochemical method . CXCL16 plays an important role in immune response and inflammatory response .

Methods : The gingival tissues of the outpatient department and the hospitalized patients were collected from the Department of Stomatology of the People ' s Hospital of Liaoning Province . The age was controlled between 35 and 74 years , and the patients were divided into two groups : healthy gum group ( 40 cases ) and moderate and severe periodontitis group ( 39 cases ) .

Healthy gingival group included standard : tooth cleaning , no caries hole , no pain feeling ;
The gingival color is normal without spontaneous or diagnostic bleeding . The criteria for inclusion in the moderate and severe periodontitis group are referred to in the 4th edition of the periodontal disease group .

Exclusion criteria : there are other systemic diseases and drug allergies ;
antibiotics , adrenocorticoids , non - steroidal anti - inflammatory drugs , sex hormones and contraceptives have been used within 3 months ;
myocardial infarction occurred within 6 months ;
History of smoking .

According to the immunohistochemical staining method , the distribution and cell localization of CXCL16 and CXCR4 6 in gingival tissues were observed under the optical biological microscope . The data were comprehensively evaluated by semi - quantitative analysis ( traditional manual counting ) method , and the data were analyzed statistically by SPSS 15.0 .

Results : 1 . CXCL16 was expressed in gingival fibroblasts , lymphocytes ( plasma cells ) and vascular endothelial cells of connective tissue . CXCL16 was mainly expressed in gingival fibroblasts , lymphocytes ( plasma cells ) and vascular endothelial cells .
On the cell membranes of gingival fibroblasts , lymphocytes ( plasma cells ) and vascular endothelial cells in connective tissue , the staining was pale yellow to tan .

2 . The CXCL16 did not stain or disperse in pale yellow granular staining in healthy gum tissue .

3 . The positive expression rates of CXCLl6 in gingival tissues and healthy gums were 92.31 % and 40.00 % , respectively .

4 . The positive expression rates were 100 % and 30.00 % in the gingival tissues of periodontitis and healthy gum tissues , respectively .
Compared with the healthy gum group in moderate and severe periodontitis group , there was a statistically significant difference in the expression of IL 6 in the patients with moderate and severe periodontitis ( P0.05 ) .

5 . The correlation analysis showed that CXCLl6 had positive correlation between CXCLl6 ( r = 0.580 and P0.05 ) in the gingival tissues of patients with moderate and severe periodontitis ( r = 0.580 , P0.05 ) .
In healthy gum tissue , the expression of the two is not related .

Conclusion : 1 . The expression of chemokine CXCLl6 and its receptor in moderate and severe periodontitis group is higher than that of healthy gum group , and suggests CXCLl6 and CXCLl6 may be related to the pathogenesis of periodontitis .

2 . The expression of CXCLl6 and CD6 in gingival tissues of moderate and severe periodontitis was positively correlated , suggesting that both might play a role in the pathogenesis of periodontitis .

【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R781.42

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 孫琳;劉蕊;劉湘源;趙金霞;徐寧;龍麗;栗占國(guó);;類風(fēng)濕關(guān)節(jié)炎患者外周血CXCL16的表達(dá)及臨床意義[J];北京大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2010年05期

2 司京玉,聶生東,陳瑛,黃永鋒,顧順德,章魯;免疫組織化學(xué)顯微圖像分析技術(shù)進(jìn)展[J];國(guó)外醫(yī)學(xué).生物醫(yī)學(xué)工程分冊(cè);2002年03期

3 孫琳;劉湘源;趙金霞;滿斯亮;張霞;;Raf/MEK/ERK信號(hào)通路對(duì)CXCL16誘導(dǎo)的CIA淋巴細(xì)胞增殖的影響[J];軍醫(yī)進(jìn)修學(xué)院學(xué)報(bào);2010年11期

4 黃煜;李大金;;人早孕滋養(yǎng)細(xì)胞分泌趨化因子CXCL16促進(jìn)自身增殖和侵襲[J];分子細(xì)胞生物學(xué)報(bào);2006年01期

5 劉芳;趙彤;;CXCL16/CXCR6與炎癥性疾病[J];國(guó)際病理科學(xué)與臨床雜志;2010年05期

6 莊宇;簡(jiǎn)怡娜;譚志明;;趨化因子CXCL16與系統(tǒng)性紅斑狼瘡的研究進(jìn)展[J];中外醫(yī)學(xué)研究;2012年06期

7 呂永青;卜培莉;;CXCL16與動(dòng)脈粥樣硬化的關(guān)系研究[J];中國(guó)實(shí)用醫(yī)藥;2009年09期

8 周珊珊;魏巖;鄭楊;;PI3K/AKT/FKHRL1信號(hào)通路對(duì)CXLC16誘導(dǎo)的平滑肌細(xì)胞增殖的影響[J];中國(guó)實(shí)驗(yàn)診斷學(xué);2008年04期

本文編號(hào)：1668116