發(fā)布時間：2018-03-26 13:50

  本文選題：牙周膜　切入點：成纖維細胞　出處：《重慶醫(yī)學》2017年23期

【摘要】：目的研究白細胞介素17(IL-17)水平對人牙周膜成纖維細胞(HPDLF)中細胞核因子κB受體活化因子配體(RANKL)、骨保護素(OPG)表達的影響,探討IL-17與正畸牙根吸收的相關性。方法采用組織塊培養(yǎng)法,在體外建立人牙周膜成纖維細胞系。以不同水平(0、5、10、20、40ng/mL)的IL-17作用HPDLF 24h。采用RT-PCR和ELISA法,分別檢測RANKL和OPG的mRNA、蛋白的表達。結果 (1)在0ng/mL組中,HPDLF表達RANKL、OPG。(2)0~20ng/mL各組中,HPDLF中RANKL的mRNA、蛋白表達量與IL-17水平呈正相關性。(3)5~20ng/mL各組中,OPG的mRNA、蛋白表達量與IL-17水平呈負相關性。(4)0~20ng/mL組中,RANKL/OPG的mRNA、蛋白的比值與IL-17水平呈正相關性。結論在無IL-17刺激時,HPDLF可表達RANKL、OPG。IL-17能夠促進HPDLF表達RANKL,同時抑制HPDLF表達OPG,上調RANKL/OPG的比值。
[Abstract]:Objective to study the effect of interleukin 17 (IL 17) on the expression of nuclear factor 魏 B receptor activating factor ligand RANKL L (osteoprotegerin) in human periodontal ligament fibroblasts (HPD LF), and to explore the relationship between IL-17 and orthodontic root resorption. A human periodontal ligament fibroblast cell line was established in vitro. The HPDLF was treated with IL-17 at different levels (51010ng / mL) for 24 h. RT-PCR and ELISA methods were used. MRNAs and protein expressions of RANKL and OPG were detected respectively in 0ng/mL group. Results 1) in 0ng/mL group, the expression of RANKL in IL-17 was positively correlated with IL-17 level, and there was a negative correlation between protein expression and IL-17 level. The ratio of mRNA and protein of RANKL / OPG in 20 ng / mL group was positively correlated with the level of IL-17. Conclusion in the absence of IL-17 stimulation, the expression of RANKL / OPG.IL-17 can promote the expression of RANKL, inhibit the expression of HPDLF and up-regulate the ratio of RANKL/OPG.
【作者單位】： 廣西醫(yī)科大學附屬口腔醫(yī)院正畸科;
【基金】：國家自然科學基金資助項目(81360170)
【分類號】：R783.5

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本文編號：1668119