本文選題：口腔扁平苔蘚　切入點(diǎn)：雙向熒光差異凝膠電泳　出處：《山東醫(yī)藥》2016年16期 　論文類型：期刊論文

【摘要】：目的探討口腔扁平苔蘚(OLP)患者血清差異蛋白表達(dá)及意義。方法選擇6例OLP患者(觀察組)和6例健康志愿者(對(duì)照組),抽取空腹靜脈血后采用苯酚抽提法提取血清蛋白,Bradford法測(cè)定蛋白含量,雙向熒光差異凝膠電泳法分離檢測(cè)差異蛋白點(diǎn),液相色譜-質(zhì)譜聯(lián)用技術(shù)鑒定差異蛋白。選擇其中研究較少的蛋白,采用Western blotting法檢測(cè)兩組蛋白相對(duì)表達(dá)量。結(jié)果兩組血清差異蛋白點(diǎn)(583±183)個(gè),其中重復(fù)性好的蛋白差異點(diǎn)20個(gè),質(zhì)譜分析蛋白序列鑒定出12種蛋白質(zhì)。12種蛋白質(zhì)中,7種(補(bǔ)體C3、激肽原、α-2-巨球蛋白、人抗凝血酶Ⅲ、分泌型Ig A、補(bǔ)體C9、β-1-金屬結(jié)合球蛋白)在觀察組血清中表達(dá)降低,5種(結(jié)合珠蛋白、銅藍(lán)蛋白、載脂蛋白AⅠ、維生素D、人類因子B)表達(dá)升高。觀察組血清補(bǔ)體C3和人抗凝血酶Ⅲ蛋白相對(duì)表達(dá)量均低于對(duì)照組,載脂蛋白AⅠ高于對(duì)照組(P均0.01)。結(jié)論 OLP患者血清中存在12種差異蛋白質(zhì),血清補(bǔ)體C3和人抗凝血酶Ⅲ蛋白表達(dá)均降低,載脂蛋白AⅠ表達(dá)升高,其發(fā)病可能與免疫功能降低及感染有關(guān)。
[Abstract]:Objective to investigate the expression and significance of serum differential protein in patients with oral lichen planus (OLP). Methods six patients with OLP (observation group) and six healthy volunteers (control group) were selected to extract blood by phenol extraction after extracting fasting venous blood. The protein content was determined by Bradford method. Two-dimensional fluorescence differential gel electrophoresis was used to isolate and detect differential protein spots, and liquid chromatography-mass spectrometry was used to identify the differential proteins. Western blotting method was used to detect the relative expression of two groups of proteins. Results there were 583 鹵183 differential protein spots in the serum of the two groups, 20 of which had good reproducibility. Seven of 12 proteins were identified by mass spectrometry (complement C3, kallikinogen, 偽 -2-macroglobulin, human antithrombin 鈪，

本文編號(hào)：1575478