本文關(guān)鍵詞： 氧化苦參堿 內(nèi)毒素類 牙齦/微生物學(xué) 擬桿菌屬 牙周膜/細(xì)胞學(xué) 堿性磷酸酶　出處：《福建醫(yī)科大學(xué)學(xué)報(bào)》2017年03期 　論文類型：期刊論文

【摘要】：目的研究氧化苦參堿對(duì)牙齦卟啉菌內(nèi)毒素(Pg-LPS)作用下人牙周膜細(xì)胞(PDLC)堿性磷酸酶(ALP)的活性及其表達(dá)的影響。方法采用細(xì)胞培養(yǎng)技術(shù)分離PDLC,對(duì)照組為僅含1%胎牛血清的細(xì)胞培養(yǎng)液,實(shí)驗(yàn)組分別加入不同濃度的氧化苦參堿溶液和Pg-LPS。采用細(xì)胞培養(yǎng)技術(shù)和酶聯(lián)免疫、實(shí)時(shí)熒光定量RT-PCR技術(shù),觀察Pg-LPS對(duì)PDLC的ALP活性及其表達(dá)的影響。結(jié)果在25μg/mL的Pg-LPS作用下,PDLC的ALP活性及其表達(dá)明顯減低,加入一定濃度的氧化苦參堿溶液后,PDLC的ALP活性及其表達(dá)有一定程度恢復(fù)。結(jié)論氧化苦參堿可以減輕Pg-LPS對(duì)PDLC的破壞,對(duì)Pg-LPS抑制PDLC的ALP活性及其表達(dá)的現(xiàn)象具有拮抗作用。
[Abstract]:Objective to study the effect of oxymatrine on the activity and expression of alkaline phosphatase (ALP) in human periodontal ligament cells (PDLC) induced by Pg-LPS. methods PDLC was isolated by cell culture technique, and the control group was used as a cell culture medium containing only 1% fetal bovine serum. In the experimental group, different concentrations of oxymatrine solution and Pg-LPSS were added respectively. Cell culture technique and enzyme-linked immunosorbent assay (Elisa) were used, and real-time fluorescence quantitative RT-PCR technique was used. To observe the effect of Pg-LPS on the ALP activity and expression of PDLC. Results the ALP activity and expression of PDLC were significantly decreased under the treatment of 25 渭 g / mL Pg-LPS. Conclusion Oxymatrine can attenuate the destruction of PDLC by Pg-LPS and inhibit the ALP activity and expression of PDLC by Pg-LPS.
【作者單位】： 福建醫(yī)科大學(xué)附屬第一醫(yī)院口腔科;
【基金】：福建省自然科學(xué)基金(2017J05124) 中華口腔醫(yī)學(xué)會(huì)口腔疾病與全身疾病關(guān)系研究項(xiàng)目(CSA-Y2015-09)
【分類號(hào)】：R781.4
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本文編號(hào)：1548512