【摘要】：目的:建立AD(Alzheimer disease)小鼠動物模型,研究癸酸(DA)對其影響作用。方法:將50只雄性昆明種小鼠隨機分為正常組,模型組,低劑量給藥組(DA 50mg/kg),中劑量給藥組(DA 100mg/kg),和高劑量給藥組(DA 200mg/kg)共5個實驗組。正常組小鼠頸背部注射生理鹽水,模型組和癸酸給藥組頸背部皮下均注射D-半乳糖溶液。低劑量給藥組,中劑量給藥組,和高劑量給藥組小鼠皮下注射癸酸。8周后觀察小鼠行為學變化,取小鼠腦組織觀察腦神經(jīng)元病理學變化,檢測腦組織中PPARγ、NFκB蛋白含量以及檢測血清中TNF-α、i NOS因子的水平。結(jié)果:水迷宮測試中癸酸各劑量給藥組小鼠學習記憶能力提高;病理學結(jié)果顯示癸酸給藥后神經(jīng)細胞損傷程度減弱,均低于模型組;小鼠腦組織中PPARγ,NFκB,以及血清中TNF-α、i NOS的含量均低于模型組。以高劑量給藥組最為顯著。結(jié)論:癸酸可以改善AD模型小鼠的一定程度的學習記憶能力,減輕神經(jīng)元損傷程度,降低AD小鼠某些炎性成分表達,其作用機制可能通過激動PPAR-γ表達減少NFκB、TNF-α、i NOS的生成有關。
[Abstract]:Objective: to establish an animal model of AD (Alzheimer disease) mice and study the effect of decanoic acid (DA) on it. Methods: fifty male Kunming mice were randomly divided into five experimental groups: normal group, model group, low dose administration group (DA 50mg/kg), middle dose administration group (DA 100mg/kg) and high dose administration group (DA 200mg/kg). Normal saline was injected into the neck and back of the normal group, and D-galactose solution was injected subcutaneously into the neck and back of the model group and decanoic acid group. The mice in the low dose administration group, the middle dose administration group and the high dose administration group were subcutaneously injected with decanoic acid. After 8 weeks, the behavioral changes of the mice were observed, the pathological changes of brain neurons were observed in the brain tissue of the mice, and the PPAR 緯 in the brain tissue was detected. The content of NF kappa B protein and the level of TNF- 偽, i NOS factor in serum were detected. Results: in the water maze test, the learning and memory ability of the mice in each dose of decanoic acid group was improved, and the pathological results showed that the degree of nerve cell injury decreased after decanoic acid administration, which was lower than that in the model group. The contents of PPAR 緯, NF kappa B and TNF- 偽, i NOS in brain tissue of mice were lower than those in model group. The high dose group was the most significant. Conclusion: decanoic acid can improve the learning and memory ability of AD model mice, reduce the degree of neuronal injury and decrease the expression of some inflammatory components in AD mice. The mechanism of decanoic acid may decrease NF kappa B, TNF偽 by activating the expression of PPAR- 緯. The generation of I NOS is related.
【學位授予單位】：桂林醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R749.16

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