發(fā)布時(shí)間：2019-04-25 11:57

【摘要】：目的通過(guò)觀察血管性癡呆(VD)大鼠模型學(xué)習(xí)記憶能力的改變,以VD大鼠大腦神經(jīng)血管單元為觀察靶點(diǎn),運(yùn)用磁共振波譜技術(shù)分別檢測(cè)VD病程中1、2、3、4周海馬區(qū)神經(jīng)元、星形膠質(zhì)細(xì)胞的代謝,進(jìn)而闡明腦通湯治療血管性癡呆的相關(guān)作用機(jī)制。 方法SD大鼠60只隨機(jī)分為4組：①假手術(shù)組②模型組③多奈哌齊組④腦通湯組,每組15只。經(jīng)Morris水迷宮檢測(cè)篩選后,后3組合格大鼠采用改良的4血管阻斷法(4-VO)進(jìn)行造模。造模后進(jìn)行灌胃治療,每日1次,共4周。分別于造模后1、2、3、4周運(yùn)用磁共振波譜技術(shù)檢測(cè)大鼠VD病程中海馬區(qū)神經(jīng)元、星形膠質(zhì)細(xì)胞的代謝改變 結(jié)果模型組NAA/Cr比值降低,Cho/Cr比值增高,相對(duì)于假手術(shù)組來(lái)說(shuō),具有統(tǒng)計(jì)學(xué)意義(PO.O1～P0.05),這表明經(jīng)過(guò)VD造模后使大鼠大腦海馬區(qū)代謝物發(fā)生改變,即神經(jīng)元活力降低以及膠質(zhì)細(xì)胞過(guò)度增生。腦通湯組能顯著提高大腦海馬區(qū)NAA/Cr的含量、降低Cho/Cr比值,與模型組相比具有統(tǒng)計(jì)學(xué)意義(P0.01-P0.05),說(shuō)明此方具有較好的改善神經(jīng)元的受損或丟失并抑制膠質(zhì)細(xì)胞過(guò)度增生的作用,對(duì)血管性癡呆的發(fā)生和發(fā)展有較好的延緩和改善的療效,且這種作用優(yōu)于鹽酸多奈哌齊,是腦通湯在臨床中改善VD病人學(xué)習(xí)記憶能力的干預(yù)因素和治療機(jī)制之一。 結(jié)論改良的四血管阻斷法(4-VO)制作的VD模型表現(xiàn)為學(xué)習(xí)記憶功能下降,該模型可用作血管性癡呆動(dòng)物模型。腦通湯組具有較好的改善神經(jīng)元的受損或丟失并抑制膠質(zhì)細(xì)胞過(guò)度增生的作用,對(duì)血管性癡呆的發(fā)生和發(fā)展有較好的延緩和改善的療效,且這種作用優(yōu)于鹽酸多奈哌齊。
[Abstract]:Objective to observe the changes of learning and memory ability in (VD) rats with vascular dementia (VD), and to detect the hippocampal neurons in the course of VD by using magnetic resonance spectroscopy (Mrs) at 1, 2, 3, 4 weeks, respectively, taking the neurovascular unit of the brain of VD rats as the target. The metabolism of astrocytes further elucidates the mechanism of Naotong decoction in the treatment of vascular dementia. Methods Sixty SD rats were randomly divided into 4 groups: 1 sham operation group 2 model group 3 Donepezil group 4 Naotong decoction group with 15 rats in each group. After screening by Morris water maze test, the model was made by modified 4-vessel occlusion (4-VO) method in the latter 3 groups. After the model was made, the rats were treated by gastric perfusion once a day for a total of 4 weeks. The metabolic changes of hippocampal neurons and astrocytes in rats during the course of VD were detected by magnetic resonance spectroscopy at 1, 2, 3, 4 weeks after the establishment of the model. The results showed that the ratio of NAA/Cr to Cho/Cr in the model group was lower and the ratio of astrocytes was higher than that in the model group. Compared with the sham-operated group, it was statistically significant (PO.O1~P0.05), which indicated that after VD modeling, the metabolites in the hippocampus of rats were changed, that is, the activity of neurons was decreased and the glial cells were over-proliferated. Naotong decoction group can significantly increase the content of NAA/Cr in the hippocampus and decrease the ratio of Cho/Cr, which has statistical significance (P0.01-P0.05) compared with the model group. It shows that this prescription has a better effect on improving the damage or loss of neurons and inhibiting the excessive proliferation of glial cells, and has a better effect on delaying and improving the occurrence and development of vascular dementia, and this effect is better than that of Donepezil Hydrochloride. It is one of the intervention factors and therapeutic mechanism of Naotong decoction to improve the learning and memory ability of VD patients. Conclusion the improved four-vessel occlusion (4-VO)-induced VD model is characterized by learning and memory impairment, and can be used as an animal model of vascular dementia. Naotong decoction group had a better effect on improving the damage or loss of neurons and inhibiting the excessive proliferation of glial cells, and had a better effect on delaying and improving the occurrence and development of vascular dementia, and this effect was better than that of Donepezil Hydrochloride.
【學(xué)位授予單位】：貴陽(yáng)中醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R445.2;R749.13

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