【摘要】：為了研究慢性應(yīng)激性抑郁發(fā)生中海馬轉(zhuǎn)化生長因子β1(transforming growth factor beta 1,TGF-β1)和硒蛋白P的作用及其關(guān)系,實(shí)驗(yàn)通過建立慢性不可預(yù)見性溫和應(yīng)激(Chronic Unpredictable Mild Stress,CUMS)動(dòng)物抑郁模型,同時(shí)海馬內(nèi)微量注射TGF-β1以及TGF-β1型受體激酶抑制劑LY-364947,然后測量大鼠體重變化率,并通過糖水消耗以及曠場實(shí)驗(yàn)、懸尾實(shí)驗(yàn)檢測大鼠行為變化,運(yùn)用Elisa、Western blot分別檢測大鼠海馬組織內(nèi)TGF-β1和硒蛋白P的含量變化.結(jié)果顯示,與正常對(duì)照組相比,CUMS組大鼠表現(xiàn)出明顯的抑郁樣行為,海馬組織內(nèi)TGF-β1含量升高,硒蛋白P表達(dá)下降;正常大鼠海馬內(nèi)注射TGF-β1并不導(dǎo)致抑郁樣行為,相反,CUMS同時(shí)海馬內(nèi)注射TGF-β1能逆轉(zhuǎn)應(yīng)激導(dǎo)致的抑郁樣行為;正常和CUMS大鼠海馬注射TGF-β1均能明顯抑制硒蛋白P的表達(dá);而應(yīng)激的同時(shí)注射TGF-β1型受體激酶抑制劑LY-364947同樣具有抗抑郁效應(yīng),此時(shí)硒蛋白P表達(dá)較CUMS組明顯升高.以上結(jié)果表明:海馬TGF-β1和硒蛋白P參與了應(yīng)激性抑郁的發(fā)生;硒蛋白P對(duì)海馬可能具有保護(hù)作用,TGF-β1通過TGF-β1型受體抑制硒蛋白P表達(dá)可能是導(dǎo)致抑郁發(fā)生的途徑之一,而TGF-β1抗抑郁作用可能是經(jīng)過TGF-β1型受體以外的其他途徑實(shí)現(xiàn).
[Abstract]:In order to study the role of transforming growth factor-尾 1 (TGF-尾 1) and selenoprotein P in hippocampal transforming growth factor-尾 1 (TGF-尾 1) and selenoprotein P in the development of chronic stress depression, we established chronic unpredictable mild stress (Chronic Unpredictable Mild Stress,. CUMS) animal model of depression, and microinjection of TGF- 尾 1 and TGF- 尾 1 receptor kinase inhibitor LY-364947, into hippocampus, then measure the rate of body weight change in rats, and detect the behavior changes of rats by glucose consumption, open field test and tail suspension test. The contents of TGF- 尾 1 and selenoprotein P in hippocampus of rats were measured by Elisa,Western blot. The results showed that compared with the normal control group, the rats in CUMS group showed obvious depression-like behavior, the content of TGF- 尾 1 in hippocampus increased and the expression of selenoprotein P decreased. Injection of TGF- 尾 1 into normal rat hippocampus did not lead to depression-like behavior, on the contrary, CUMS and TGF- 尾 1 injection into hippocampus could reverse depression-like behavior induced by stress, while injection of TGF- 尾 1 into hippocampus of normal and CUMS rats could significantly inhibit the expression of selenoprotein P. LY-364947, an inhibitor of TGF- 尾 type 1 receptor kinase, also had antidepressant effect at the same time, and the expression of selenoprotein P was significantly higher than that of CUMS group. The results indicated that TGF- 尾 1 and selenoprotein P in hippocampus were involved in the occurrence of stress depression. Selenoprotein P may have protective effect on hippocampus. The inhibition of selenoprotein P expression by TGF- 尾 1 through TGF- 尾 1 receptor may be one of the pathways leading to depression. The antidepressant effect of TGF- 尾 1 may be achieved through other than TGF- 尾 1 receptor.
【作者單位】： 陜西師范大學(xué)生命科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(81271488) 中央高校基本科研業(yè)務(wù)費(fèi)專項(xiàng)資金項(xiàng)目(GK200901011)
【分類號(hào)】：R749.4

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1 李菲;慢性應(yīng)激性抑郁發(fā)生中轉(zhuǎn)化生長因子-β1與硒蛋白P的作用及其關(guān)系[D];陜西師范大學(xué);2013年

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