【摘要】：目的探討創(chuàng)傷后應(yīng)激障礙(posttraumatic stress disorder,PTSD)大鼠行為學(xué)改變及腦內(nèi)胱硫醚-β-合成酶(cysta-thionine-β-synthase,CBS)/硫化氫(hydrogen sulfide,H_2S)水平的變化,并研究外源性補(bǔ)充H_2S對大鼠行為學(xué)影響的作用及機(jī)制。方法應(yīng)用連續(xù)單一應(yīng)激方案制備創(chuàng)傷后應(yīng)激障礙動(dòng)物模型,用Morris水迷宮檢測大鼠空間學(xué)習(xí)記憶能力,Western blot技術(shù)和亞甲基藍(lán)法檢測海馬腦區(qū)CBS/H_2S體系含量,用多管微電極在體細(xì)胞外電生理記錄海馬神經(jīng)元放電情況。結(jié)果 (1)與正常對照組比較,PTSD組大鼠逃避潛伏期增加(P0.01),在平臺所在象限停留時(shí)間百分比和通過原平臺位置次數(shù)明顯減少(P0.01);海馬腦區(qū)的CBS/H_2S體系含量明顯降低(P0.01,P0.05)。(2)與PTSD組比較,PTSD+硫氫化鈉(sodium hydrosulfide,NaH S)組大鼠逃避潛伏期減小(P0.01),在平臺所在象限停留時(shí)間百分比和通過原平臺位置次數(shù)明顯增加(P0.01)。(3)微量壓力注射L-半胱氨酸(L-cysteine)使海馬神經(jīng)元自發(fā)放電頻率明顯升高(P㩳0.01)。結(jié)論P(yáng)TSD大鼠通過抑制海馬腦區(qū)CBS/H_2S體系導(dǎo)致空間學(xué)習(xí)記憶能力降低。H_2S改善PTSD大鼠空間學(xué)習(xí)記憶能力的機(jī)制可能與其增加海馬神經(jīng)元放電頻率和興奮性有關(guān)。海馬腦區(qū)CBS/H_2S體系導(dǎo)致空間學(xué)習(xí)記憶能力降低。H_2S改善PTSD大鼠空間學(xué)習(xí)記憶能力的機(jī)制可能與其增加海馬神經(jīng)元放電頻率和興奮性有關(guān)。
[Abstract]:Objective to investigate the behavioral changes and the changes of cysta-thionine- 尾 -synthase (cysta-thionine- 尾-synthase,CBS) / hydrogen sulfide (hydrogen sulfide,H_2S) levels in the brain of rats with post-traumatic stress disorder (posttraumatic stress disorder,PTSD). The effect and mechanism of exogenous H 2S supplementation on behavior of rats were studied. Methods Morris water maze was used to detect the spatial learning and memory ability of rats, and methylene blue method was used to detect the content of CBS/H_2S system in hippocampus. The discharge of hippocampal neurons was recorded by multitube microelectrode in external somatic electrophysiology. Results (1) compared with the normal control group, the escape latency of PTSD rats increased (P0.01), the percentage of staying time in the quadrant of the platform and the number of times passing through the original platform decreased significantly (P0.01). Compared with PTSD group, the content of CBS/H_2S system in hippocampus decreased significantly (P0.01). (2), and the escape latency of PTSD group (sodium hydrosulfide,NaH S) decreased (P0.01). The percentage of residence time in the quadrant of the platform and the number of times through the original platform position increased significantly (P0.01). (3). Microinjection of L-cysteine (L-cysteine) significantly increased the spontaneous discharge frequency of hippocampal neurons. (P0. 01). Conclusion the ability of spatial learning and memory in PTSD rats is reduced by inhibiting the CBS/H_2S system in hippocampal area. The mechanism of H2S improving spatial learning and memory ability of PTSD rats may be related to the increase of firing frequency and excitability of hippocampal neurons. The ability of spatial learning and memory was decreased by CBS/H_2S system in hippocampal area. The mechanism of H2S improving spatial learning and memory ability of PTSD rats may be related to the increase of firing frequency and excitability of hippocampal neurons.
【作者單位】： 濱州醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)院生理學(xué)教研室;濱州醫(yī)學(xué)院附屬醫(yī)院院辦;濱州醫(yī)學(xué)院醫(yī)藥研究中心;濱州醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)院生物化學(xué)教研室;濱州醫(yī)學(xué)院藥理學(xué)教研室;
【基金】：山東省自然科學(xué)基金資助項(xiàng)目(NoZR2013HL009,ZR2013HL002,ZR2013HL003,ZR2013HL007) 煙臺市科技發(fā)展計(jì)劃項(xiàng)目(No2012ZH248) 山東省高�？萍加�(jì)劃項(xiàng)目(NoJ12LM03)
【分類號】：R749.5

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