【摘要】：目的通過對(duì)比觀察AD模型小鼠海馬與小腦Aβ沉積和相關(guān)miRNAs表達(dá)的變化,探討AD模型小鼠小腦是否存在Aβ沉積和相關(guān)miRNAs的差異表達(dá)。方法選擇12月齡大小的APPswe/PS△E9雙轉(zhuǎn)基因AD小鼠作為實(shí)驗(yàn)組(EG),同月齡的同種系的野生型小鼠C57為對(duì)照組(CG),每組各12只。將小鼠腦組織分為左右兩部分,右側(cè)腦組織行剛果紅染色檢測(cè)淀粉樣物質(zhì)在海馬及小腦的沉積;左側(cè)腦組織分別用于提取海馬及小腦的miRNA,應(yīng)用實(shí)時(shí)熒光定量PCR方法分別檢測(cè)兩組小鼠海馬及小腦組織的miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表達(dá)情況。結(jié)果剛果紅染色:實(shí)驗(yàn)組小鼠海馬及小腦均可見橘紅色的Aβ沉積,對(duì)照組小鼠海馬及小腦均未見橘紅色的Aβ沉積。實(shí)時(shí)熒光定量PCR:四種miRNAs在實(shí)驗(yàn)組海馬的表達(dá)均低于對(duì)照組(P0.05);miRNA-135a-5p、miRNA-298-5p和miR-669f-3p在實(shí)驗(yàn)組小腦的表達(dá)低于對(duì)照組(P0.05);miRNA-298-5p和miR-669f-3p在實(shí)驗(yàn)組海馬的表達(dá)低于小腦(P0.05)。結(jié)論 APPswe/PS△E9雙轉(zhuǎn)基因AD小鼠小腦中也存在Aβ沉積,其形成可能與miRNA-135a-5p、miRNA-298-5p和miR-669f-3p的表達(dá)下調(diào)有關(guān)。
[Abstract]:Objective to observe the changes of A 尾 deposition and related miRNAs expression in hippocampus and cerebellum of AD model mice, and to explore whether there is a difference between A 尾 deposition and related miRNAs expression in cerebellum of AD model mice. Methods 12 months old APPswe/PS E9 double transgenic AD mice were selected as experimental group (EG), homologous C57 mice of the same month age as control group, 12 mice in each group were selected as control group. The brain tissue of mice was divided into two parts: the right brain was stained with Congo red to detect the deposition of amyloid in hippocampus and cerebellum. The expression of miRNA-135a-5p,miRNA-298-5p,miRNA-466b-3p and miR-669f-3p in hippocampus and cerebellum of the two groups were detected by real-time fluorescence quantitative PCR method. Results Congo red staining: orange A 尾 was found in hippocampus and cerebellum of mice in experimental group, but not in hippocampus and cerebellum in control group. The expression of real-time quantitative PCR: miRNAs in hippocampus of experimental group was lower than that of control group (P0.05), and the expression of miRNA-135a-5p,miRNA-298-5p and miR-669f-3p in cerebellum of experimental group was lower than that of control group (P0.05). The expression of miRNA-298-5p and miR-669f-3p in hippocampus of experimental group was lower than that of cerebellum (P0.05). Conclusion there is also A 尾 deposition in cerebellum of APPswe/PS E9 double transgenic AD mice, which may be related to the down-regulation of miRNA-135a-5p,miRNA-298-5p and miR-669f-3p expression.
【作者單位】： 中南大學(xué)湘雅三醫(yī)院神經(jīng)內(nèi)科;
【基金】：湖南省科技廳社會(huì)發(fā)展支撐計(jì)劃課題(2012SK3218)
【分類號(hào)】：R749.16

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本文編號(hào)：2337567