【摘要】：目的觀察美滿霉素對阿爾茨海默病(Alzheimer's disease,AD)大鼠空間學習記憶和海馬長時程增強(long-term potentiation,LTP)以及N-甲基-D-天冬氨酸受體2B亞基(N-methyl-D-aspartate receptor subunit 2B,NR2B)表達的影響,探討美滿霉素對AD大鼠神經(jīng)保護的作用機制。方法選用健康雄性SD大鼠36只,隨機分為對照組、模型組、美滿霉素治療組,每組12只。通過側腦室注射Aβ25-35制作AD大鼠模型。Morris水迷宮檢測各組大鼠空間學習記憶能力;在體電生理法記錄各組大鼠海馬LTP的誘導情況;免疫熒光組織化學法檢測各組大鼠海馬NR2B的表達。結果與對照組相比,模型組平均逃避潛伏期和第一次到達原平臺的時間明顯延長,穿環(huán)次數(shù)明顯減少(P0.05),海馬LTP幅度明顯降低,NR2B表達明顯減少(P0.05);與模型組相比,美滿霉素治療組平均逃避潛伏期和第一次到達原平臺的時間明顯縮短,穿環(huán)次數(shù)明顯增加(P0.05),海馬LTP幅度明顯升高,NR2B表達明顯增加(P0.05)。結論美滿霉素可以通過促進海馬LTP的形成,上調NR2B的表達,改善AD大鼠認知功能障礙。
[Abstract]:Objective to observe the effects of minocycline on spatial learning and memory, hippocampal long-term potentiation (long-term potentiation,LTP) and the expression of N- methyl-Daspartic acid receptor 2B subunit (N-methyl-D-aspartate receptor subunit _ 2Bn NR2B) in rats with Alzheimer's disease (Alzheimer's disease,AD). To investigate the neuroprotective mechanism of minocycline on AD rats. Methods Thirty-six healthy male SD rats were randomly divided into control group, model group and minocycline treatment group with 12 rats in each group. The AD rat model was made by intracerebroventricular injection of A 尾 25-35. The spatial learning and memory ability of each group was measured by Morris water maze, the induction of hippocampal LTP in each group was recorded by electrophysiological method, and the expression of NR2B in hippocampus was detected by immunofluorescence histochemistry. Results compared with the control group, the average escape latency and the first time to reach the original platform in the model group were significantly prolonged, the number of annular piercing was significantly decreased (P0.05), the amplitude of LTP in hippocampus was significantly decreased (P0.05), and the expression of NR2B was significantly decreased in the model group (P0.05). In minocycline treatment group, the average escape latency and the first time to reach the original platform were significantly shortened, the number of annular piercing increased significantly (P0.05), and the amplitude of LTP in hippocampus increased significantly (P0.05), and the expression of NR2B in hippocampus increased significantly (P0.05). Conclusion minocycline can improve the cognitive function of AD rats by promoting the formation of LTP and up-regulating the expression of NR2B.
【作者單位】： 漯河醫(yī)學高等�？茖W校;
【基金】：2015年度河南省科技廳基礎與前沿技術研究(152300410014) 2014年度河南省科技廳自然科學資助項目(142300410431) 漯河醫(yī)學高等�？茖W校2015年度基礎科學重點研究項目(2015-S-LMC06)
【分類號】：R749.16

【相似文獻】

相關期刊論文 前5條

1 蔡志友;晏勇;余昌胤;張駿;黃良國;晏寧;承歐梅;吳芳;李潔穎;趙宇;;美滿霉素增強血管性癡呆大鼠自由基清除[J];中國老年學雜志;2009年03期

2 蔡志友;晏勇;孫善全;張駿;黃良國;晏寧;吳芳;李潔穎;;美滿霉素改善血管性癡呆大鼠認知功能損傷并抑制氧化應激(英文)[J];Neuroscience Bulletin;2008年05期

3 蔡志友;晏勇;余昌胤;張駿;黃良國;;美滿霉素抑制GFAP與β-淀粉樣蛋白在糖尿病大鼠海馬CA3區(qū)的表達[J];中國新藥與臨床雜志;2009年02期

4 黃承紅;蔡志友;曾令瓊;彭亞利;;美滿霉素對血管性癡呆大鼠VEGF、IL-1β和TNF-α表達的影響[J];重慶醫(yī)學;2008年07期

5 蔡志友;晏寧;晏勇;承歐梅;余昌胤;張駿;楊麗;黃良國;詹劍;周妮;;美滿霉素抑制血管性癡呆大鼠腦組織NF-κB、GFAP與IL-1β的表達[J];第二軍醫(yī)大學學報;2008年10期

，

本文編號：2245942