【摘要】：目的觀察美滿霉素對(duì)阿爾茨海默病(Alzheimer's disease,AD)大鼠空間學(xué)習(xí)記憶和海馬長時(shí)程增強(qiáng)(long-term potentiation,LTP)以及N-甲基-D-天冬氨酸受體2B亞基(N-methyl-D-aspartate receptor subunit 2B,NR2B)表達(dá)的影響,探討美滿霉素對(duì)AD大鼠神經(jīng)保護(hù)的作用機(jī)制。方法選用健康雄性SD大鼠36只,隨機(jī)分為對(duì)照組、模型組、美滿霉素治療組,每組12只。通過側(cè)腦室注射Aβ25-35制作AD大鼠模型。Morris水迷宮檢測各組大鼠空間學(xué)習(xí)記憶能力;在體電生理法記錄各組大鼠海馬LTP的誘導(dǎo)情況;免疫熒光組織化學(xué)法檢測各組大鼠海馬NR2B的表達(dá)。結(jié)果與對(duì)照組相比,模型組平均逃避潛伏期和第一次到達(dá)原平臺(tái)的時(shí)間明顯延長,穿環(huán)次數(shù)明顯減少(P0.05),海馬LTP幅度明顯降低,NR2B表達(dá)明顯減少(P0.05);與模型組相比,美滿霉素治療組平均逃避潛伏期和第一次到達(dá)原平臺(tái)的時(shí)間明顯縮短,穿環(huán)次數(shù)明顯增加(P0.05),海馬LTP幅度明顯升高,NR2B表達(dá)明顯增加(P0.05)。結(jié)論美滿霉素可以通過促進(jìn)海馬LTP的形成,上調(diào)NR2B的表達(dá),改善AD大鼠認(rèn)知功能障礙。
[Abstract]:Objective to observe the effects of minocycline on spatial learning and memory, hippocampal long-term potentiation (long-term potentiation,LTP) and the expression of N- methyl-Daspartic acid receptor 2B subunit (N-methyl-D-aspartate receptor subunit _ 2Bn NR2B) in rats with Alzheimer's disease (Alzheimer's disease,AD). To investigate the neuroprotective mechanism of minocycline on AD rats. Methods Thirty-six healthy male SD rats were randomly divided into control group, model group and minocycline treatment group with 12 rats in each group. The AD rat model was made by intracerebroventricular injection of A 尾 25-35. The spatial learning and memory ability of each group was measured by Morris water maze, the induction of hippocampal LTP in each group was recorded by electrophysiological method, and the expression of NR2B in hippocampus was detected by immunofluorescence histochemistry. Results compared with the control group, the average escape latency and the first time to reach the original platform in the model group were significantly prolonged, the number of annular piercing was significantly decreased (P0.05), the amplitude of LTP in hippocampus was significantly decreased (P0.05), and the expression of NR2B was significantly decreased in the model group (P0.05). In minocycline treatment group, the average escape latency and the first time to reach the original platform were significantly shortened, the number of annular piercing increased significantly (P0.05), and the amplitude of LTP in hippocampus increased significantly (P0.05), and the expression of NR2B in hippocampus increased significantly (P0.05). Conclusion minocycline can improve the cognitive function of AD rats by promoting the formation of LTP and up-regulating the expression of NR2B.
【作者單位】： 漯河醫(yī)學(xué)高等�？茖W(xué)校;
【基金】：2015年度河南省科技廳基礎(chǔ)與前沿技術(shù)研究(152300410014) 2014年度河南省科技廳自然科學(xué)資助項(xiàng)目(142300410431) 漯河醫(yī)學(xué)高等專科學(xué)校2015年度基礎(chǔ)科學(xué)重點(diǎn)研究項(xiàng)目(2015-S-LMC06)
【分類號(hào)】：R749.16

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