【摘要】：目的探討免疫細(xì)胞膜表面整合素(CD11b)在β-淀粉樣蛋白(Aβ)所引起阿爾茨海默病(AD)中的作用、機(jī)制及與Aβ劑量的關(guān)系。方法雄性Wistar大鼠50只,隨機(jī)分為A、B、C、D、E 5組,每組10只;A組大鼠雙側(cè)海馬CA1區(qū)注射5μl生理鹽水,B~E組雙側(cè)海馬注射5μl Aβ(分別含Aβ_(25~35)0.5、1、5、10μg);HE染色觀察五組大鼠海馬CA1區(qū)細(xì)胞形態(tài)及數(shù)量變化;免疫組化(IHC)檢測海馬CA1區(qū)Aβ、CD11b表達(dá);實(shí)時(shí)熒光定量PCR技術(shù)(qRT-PCR)檢測CD11b mRNA表達(dá)。結(jié)果 HE染色,光鏡下觀察D組、E組大鼠海馬CA1區(qū)神經(jīng)細(xì)胞數(shù)明顯少于A組、B組、C組(P0.01);IHC檢測D組、E組兩組Aβ陽性表達(dá)率均高于A組、B組、C組(P0.01);D組、E組CD11b陽性表達(dá)率明顯高于A組、B組、C組(P0.01);qRT-PCR檢測大鼠海馬組織內(nèi)CD11b mRNA表達(dá)D組、E組組明顯高于A組、B組、C組(P0.01)。結(jié)論 Aβ可激活小膠質(zhì)細(xì)胞,募集MG到Aβ沉積部位,Aβ沉積量和CD11b表達(dá)量呈正相關(guān)。
[Abstract]:Objective to investigate the role and mechanism of immunomembrane integrin (CD11b) in (AD) of Alzheimer's disease induced by 尾 -amyloid protein (A 尾) and its relationship with A 尾 dose. Methods Fifty male Wistar rats were randomly divided into two groups: group A (n = 10) and group A (n = 10). In each group, 5 渭 l A 尾 (containing A 尾 _ (25) 35) 0.5A ~ (1 +) 510 渭 g was injected into the bilateral hippocampal CA1 area of rats in each group, and the morphological and quantitative changes of CA1 cells in the hippocampus of the five groups were observed by HE staining. Immunohistochemical (IHC) was used to detect the expression of A 尾 -CD11b in hippocampal CA1 and real-time quantitative PCR (qRT-PCR) was used to detect the expression of CD11b mRNA. Results HE staining, The number of CA1 neurons in the hippocampus of group D was significantly less than that of group A (P0.01). The positive expression rate of A 尾 in group D was higher than that in group C (P 0.01). The positive rate of CD11b in group E was significantly higher than that in group A (P 0.01). The expression of CD11b mRNA in hippocampus of group D was significantly higher than that of group C (P0.01). Conclusion A 尾 can activate microglia cells, and there is a positive correlation between the amount of A 尾 deposition and the expression of CD11b in recruiting MG to A 尾 deposition site.
【作者單位】： 承德護(hù)理職業(yè)學(xué)院;承德醫(yī)學(xué)院;
【基金】：河北省教育廳重點(diǎn)資助課題(No.ZD20131051) 河北省中醫(yī)藥抗癡呆重點(diǎn)研究室建設(shè)項(xiàng)目(No.20142803) 河北省高校重點(diǎn)學(xué)科資助項(xiàng)目(No.20130337)
【分類號(hào)】：R749.16

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