本文選題：阿爾茨海默病 + 聚集素　； 參考：《山西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：探討中國漢族人聚集素在阿爾茨海默中的含量和意義以及阿爾茨海默病(AD)與聚集素基因的多態(tài)性的關(guān)系。 方法： 1.收集AD組與對照組的病例。 2.兩組患者均取3ml外周靜脈血。 3.采用低滲溶血、常規(guī)酚－氯仿法提取白細(xì)胞基因組中的DNA。 4.應(yīng)用聚合酶鏈反應(yīng)-限制性片段長度多態(tài)性（PCR-RFLP）的技術(shù)觀察聚集素基因多態(tài)性。 5.采用酶聯(lián)免疫吸附測定法(ELISA)檢測AD患者（AD組）與健康老年人（對照組）血漿聚集素水平。 結(jié)果： 1.AD組聚集素基因C/C基因型、等位基因C分布明顯高于對照組(χ2=6.673,P0.05)（χ2=15.060，，P0.05)；聚集素等位基因C、基因型C/C與AD呈顯著正相關(guān)(OR=3.685，95%Cl=1.855-7.320)（OR=2.750，95%Cl=1.260-6.002）。 2.AD組血漿聚集素水平顯著高于對照組，差異具有統(tǒng)計學(xué)意義（P0.01）。 結(jié)論： 1.聚集素基因C/C基因型、等位基因C與阿爾茨海默病相關(guān)聯(lián)，且可能是AD的一種危險因素。 2.AD患者血漿聚集素含量較正常對照組明顯升高，該結(jié)論與國外的研究報道一致。聚集素可能在AD的發(fā)生、發(fā)展中起著重要作用。
[Abstract]:Objective: to investigate the content and significance of agglutinin in Alzheimer's disease (AD) and its association with polymorphism of agglutinin gene in Chinese Han nationality. Methods: 1. The cases of AD group and control group were collected. 2. The peripheral venous blood of 3ml was taken from both groups. DNA.4 was extracted from leukocyte genome by hypotonic hemolysis and routine phenol-chloroform method. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to observe the polymorphism of aggregin gene. The levels of plasma aggregates in AD patients (AD group) and the healthy elderly (control group) were detected by Elisa. Results: 1. The C / C genotype of the agglutinin gene in AD group was determined by enzyme linked immunosorbent assay (Elisa). The distribution of allele C was significantly higher than that of the control group (蠂 26.673, P 0.05) (蠂 2 = 15.060, P 0.05), and the genotype C / C and C / C were significantly positively correlated with AD. Conclusion: 1.855-7.320cl 1.855-7.320Cl1.75095Cl1.260-6.0020.2.The level of plasma aggregation in AD group was significantly higher than that in control group (P 0.01). C / C genotype and allele C are associated with Alzheimer's disease and may be a risk factor for AD. 2. Plasma concentration of aggregin in AD patients is significantly higher than that in normal controls. Agglutinin may play an important role in the occurrence and development of AD.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R749.16

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 辛殿祺,朱緒輝,艾軍魁,那彥群,郭應(yīng)祿;Clusterin在前列腺癌LNCaP細(xì)胞中的抗凋亡作用[J];北京大學(xué)學(xué)報(醫(yī)學(xué)版);2004年02期

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