本文選題：血管性癡呆 + 杏花雨��； 參考：《河北大學(xué)》2012年碩士論文

【摘要】：目的：本研究通過(guò)觀察高純度銀杏黃酮注射制劑杏花雨對(duì)缺血再灌注造成的血管性癡呆(VD)大鼠行為及其海馬Bcl-2，Bax蛋白表達(dá)的影響，探討杏花雨對(duì)VD大鼠學(xué)習(xí)記憶能力的影響及可能的作用機(jī)制，旨在為該藥防治VD提供實(shí)驗(yàn)依據(jù)。 方法：采用雙側(cè)頸總動(dòng)脈缺血再灌注+硝普鈉降壓法制作VD模型，模型復(fù)制成功的VD大鼠隨機(jī)分為模型組、杏花雨A組、杏花雨B組、金納多組，另設(shè)假手術(shù)組。術(shù)后第2天開(kāi)始腹腔注射給藥，劑量均為5mL/kg，給藥時(shí)間共計(jì)21天，從給藥第3周開(kāi)始采用Morris水迷宮實(shí)驗(yàn)裝置及動(dòng)態(tài)圖像采集分析系統(tǒng)，由計(jì)算機(jī)記錄各種參數(shù)記錄。水迷宮實(shí)驗(yàn)完成后第2天，各組分別迅速斷頭取腦組織做HE染色，觀察大鼠大腦海馬區(qū)形態(tài)學(xué)改變并做免疫組化檢測(cè)大鼠海馬CA1區(qū)Bcl-2、Bax蛋白表達(dá)。 結(jié)果：1.水迷宮實(shí)驗(yàn)結(jié)果：從水迷宮訓(xùn)練第1天起，各組大鼠逃避潛伏期與假手術(shù)組比較沒(méi)有差別（P0.05）；而第2天各組逃避潛伏期均明顯縮短，模型組與各治療組之間有明顯差別（P0.05）；第3天開(kāi)始成績(jī)比較穩(wěn)定，與模型組比較，杏花雨B與假手術(shù)組逃避潛伏期顯著縮短（P0.01），，杏花雨A組與金納多組逃避潛伏期明顯縮短（P0.05）；第4至6天各組與模型組比較均有極顯著差異（P0.01）；另外，與金納多組相比，杏花雨B組逃避潛伏期短，差異有統(tǒng)計(jì)學(xué)意義（P0.05），杏花雨A組逃避潛伏期較短，但差異無(wú)統(tǒng)計(jì)學(xué)意義（P0.05）；2.病理學(xué)改變：假手術(shù)組大鼠海馬CA1區(qū)未見(jiàn)明顯病理變化。模型組大鼠海馬CA1區(qū)神經(jīng)元大量變性、死亡，呈現(xiàn)深紅色染色，核固縮、深染。杏花雨A、B組、金納多組與模型組相比，海馬CA1區(qū)神經(jīng)元胞體縮小，但形狀與正常接近，變性壞死明顯較輕。而3組之間以杏花雨B組大鼠海馬CA1區(qū)形態(tài)與假手術(shù)組更接近。3.Bcl-2、Bax蛋白表達(dá)：假手術(shù)組大鼠海馬CA1區(qū)可見(jiàn)少量Bcl-2，Bax陽(yáng)性細(xì)胞表達(dá)，模型組Bcl-2，Bax陽(yáng)性細(xì)胞數(shù)量明顯增加（P0.01），其中Bax變化更為顯著，Bcl-2/Bax比值較假手術(shù)組顯著降低（P0.05）；經(jīng)杏花雨A、B及金納多治療后，各治療組大鼠海馬CA1區(qū)Bcl-2陽(yáng)性細(xì)胞數(shù)均有提高（P0.05），杏花雨B組有明顯提高（P0.01）；大鼠海馬CA1區(qū)Bax陽(yáng)性細(xì)胞數(shù)降低，尤其以杏花雨B組降低明顯（P0.01），Bax與Bcl-2的比值也較模型組明顯提高（P0.05）。 結(jié)論： 1.銀杏黃酮可顯著改善VD大鼠認(rèn)知水平，提高學(xué)習(xí)記憶功能能力，同等劑量下，以杏花雨B效果最佳，杏花雨A與金納多效果相當(dāng)。 2.通過(guò)大鼠大腦組織海馬CA1區(qū)病理形態(tài)學(xué)觀察，證實(shí)銀杏黃酮能保護(hù)VD大鼠海馬區(qū)的CA1區(qū)神經(jīng)細(xì)胞，以杏花雨B組效果最佳。 3.免疫組化結(jié)果證實(shí)銀杏黃酮通過(guò)調(diào)節(jié)VD大鼠海馬組織Bcl-2與Bax的蛋白表達(dá)，保護(hù)海馬組織神經(jīng)元，從而減輕腦缺血再灌注對(duì)海馬神經(jīng)元的損傷。其中杏花雨B的這種保護(hù)作用最強(qiáng)，杏花雨A與金納多具有等效性。
[Abstract]:Objective: to observe the effect of high purity ginkgo flavone injection preparation apricot rain on the behavior of vascular dementia rats induced by ischemia and reperfusion and the expression of Bcl-2 Bax protein in hippocampus. To explore the effect of apricot rain on learning and memory ability of VD rats and its possible mechanism in order to provide experimental evidence for the prevention and treatment of VD. Methods: VD model was made by bilateral common carotid artery ischemia reperfusion with sodium nitroprusside. VD rats were randomly divided into three groups: model group, apricot flower rain group A, apricot flower rain group B, Jinnardo group and sham operation group. On the second day after operation, the dosage was 5 mL / kg, and the administration time was 21 days. From the third week of administration, the Morris water maze experimental device and the dynamic image collection and analysis system were used to record the parameters by computer. On the second day after the completion of the water maze experiment, the brain tissues of each group were quickly severed for HE staining. Morphological changes in the hippocampus of the rats were observed and the expression of Bcl-2P Bax protein in the CA1 area of the rat hippocampus was detected by immunohistochemistry. The result is 1: 1. The results of water maze experiment showed that from the first day of water maze training, the escape latency of rats in each group was not different from that of sham operation group (P 0.05), but on the second day, the escape latency of each group was significantly shorter than that of sham operation group. There were significant differences between the model group and the treatment group (P 0.05), and the results of the model group were stable at the beginning of the third day, compared with the model group. The escape latency of apricot flower rain B and sham operation group was significantly shortened (P 0.01), and the escape latency of apricot flower rain group A and Jinnadu group was significantly shortened (P 0.05); on the 4th to 6th day, there was a significant difference between each group and model group (P 0.01); in addition, compared with Jinnaduo group, the escape latency of apricot flower rain group A and Jinnaduo group was significantly shorter than that of Jinnaduo group. The escape latency of group B was shorter than that of group A, but there was no significant difference between group A and group A (P 0.05). Pathological changes: there were no obvious pathological changes in CA1 area of hippocampus in sham operation group. In the model group, the neurons in the hippocampal CA1 area were denatured and died, showing dark red staining, nuclear pyknosis and deep staining. Compared with the model group, the neuronal bodies in the CA1 area of the apricot rain group and Jinnaduo group were smaller, but the shape was close to the normal, and the degeneration and necrosis of the neurons were lighter than that of the model group. The morphology of CA1 in hippocampus of group B was closer to that of sham-operated group. 3. The expression of Bcl-2nBax protein was observed in the CA1 area of hippocampus of rats in sham-operation group, and a small number of Bcl-2mBax positive cells were found in the CA1 area of rats in sham-operation group. In the model group, the number of Bcl-2nBax positive cells increased significantly, and the ratio of Bcl-2 / Bax to Bcl-2 / Bax in the model group was significantly lower than that in the sham-operated group. In each treatment group, the number of Bcl-2 positive cells in the CA1 region of hippocampus was increased, and the number of Bax positive cells in the CA1 region of the hippocampus was decreased in group B, especially in the group B, the ratio of P0.01-Bax to Bcl-2 in group B was also significantly higher than that in the model group. Conclusion: 1. Ginkgo flavonoids could significantly improve the cognitive level and the learning and memory ability of VD rats. At the same dosage, the effect of apricot flower rain B was the best, and the effect of apricot flower rain A was the same as that of Jinnadio. 2. The pathomorphological observation of hippocampal CA1 area in rat brain tissue showed that ginkgo flavonoids could protect CA1 neurons in hippocampus of VD rats. The best effect was in group B of apricot rain. 3. The results of immunohistochemistry showed that ginkgo flavone could protect hippocampal neurons by regulating the expression of Bcl-2 and Bax in hippocampus of VD rats, thereby reducing the injury of hippocampal neurons induced by cerebral ischemia reperfusion. The protective effect of apricot flower rain B is the strongest, and apricot flower rain A is equivalent to Ginado.
【學(xué)位授予單位】：河北大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R285.5;R749.1

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