本文選題：neuroplastin + neuroplastin��； 參考：《神經(jīng)解剖學(xué)雜志》2017年06期

【摘要】：目的:研究neuroplastin65(NP65)敲除對小鼠焦慮樣行為的影響并初步探討其機(jī)制。方法:成年NP65敲除(NP65~(-/-))小鼠和同基因型背景的野生型(wild type,WT)小鼠,通過Western Blot鑒定NP65~(-/-)小鼠;用曠場實驗、探洞實驗和埋珠實驗檢測小鼠的焦慮水平;ELISA檢測小鼠海馬、前額葉皮質(zhì)和紋狀體谷氨酸和5-羥色胺(5-hydroxytryptamine,5-HT)的含量;5-HT合成酶色氨酸羥化酶2(tryptophan hydroxylase 2,TPH2)免疫熒光染色觀察小鼠腦干中縫核5-HT能神經(jīng)元;Western Blot檢測小鼠腦干TPH2的蛋白表達(dá)。結(jié)果:與WT小鼠相比,在曠場實驗中,NP65~(-/-)小鼠進(jìn)入中心區(qū)的次數(shù)顯著減少(P0.001),中心區(qū)停留時間顯著降低(P0.001),運(yùn)動總距離和平均運(yùn)動速度無顯著變化。探洞實驗中,NP65~(-/-)小鼠探洞次數(shù)顯著增加(P0.001)、探洞時間增多(P0.05)、后肢豎立次數(shù)減少(P0.01)和排便次數(shù)增多(P0.001)。埋珠實驗中,NP65~(-/-)小鼠掩埋潛伏期延長(P0.05),埋珠個數(shù)顯著減少(P0.05)。神經(jīng)遞質(zhì)檢測結(jié)果顯示:NP65~(-/-)小鼠海馬、前額葉皮質(zhì)和紋狀體谷氨酸的含量不變,海馬5-HT的含量顯著減少(P0.05),但前額葉皮質(zhì)和紋狀體5-HT的含量沒有顯著性差異。免疫熒光染色觀察到NP65~(-/-)小鼠腦干中縫核TPH2陽性細(xì)胞數(shù)目顯著減少(P0.05)。Western Blot分析顯示:NP65~(-/-)小鼠腦干TPH2的蛋白表達(dá)量顯著降低(P0.05)。結(jié)論:NP65敲除導(dǎo)致小鼠表現(xiàn)出顯著的焦慮樣行為,可能與腦干中縫核TPH2表達(dá)減少而導(dǎo)致海馬5-HT含量降低有關(guān)。
[Abstract]:Aim: to study the effect of neuroplastin 65 (NP65) knockout on anxiety like behavior in mice and its mechanism. Methods: adult NP65 knockout (NP65) and wild type (WTW) mice were identified by Western Blot, and the anxiety level of the mice was detected by open field test, burying test and burying test. The level of anxiety was detected by Elisa. The content of glutamate and 5-hydroxytryptamine (5-HT) in prefrontal cortex and striatum; Immunofluorescence staining of 5-HT synthetase tryptophan hydroxylase 2(tryptophan hydroxylase _ 2 (TPH _ 2) was used to detect the protein expression of TPH2 in mouse brain stem by Western Blot. Results: compared with WT mice, in the open field experiment, the times of entering the central area were significantly decreased, the residence time of the central area was significantly decreased, and the total distance and the average exercise speed were not significantly changed. In the hole exploration experiment, the number of holes in NP65 / -) mice increased significantly (P 0.001), the time of hole probing increased (P 0.05), the number of erections of hind limbs decreased (P 0.01) and the times of defecation increased (P 0.001). In the bead burying experiment, the burying latency of NP65 / r -) mice was prolonged (P0.05), and the number of buried bead decreased significantly (P0.05). The results of neurotransmitters test showed that the content of glutamate in prefrontal cortex and striatum was unchanged in the hippocampus, and the content of 5-HT in hippocampus decreased significantly (P 0.05), but there was no significant difference in 5-HT content between prefrontal cortex and striatum. Immunofluorescence staining showed that the number of TPH2 positive cells in the nucleus raphe nucleus of the NP65 mice was significantly decreased. Western Blot analysis showed that the protein expression of TPH2 in brain stem of the mice was significantly decreased. Conclusion knockout of brain stem NP65 induces significant anxiety like behavior in mice, which may be related to the decrease of TPH2 expression in the raphe nucleus of brain stem and the decrease of 5-HT content in hippocampus.
【作者單位】： 同濟(jì)大學(xué)醫(yī)學(xué)院解剖組胚與神經(jīng)生物學(xué)系;
【基金】：國家自然科學(xué)基金(81371213)
【分類號】：R749.72
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本文編號：1837822